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yzdjyj铁杆木虫 (正式写手)
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[交流]
求助翻译一段农业方面的中译英，悬赏200金币！关于棉花转基因方面的！
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如题所示，关于棉花转基因方面的！只要意思大概对即可！ 由图4可知，经过卡那霉素筛选后的T0代棉花植株进行两代自交筛选后得到5个纤维长度明显变短的纯系，其中3个为E6启动子，2个为35S启动子。五个纯系纤维长度均短于对照，其中E6AS-2、35AS-1显著变短。Southern检测结果表明这5个纯系为1-4个拷贝不等，其中E6AS-1、E6AS-2为单拷贝，其余为多拷贝。定量PCR分析结果表明，反义转基因纯系中GhSUSA1基因在开花后25d被抑制最为明显，开花后10d表达量也均被显著抑制，其余时期转基因株系表达量没有对照高。其中35AS-1株系在整个时期表达量都很低。 对纤维长度、强度和酶活进行了统计分析，由表2可知这5个纯系纤维长度都不同程度的变短了，尤其是E6AS-2和35AS-1，这两个纯系在0DPA表达量就很低，在10DPA表达量也低于其他株系，因为10DPA是纤维伸长最为活跃的时期，推测纤维长度变化与10DPA表达量被显著抑制有关；同时转基因株系在10DPA的酶活也低于对照，酶活测定结果与表达量相一致，与纤维长度呈显著正相关，相关系数为r=0.853。反义转基因株系纤维强度也受到影响，5个株系强度均有所下降，尤其35AS-1下降最为明显，而35AS-1在次生壁初始形成的23DPA表达量最低。将纤维强度和23d相对表达量比较发现，二者呈较高正相关（r=0.847）。这一结果表明蔗糖合酶的抑制不仅能影响纤维伸长而且使纤维强度降低了。 35AS-1除了上述纤维品质方面的变化，成熟棉铃还较对照显著变小了。对35AS-1和35AS-2测定了铃重和百粒重，结果显示35AS-1棉铃重量显著减小，百粒重35AS-1和35AS-2两个纯系均显著减小。说明在35S启动子下，GhSUSA1基因的抑制能够影响种子质量。 正义转基因载体由于是GUS融合载体，转基因后代经GUS染色后筛选获得阳性单株。目前转基因纯系虽然没有完全获得，但就T0代纤维表型来看至少出现了3个纤维显著变长的单株，且纤维长度都在30mm以上。 |
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szb4379
木虫 (著名写手)
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2楼2010-03-03 16:33:20
yzdjyj(金币+2):这位朋友，你翻译实在不怎么样，但还是送你两个金币，以资鼓励！ 2010-03-04 12:03
| We can see from Figure 4, after kanamycin selection after the T0 generation of cotton plants were obtained after two generations of self-selection 5 fiber length significantly shortened pure, of which three of E6 promoter, two for the 35S promoter . Five pure fiber length are shorter than in control, which E6AS-2, 35AS-1 significantly shorter. Southern analysis revealed that these five pure line for 1-4 copies, and include wheelchair E6AS-1, E6AS-2 as a single copy, the remainder being multiple copies. Quantitative PCR analysis showed that the antisense transgenic lines GhSUSA1 pure gene in 25d after flowering was inhibited most obvious expression in volume 10d after flowering were significantly inhibited and the remaining period of the expression of transgenic lines did not control the high. One 35AS-1 strains in the expression levels were low throughout the period. |
3楼2010-03-04 11:06:42
yzdjyj(金币+2):不怎么行，鼓励一下 2010-03-04 12:04
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The fiber length, strength and activity of a statistical analysis, from Table 2 we can see these five pure fiber length is shorter in varying degrees, and in particular the E6AS-2 and 35AS-1, these two pure expression of the 0DPA volume very low, in the 10DPA expression is also lower than the other strains, because 10DPA is the most active period of fiber elongation, suggesting fiber length change and 10DPA expression was significantly inhibited-related; the same time, genetically modified strains of the activity is also in the 10DPA lower than the control, enzyme activity and the expression of determination results are consistent with the fiber length was significantly positively correlated with a correlation coefficient r = 0.853. Antisense transgenic lines also affected fiber strength, 5 lines have decreased strength, in particular, 35AS-1 decreased the most obvious, and 35AS-1 in the secondary wall formation 23DPA the initial expression of the minimum. The fiber strength and 23d relative expression levels of comparison, both showed a high positive correlation (r = 0.847). The results showed that the inhibition of sucrose synthase can not only affect the fiber elongation but also reduces the fiber strength. 35AS-1 In addition to these changes in fiber quality, mature cotton bolls compared with the control was also significantly smaller. Right 35AS-1, and 35AS-2 was determined by boll weight and kernel weight showed 35AS-1 significantly reduced boll weight, kernel weight 35AS-1, and 35AS-2 two pure line were significantly reduced. Indicate under the 35S promoter, GhSUSA1 gene suppression can affect seed quality. Justice transgenic GUS fusion vector because it is vector, transgenic offspring were screened by GUS staining was positive plant. Currently transgenic pure, although not completely available, but on the T0 generation of fiber phenotype of view there were at least three fibers of the plant significantly longer, and the fiber length are more than 30mm. |
4楼2010-03-04 11:08:06
★ ★
yzdjyj(金币+1):鼓励一下！ 2010-03-04 12:04
phyweiw(金币-2):机器翻的，不说明。 2010-03-04 14:14
yzdjyj(金币+1):鼓励一下！ 2010-03-04 12:04
phyweiw(金币-2):机器翻的，不说明。 2010-03-04 14:14
|
We can see from Figure 4, after kanamycin selection after the T0 generation of cotton plants were obtained after two generations of self-selection 5 fiber length significantly shortened pure, of which three of E6 promoter, two for the 35S promoter . Five pure fiber length are shorter than in control, which E6AS-2, 35AS-1 significantly shorter. Southern analysis revealed that these five pure line for 1-4 copies, and include wheelchair E6AS-1, E6AS-2 as a single copy, the remainder being multiple copies. Quantitative PCR analysis showed that the antisense transgenic lines GhSUSA1 pure gene in 25d after flowering was inhibited most obvious expression in volume 10d after flowering were significantly inhibited and the remaining period of the expression of transgenic lines did not control the high. One 35AS-1 strains in the expression levels were low throughout the period. The fiber length, strength and activity of a statistical analysis, from Table 2 we can see these five pure fiber length is shorter in varying degrees, and in particular the E6AS-2 and 35AS-1, these two pure expression of the 0DPA volume very low, in the 10DPA expression is also lower than the other strains, because 10DPA is the most active period of fiber elongation, suggesting fiber length change and 10DPA expression was significantly inhibited-related; the same time, genetically modified strains of the activity is also in the 10DPA lower than the control, enzyme activity and the expression of determination results are consistent with the fiber length was significantly positively correlated with a correlation coefficient r = 0.853. Antisense transgenic lines also affected fiber strength, 5 lines have decreased strength, in particular, 35AS-1 decreased the most obvious, and 35AS-1 in the secondary wall formation 23DPA the initial expression of the minimum. The fiber strength and 23d relative expression levels of comparison, both showed a high positive correlation (r = 0.847). The results showed that the inhibition of sucrose synthase can not only affect the fiber elongation but also reduces the fiber strength. 35AS-1 In addition to these changes in fiber quality, mature cotton bolls compared with the control was also significantly smaller. Right 35AS-1, and 35AS-2 was determined by boll weight and kernel weight showed 35AS-1 significantly reduced boll weight, kernel weight 35AS-1, and 35AS-2 two pure line were significantly reduced. Indicate under the 35S promoter, GhSUSA1 gene suppression can affect seed quality. Justice transgenic GUS fusion vector because it is vector, transgenic offspring were screened by GUS staining was positive plant. Currently transgenic pure, although not completely available, but on the T0 generation of fiber phenotype of view there were at least three fibers of the plant significantly longer, and the fiber length are more than 30mm. |
5楼2010-03-04 11:08:43
