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hnndpt

铁虫 (正式写手)

[交流] 【求助/交流】双酶切buffer问题请教!

MSAP中HpaII和MspI双酶切用什么buffer?
Buffer 10X Tango™能自己稀释吗?
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三磷酸腺苷

铁杆木虫 (职业作家)

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amisking(金币+1): 1-26 21:00
Fermentas的快速内切酶通用一种buffer
2楼2010-01-26 18:28:36
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j2

木虫 (正式写手)

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小木虫(金币+0.5):给个红包,谢谢回帖交流
稀释?是你做多大体系加相应量的buffer吧
修炼ing,当虫仙……
3楼2010-01-26 19:37:54
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天使托

专家顾问 (职业作家)

T.Shen

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小木虫(金币+0.5):给个红包,谢谢回帖交流
amisking(金币+1): 1-26 21:00
应该在买酶的时候会带着一张可以查的图的~
要不上Fermentas网站上查查~
Whenever,Wherever,Anyway!MustRemember:Fight!KeepFighting!NeverGiveUp!
4楼2010-01-26 20:09:48
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hnndpt

铁虫 (正式写手)

感谢各位高手帮忙,晚上已经做上了,明天看结果怎样
5楼2010-01-26 22:28:46
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susizheng

木虫 (著名写手)

我們是糖 甜到憂傷

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小木虫(金币+0.5):给个红包,谢谢回帖交流
http://www.fermentas.com/cn/tools/doubledigest
DoubleDigest™  - to print
Double Digestion with HpaII, MspI
We recommend:
Buffer 1X Tango™
HpaII
MspI
Incubate at 37°C


Other options (presented in the order of decreasing restriction enzyme activity):

1.Buffer G
2-fold excess of HpaII
2-fold excess of MspI
Incubate at 37°C


2.Buffer B
2-fold excess of HpaII
2-fold excess of MspI
Incubate at 37°C
Thank-you,so-blue.
6楼2010-01-26 23:23:56
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qqsvery

木虫 (著名写手)

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引用回帖:
Originally posted by susizheng at 2010-01-26 23:23:56:
http://www.fermentas.com/cn/tools/doubledigest
DoubleDigest™  - to print
Double Digestion with HpaII, MspI
We recommend:
Buffer 1X Tango™
HpaII
MspI
Incubate at 37°C ...

感谢提供网站,信息不少啊!
谎言和真实在河边洗澡,谎言先洗好,穿走了真实的衣服,真实却不肯穿谎言的衣服。后来人们眼里只有穿着真实衣服的谎言,却很难接受赤裸裸的真实。
7楼2010-01-27 17:38:25
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