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Isolation and Determination of Sterols in Rapeseed Oil by HPLC-MS
·¢±íÔÚ£ºAnalytical Letters,42:11,1650 ¡ª 1661

Analytical Letters, 42: 1650¨C1661, 2009
Copyright # Taylor & Francis Group, LLC
ISSN: 0003-2719 print=1532-236X online
DOI: 10.1080/00032710902993811

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Title: Isolation and Determination of Sterols in Rapeseed Oil by HPLC-MS
Author(s): Zhang LF, Liu CF, Meng YB
Source: ANALYTICAL LETTERS   Volume: 42   Issue: 11   Pages: 1650-1661   Published: 2009

Scopus²éÔĽá¹û:

Analytical Letters
Volume 42, Issue 11, 2009, Pages 1650-1661   

Isolation and determination of sterols in rapeseed oil by HPLC-MS

Zhang, L.-F.Show author details, Liu, C.-F.Show author detailsEmail this author, Meng, Y.-B.Show author details Correspondence address
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FN ISI Export Format
VR 1.0
PT J
AU Zhang, LF
   Liu, CF
   Meng, YB
AF Zhang, Li-Feng
   Liu, Can-Fang
   Meng, Yan-Bo
TI Isolation and Determination of Sterols in Rapeseed Oil by HPLC-MS
SO ANALYTICAL LETTERS
LA English
DT Article
DE High-performance liquid chromatography; mass spectrometry; rapeseed
   oil; sterols
ID SOLID-PHASE EXTRACTION; VEGETABLE-OILS; LIQUID-CHROMATOGRAPHY;
   GAS-CHROMATOGRAPHY; FATTY-ACIDS; MILK-FAT; PHYTOSTEROLS; TOCOPHEROLS;
   CHOLESTEROL; SITOSTEROL
AB This article presents the first liquid chromatographic method for the
   identification and quantification of seven phytosterols in rapeseed
   oil. Sterols were identified and quantified by liquid chromatography
   with mass spectrometry (LC-MS) detection in positive atmospheric
   pressure chemical ionization mode. The samples were saponified by
   refluxing with 2N ethanolic potassium hydroxide, and the
   nonsaponificable fraction was extracted with diethyl ether. This
   fraction was subjected to thin-layer chromatography on silica-gel
   plates, and then the band of sterols was isolated and extracted with
   methanol. Sterols were quantified by LC-MS on a Waters Atlantis 5-mu
   mdC18 2.1-x150-mm column with a gradient of acetonitrile/water (0.01%
   acetic acid) at a flow rate of 0.5mL min-1 and column temperature of 30
   degrees C. The method presents values between 123 and 677ng mL-1 for
   detection limits, with relative standard deviations between 4.0% and
   5.4% at a concentration of 5mg L-1 for each sterol. Sterol contents
   were determined in a virgin rapeseed oil, a refined rapeseed oil, a
   rapeseed pomace oil, and a crude rapeseed pomace oil.
C1 [Zhang, Li-Feng; Liu, Can-Fang; Meng, Yan-Bo] Coll Med Xingtai, Dept Pharm, Hebei, Peoples R China.
RP Liu, CF, Coll Med Xingtai, Dept Pharm, Hebei, Peoples R China.
EM liucanfang@163.com
NR 24
TC 0
PU TAYLOR & FRANCIS INC
PI PHILADELPHIA
PA 325 CHESTNUT ST, SUITE 800, PHILADELPHIA, PA 19106 USA
SN 0003-2719
J9 ANAL LETT
JI Anal. Lett.
PY 2009
VL 42
IS 11
BP 1650
EP 1661
DI 10.1080/00032710902993811
PG 12
SC Chemistry, Analytical
GA 463WB
UT ISI:000267462700008
ER

EF

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