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ÄÇλͬÈʰïÎÒ²éÒ»ÏÂÊÇ·ñ±»SCIÊÕ¼£¿ÎÄÕÂÊÇ Isolation and Determination of Sterols in Rapeseed Oil by HPLC-MS ·¢±íÔÚ£ºAnalytical Letters,42:11,1650 ¡ª 1661 Analytical Letters, 42: 1650¨C1661, 2009 Copyright # Taylor & Francis Group, LLC ISSN: 0003-2719 print=1532-236X online DOI: 10.1080/00032710902993811 20½ð±Ò×÷Ϊ¸Ðл£¡ |

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ÊÇµÄ Web of Sci ²éÔĽá¹û: Title: Isolation and Determination of Sterols in Rapeseed Oil by HPLC-MS Author(s): Zhang LF, Liu CF, Meng YB Source: ANALYTICAL LETTERS Volume: 42 Issue: 11 Pages: 1650-1661 Published: 2009 Scopus²éÔĽá¹û: Analytical Letters Volume 42, Issue 11, 2009, Pages 1650-1661 Isolation and determination of sterols in rapeseed oil by HPLC-MS Zhang, L.-F.Show author details, Liu, C.-F.Show author detailsEmail this author, Meng, Y.-B.Show author details Correspondence address |
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Õâ²ÅÊÇ×î׼ȷ¡¢ÍêÕûµÄ½á¹û£º FN ISI Export Format VR 1.0 PT J AU Zhang, LF Liu, CF Meng, YB AF Zhang, Li-Feng Liu, Can-Fang Meng, Yan-Bo TI Isolation and Determination of Sterols in Rapeseed Oil by HPLC-MS SO ANALYTICAL LETTERS LA English DT Article DE High-performance liquid chromatography; mass spectrometry; rapeseed oil; sterols ID SOLID-PHASE EXTRACTION; VEGETABLE-OILS; LIQUID-CHROMATOGRAPHY; GAS-CHROMATOGRAPHY; FATTY-ACIDS; MILK-FAT; PHYTOSTEROLS; TOCOPHEROLS; CHOLESTEROL; SITOSTEROL AB This article presents the first liquid chromatographic method for the identification and quantification of seven phytosterols in rapeseed oil. Sterols were identified and quantified by liquid chromatography with mass spectrometry (LC-MS) detection in positive atmospheric pressure chemical ionization mode. The samples were saponified by refluxing with 2N ethanolic potassium hydroxide, and the nonsaponificable fraction was extracted with diethyl ether. This fraction was subjected to thin-layer chromatography on silica-gel plates, and then the band of sterols was isolated and extracted with methanol. Sterols were quantified by LC-MS on a Waters Atlantis 5-mu mdC18 2.1-x150-mm column with a gradient of acetonitrile/water (0.01% acetic acid) at a flow rate of 0.5mL min-1 and column temperature of 30 degrees C. The method presents values between 123 and 677ng mL-1 for detection limits, with relative standard deviations between 4.0% and 5.4% at a concentration of 5mg L-1 for each sterol. Sterol contents were determined in a virgin rapeseed oil, a refined rapeseed oil, a rapeseed pomace oil, and a crude rapeseed pomace oil. C1 [Zhang, Li-Feng; Liu, Can-Fang; Meng, Yan-Bo] Coll Med Xingtai, Dept Pharm, Hebei, Peoples R China. RP Liu, CF, Coll Med Xingtai, Dept Pharm, Hebei, Peoples R China. EM liucanfang@163.com NR 24 TC 0 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 325 CHESTNUT ST, SUITE 800, PHILADELPHIA, PA 19106 USA SN 0003-2719 J9 ANAL LETT JI Anal. Lett. PY 2009 VL 42 IS 11 BP 1650 EP 1661 DI 10.1080/00032710902993811 PG 12 SC Chemistry, Analytical GA 463WB UT ISI:000267462700008 ER EF ÆäÖÐUT ISI:000267462700008¼´ÊÇÎÄÕµÄΨһÊÕ¼ºÅ£¡Ò²ÊÇÎÄÕ±»SCI¼ìË÷µÄ×îÓÐЧƾ֤¡£ [ Last edited by yicaoting on 2010-1-19 at 18:13 ] |
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