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Several reasons could explain these discrepancies:for instance,it is possible that the overexpression strategies used by ref.5 could have distinct consequences compared with those that are extrapolated from our deletion studies.Additionally,in our work,miR-223 is absent in the entire granulocytic lineage in a mouse,whereas ref.5 manipulated expression of miR-223 in a leukaemic cell line resembling an early granulocyte progenitor.This issue is more relevant given that our data provide evidence for distinct functions of miR-223 during different stages of myeloid cell development,during which microRNA concen- trations change dynamically.Future experiments aimed at manipulat- ing miR-223 expression at defined granulocyte differentiation stages will be useful in determining the multiple roles of this microRNA. [ Last edited by luizing on 2010-1-10 at 21:03 ] |
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luizing(½ð±Ò+10,VIP+0): 1-11 20:44
luizing(½ð±Ò+10,VIP+0): 1-11 20:44
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