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Phytochemical Analysis
Volume 20 Issue 4, Pages 338 - 343
Published Online: 27 May 2009

Copyright 2010 John Wiley & Sons, Ltd.

Qualitative and quantitative determination of seven triterpene acids in Eriobotrya japonica Lindl. by high-performance liquid chromatography with photodiode array detection and mass spectrometry (p 338-343)
Er-Na Li, Jian-Guang Luo, Ling-Yi Kong
Published Online: May 27 2009 12:05AM
DOI: 10.1002/pca.1134



A simple and accurate HPLC-PAD method has been established for the simultaneous quantification of seven bioactive triterpene acids in leaves of Eriobotrya japonica Lindl. The separation was performed on an Ultimate XB-C18 column by gradient elution with aqueous methanol:formic acid. The method was validated for linearity, precision, accuracy, and limits of detection and quantification. The developed method was successfully applied to the quantification of seven constituents in 11 samples of E. japonica collected from different provinces of China.
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wwzhe

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