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1.Domoic acid is produced by diatoms of the genus Pseudo-nitzschia (Bates et al., 1989) during algal blooms.
2.Then domoic acid is ingested by shellfish and accumulates in their edible tissue.
3.However, these methods are often specialized, expensive, cumbersome or time consuming, and not always suited to determination of domoic acid in complex matrices.
4.However, the development of simple and general protocol to immobilize MIP film on QCM crystal is still highly demanded.
5.The FT-IR spectrum of the m-MIP film was shown in supplementary materials Figure S1, which provides more insight into the structure of the m-MIP film.
6.The strong peaks at 1606 cm-1 and 1510 cm-1 are contributed to the C=C skeletal vibrations of the aromatic ring, while the weak peaks at 1284 cm-1 and 1343 cm-1 are contributed to CH2 scissoring vibration.
7.The broad band between about 2900 cm-1 and 3400 cm-1 may be assigned to free -OH (phenolic hydroxyl) or -NH2 stretching vibration.
8.As shows in Figure 5, it is apparent that the adsorption capacities of m-MIP for domoic acid and phthalic acids are different.
9.We propose that the difference of affinity between domoic acid and phthalic acids toward m-MIP should be attributed to the binding sites within m-MIP.
10.We prepared mussel extracts and spiked them with different concentrations of domoic acid.
11.In order to reuse the m-MIP QCM sensor after each determination, a short regeneration step was performed using 10 mL of water containing 5 % acetic acid and 10 mL of deionized water.
12.The results (supplementary materials Figure S3) showed that the m-MIP QCM sensor could be used many times and the initial adsorption capacity was maintained.
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