24小时热门版块排行榜

返回列表

当前主题已经存档。

zhao0919

金虫 (正式写手)

应助: 0 (幼儿园)
金币: 872.6
红花: 5
帖子: 455
在线: 13.7小时
虫号: 757984
注册: 2009-04-26
性别: GG
专业: 放射医学

[交流] 求助一段英文翻译，共四句话，急用！

Two MCF-7 sub-lines MN1 and MDD2 were generously provide by Dr Galmarini (Inserm 453, Laboratoire de Cytologie Analytique, France) and cultured in DMEM
media (Winsent (St Bruno, Qc)) supplemented with 10% SVF. MDD2 line derives from the transfection of MCF-7 cells with a dominant negative mutant of p53 (pCMV-DDp53; mut-p53) that codes for a non-functional p53. MN1 line is the control line transfected with the empty plasmid. Some MN1 cells were transitionally transfected with pCDNA Bcl-2 using the lipofectamin system from Invitrogen (Burlington, Ont.). The human lung carcinoma H1299 line (ATCC (Manassas, VA)) does not express p53 and was cultured in RPMI media (Winsent (St Bruno, Qc))
supplemented with 10% SVF. Human colonic carcinoma cells HCT116 and the p21 null HCT116p21K/K lines were a gift to our laboratory from Dr Vogelstein B. (John
Hopkins University, Baltimore, MD), and were cultured into McCoy media (Wisent (St Bruno, Qc)), supplemented with 10% SVF.

回复此楼