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4. Conclusions The above work provides evidence for the complexation between b-lg and chitosan under low ionic strength conditions. Results indicate that soluble/insoluble complexes were formed via complex coacervation at pH values above the isoelectric point of the b-lg. These complexes were responsible for the enhanced aggregation/denaturation behaviour of b-lg in the presence/absence of 100mM NaCl on heating at pH values in the range 5.5¨C7.0. In contrast, enhanced stability to thermal processes was obtained with heating b-lg in the presence/absence of 100mM NaCl and chitosan in the pH range 4.0¨C5.1. Further studies are required to elucidate the effects of chitosan on the structural conformation of b-lg when subjected to heat, particularly in the pH range below the proteins isoelectric point. |
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