| 查看: 423 | 回复: 4 | |||
| 当前主题已经存档。 | |||
| 当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖 | |||
sunlili1886银虫 (小有名气)
|
[交流]
【求助】麻烦各位
|
||
|
请问各位大侠：谁能帮我找一下这篇文献 Optimization of a quantitative HPLC determination of potentially anticarcinogenic flavonoids in vegetables and fruits. Author: Hertog, M.G.L. : Hollman, P.C.H. : Venema, D.P. Citation: J-agric-food-chem. Washington, D.C. : American Chemical Society. Sept 1992. v. 40 (9) p. 1591-1598. 非常感谢！  |
回复此楼» 猜你喜欢
一志愿河北工业大学0817化工278分求调剂
已经有15人回复
-
085601材料工程找调剂
已经有15人回复
-
材料与化工272求调剂
已经有15人回复
-
085600 286分 材料求调剂
已经有10人回复
-
南京大学化学调剂
已经有14人回复
-
一志愿北京工业大学，324分求调剂
已经有6人回复
-
求调剂
已经有5人回复
-
281求调剂
已经有6人回复
-
26申博自荐
已经有6人回复
-
一志愿哈尔滨工业大学材料与化工方向336分
已经有10人回复
sunlili1886
银虫 (小有名气)
- 应助: 0 (幼儿园)
- 金币: 1452.2
- 散金: 32
- 帖子: 175
- 在线: 248.5小时
- 虫号: 725299
- 注册: 2009-03-18
- 专业: 晶状体与白内障
5楼2009-12-29 12:12:58
xiaofeng3537
金虫 (正式写手)
- 应助: 0 (幼儿园)
- 金币: 659.3
- 红花: 1
- 帖子: 406
- 在线: 59.3小时
- 虫号: 463420
- 注册: 2007-11-20
- 专业: 药物分析
★ ★
小木虫(金币+0.5):给个红包，谢谢回帖交流
472514568(金币+1,VIP+0):谢谢回帖！ 12-25 17:24
小木虫(金币+0.5):给个红包，谢谢回帖交流
472514568(金币+1,VIP+0):谢谢回帖！ 12-25 17:24
|
Optimization of a quantitative HPLC determination of potentially anticarcinogenic flavonoids in vegetables and fruits. Author: Hertog, M.G.L. : Hollman, P.C.H. : Venema, D.P. Citation: J-agric-food-chem. Washington, D.C. : American Chemical Society. Sept 1992. v. 40 (9) p. 1591-1598. Abstract: A rapid method based on RP-HPLC with UV detection is presented for the quantitative determination of five major flavonoid aglycons, viz. quercetin, kaempferol, myricetin, luteolin, and apigenin, in freeze-dried vegetables and fruits, after acid hydrolysis of the parent glycosides. Completeness of hydrolysis and extraction have been optimized by testing systematically different conditions such as acid concentration, reaction period, and methanol concentration in the extraction solution using samples containing various types of flavonoid glycosides. Optimum hydrolysis conditions are presented for flavonol glucuronides, flavonol glucosides, and flavone glycosides. Identity of the flavonoids was confirmed with diode array. Repeatability of the method was good, with coefficients of variation of 2.5-3.1% for quercetin, 4.6-5.6% for kaempferol, 4.6% for myricetin, 3.3% for luteolin, and 2.8% for apigenin. CV of the within-laboratory reproducibility was less than 2 times the CV of repeatability. Recoveries of the flavonols quercetin, kaempferol, and myricetin ranged from 77 to 110%, and recoveries of the flavones apigenin and luteolin ranged from 99 to 106%. The method presented allows a fast, quantitative, and reproducible determination of five flavonoids in freeze-dried foods.SP$ISSN: 0021-8561SP$VOLUME: 40SP$ISSUE: 9SP$PAGE1: 1591 |
3楼2009-12-25 17:01:33
4楼2009-12-25 17:41:38
20