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Trehalose supports the growth of Thermus thermophilus strain HB27, but the absence of obvious genes for the hydrolysis of this disaccharide in the genome led us to search for enzymes for such a purpose. We expressed a putative α-glucosidase gene (TTC0107), characterized the recombinant enzyme, and found that the preferred substrate was α-1,1-trehalose, a new feature among α-glucosidases.Trehalose was not, however, a substrate for the highly homologous α-glucosidase from T. thermophilus strain GK24. |
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