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Primer extension analysis of the qsrP, acfA, and ribB promoters. Lanes G, A, T, and C, sequencing ladder from plasmid DNA; lane 1, RNA from E. coli JM109/pSC300 (luxR)/pSUP102 carrying the promoter of interest with 3-oxo-C6-HSL; lane 2, RNA from E. coli JM109/pSC300 (luxR)/pSUP102 carrying the promoter of interest without 3-oxo-C6-HSL; lane 3, RNA from V. fischeri MJ211 (luxI) with 3-oxo-C6-HSL; lane 4, RNA from V. fischeri MJ211 (luxI) without 3-oxo-C6-HSL; lane 5, no RNA (negative control). The results are representative of the results of assays done in duplicate |
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