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fansanjin

新虫 (初入文坛)

[交流] 油红O染色步骤 已有2人参与

请问有谁在脂肪细胞里面做个油红O染色,急求步骤。

[ Last edited by 1949stone on 2009-11-21 at 21:32 ]
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xiaomi1208

金虫 (正式写手)

引用回帖:
Originally posted by fansanjin at 2009-11-21 21:27:33:
请问有谁在脂肪细胞里面做个油红O染色,急求步骤。

[ Last edited by 1949stone on 2009-11-21 at 21:32 ]

你好  请问油红O是如何配制的  浓度是多少啊  我现在要先用油红O染色  在用苏木素复染  但是都没有查如何到配制  请虫友帮帮忙   谢谢啊
2楼2010-03-24 23:46:21
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fansanjin

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dhd997(金币+1):谢谢交流。 2010-06-17 12:28:17
Preparing Oil Red O Stain
Prepare a stock solution by weighing out 300mg of Oil Red O powder and adding this to 100 mL
of 99% isopropanol. This stock solution is stable one year from the date it was made.
In the fume hood, mix 3 parts of Oil Red O stock solution with 2 parts DI water. Incubate 10
minutes at room temperature. This working solution is only stable for 2 hours.
Place a piece of Whatman filter paper in a funnel above a vessel.
Filter the Oil Red O working solution completely through the filter funnel.
3楼2010-06-17 10:09:56
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xiaojinping

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小木虫: 金币+0.5, 给个红包,谢谢回帖
那请问做的怎么样
4楼2017-10-17 20:09:04
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