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youpractise

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[交流] 完成，谢谢！

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Expression of bgt gene in transgenic birch (Betula platyphylla Suk.)
African Journal of Biotechnology Vol. 8 (15), pp. 3392-3398, 4 August 2009

[ Last edited by youpractise on 2009-11-17 at 15:36 ]
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zqfalcon

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youpractise(金币+5,VIP+0): 11-17 09:04
FN ISI Export Format
VR 1.0
PT J
AU Zeng, FS
   Zhan, YG
   Nan, N
   Xin, Y
   Qi, FH
   Yang, CP
AF Zeng, Fansuo
   Zhan, Yaguang
   Nan, Nan
   Xin, Ying
   Qi, Fenghui
   Yang, Chuanping
TI Expression of bgt gene in transgenic birch (Betula platyphylla Suk.)
SO AFRICAN JOURNAL OF BIOTECHNOLOGY
LA English
DT Article
DE Transgenic birch; transgene; copy number; ELISA; insecticidal activity
ID COPY NUMBER; STABILITY; PLANTS; INTEGRATION; TRANSFORMATION; INSECT;
   GENOME
AB Study on the characteristics of integration and expression is the basis
   of genetic stability of foreign genes in transgenic trees. To obtain
   insight into the relationship of transgene copy number and expression
   level, we screened 22 transgenic birch lines. Southern blot analysis of
   the transgenic birch plants indicated that the copy number of bgt
   varies from one to four, of which 18.2% were single copy. Copy number
   of bgt and gus was different in 68% of transgenic birch plants,
   indicating that rearrangement or partial deletion appeared in the
   process of T-DNA integration. Transcriptional expression of bgt gene in
   transgenic birch plants was analyzed by Northern blot. TGS has been
   found in two transgenic lines with one and four copies of bgt. The
   ELISA result showed that the BGT protein expression level in the
   transgenic birch plants ranged from 0.000 to 0.283% of total soluble
   protein. In contrary to most studies, this research showed no
   significant correlation was found between copy number and expression
   level of bgt gene. Effective resistance of transgenic plants against
   Lymantria dispar was verified in feeding bioassays with the insects.
   Bioassays results were mostly consistent with the expression level of
   BGT insecticidal protein detected by ELISA and Western blot in
   transgenic birch. The paper showed transgenic birch had the high lethal
   effect on gypsy moth larvae ( L. dispar). The practicality of this work
   will benefit not only the birch producers, but also the environment
   worldwide.
C1 [Zeng, Fansuo; Zhan, Yaguang; Yang, Chuanping] Minist Educ, Key Lab Forest Tree Improvement & Biotechnol, Harbin 150040, Peoples R China.
   [Zeng, Fansuo; Zhan, Yaguang; Nan, Nan; Qi, Fenghui] NE Forestry Univ, Coll Life Sci, Harbin 150040, Peoples R China.
   [Xin, Ying] NE Forestry Univ, Coll Forestry, Harbin 150040, Peoples R China.
RP Zhan, YG, Minist Educ, Key Lab Forest Tree Improvement & Biotechnol,
   Harbin 150040, Peoples R China.
EM youpractise@126.com
FU National Nature Science Foundation of China [30471413, 30872045];
   Specialized Research Fund for the Doctoral Program of Higher Education
   [200802251038]
FX This work was supported by the National Nature Science Foundation of
   China(NO: 30471413 and 30872045) and Specialized Research Fund for the
   Doctoral Program of Higher Education (NO: 200802251038).
NR 28
TC 0
PU ACADEMIC JOURNALS
PI VICTORIA ISLAND
PA P O BOX 5170-00200 NAIROBI, VICTORIA ISLAND, LAGOS 73023, NIGERIA
SN 1684-5315
J9 AFR J BIOTECHNOL
JI Afr. J. Biotechnol.
PD AUG 4
PY 2009
VL 8
IS 15
BP 3392
EP 3398
PG 7
SC Biotechnology & Applied Microbiology
GA 500ML
UT ISI:000270306200002
ER

EF
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ljl8209

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.  标题: Expression of bgt gene in transgenic birch (Betula platyphylla Suk.)
作者: Zeng FS, Zhan YG, Nan N, et al.
来源出版物: AFRICAN JOURNAL OF BIOTECHNOLOGY   卷: 8   期: 15   页: 3392-3398   出版年: AUG 4 2009
被引频次: 0  


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youpractise

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这篇的收录号，及现在收录到那期了
谢谢
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3楼2009-11-16 19:12:52
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ljl8209

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出版商: ACADEMIC JOURNALS, P O BOX 5170-00200 NAIROBI, VICTORIA ISLAND, LAGOS 73023, NIGERIA  
学科类别: Biotechnology & Applied Microbiology  
IDS 号: 500ML  
ISSN: 1684-5315
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