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youpractise金虫 (正式写手)
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帮忙查查这篇论文是否已被SCI收录，同时帮看看这个杂志收录到那期了，谢谢！ Expression of bgt gene in transgenic birch (Betula platyphylla Suk.) African Journal of Biotechnology Vol. 8 (15), pp. 3392-3398, 4 August 2009 [ Last edited by youpractise on 2009-11-17 at 15:36 ] |
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FN ISI Export Format VR 1.0 PT J AU Zeng, FS Zhan, YG Nan, N Xin, Y Qi, FH Yang, CP AF Zeng, Fansuo Zhan, Yaguang Nan, Nan Xin, Ying Qi, Fenghui Yang, Chuanping TI Expression of bgt gene in transgenic birch (Betula platyphylla Suk.) SO AFRICAN JOURNAL OF BIOTECHNOLOGY LA English DT Article DE Transgenic birch; transgene; copy number; ELISA; insecticidal activity ID COPY NUMBER; STABILITY; PLANTS; INTEGRATION; TRANSFORMATION; INSECT; GENOME AB Study on the characteristics of integration and expression is the basis of genetic stability of foreign genes in transgenic trees. To obtain insight into the relationship of transgene copy number and expression level, we screened 22 transgenic birch lines. Southern blot analysis of the transgenic birch plants indicated that the copy number of bgt varies from one to four, of which 18.2% were single copy. Copy number of bgt and gus was different in 68% of transgenic birch plants, indicating that rearrangement or partial deletion appeared in the process of T-DNA integration. Transcriptional expression of bgt gene in transgenic birch plants was analyzed by Northern blot. TGS has been found in two transgenic lines with one and four copies of bgt. The ELISA result showed that the BGT protein expression level in the transgenic birch plants ranged from 0.000 to 0.283% of total soluble protein. In contrary to most studies, this research showed no significant correlation was found between copy number and expression level of bgt gene. Effective resistance of transgenic plants against Lymantria dispar was verified in feeding bioassays with the insects. Bioassays results were mostly consistent with the expression level of BGT insecticidal protein detected by ELISA and Western blot in transgenic birch. The paper showed transgenic birch had the high lethal effect on gypsy moth larvae ( L. dispar). The practicality of this work will benefit not only the birch producers, but also the environment worldwide. C1 [Zeng, Fansuo; Zhan, Yaguang; Yang, Chuanping] Minist Educ, Key Lab Forest Tree Improvement & Biotechnol, Harbin 150040, Peoples R China. [Zeng, Fansuo; Zhan, Yaguang; Nan, Nan; Qi, Fenghui] NE Forestry Univ, Coll Life Sci, Harbin 150040, Peoples R China. [Xin, Ying] NE Forestry Univ, Coll Forestry, Harbin 150040, Peoples R China. RP Zhan, YG, Minist Educ, Key Lab Forest Tree Improvement & Biotechnol, Harbin 150040, Peoples R China. EM youpractise@126.com FU National Nature Science Foundation of China [30471413, 30872045]; Specialized Research Fund for the Doctoral Program of Higher Education [200802251038] FX This work was supported by the National Nature Science Foundation of China(NO: 30471413 and 30872045) and Specialized Research Fund for the Doctoral Program of Higher Education (NO: 200802251038). NR 28 TC 0 PU ACADEMIC JOURNALS PI VICTORIA ISLAND PA P O BOX 5170-00200 NAIROBI, VICTORIA ISLAND, LAGOS 73023, NIGERIA SN 1684-5315 J9 AFR J BIOTECHNOL JI Afr. J. Biotechnol. PD AUG 4 PY 2009 VL 8 IS 15 BP 3392 EP 3398 PG 7 SC Biotechnology & Applied Microbiology GA 500ML UT ISI:000270306200002 ER EF |
9楼2009-11-16 20:13:10
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3楼2009-11-16 19:12:52
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4楼2009-11-16 19:36:34
