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canfeng2008

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专业: 蛋白质组学

[交流] 求达人英译汉，谢谢

Prior to statistical analysis the data was normalised to the housekeeping genes glyceraldehyde 3-phosphate dehydrogenase (GAPDH), ribosomal phosphoprotein large P0 subunit (RPLP0) and transferrin receptor (TFRC). The normalisation factorwas calculated as the geometric mean of the three housekeeping genes. In addition a fourth root transformation of the data was conducted in order to reduce heteroscedasticity. In order to test differential expression at the different time points, the linear model; yijk=μ+αi+βj+εijk, was estimated for each gene separately on the normalised and transformed data. The y is the gene expression at time point i, in experiment j and parallel k. μ is the overall mean, αi is the effect of time point i, βj is the effect of experiment j and εijk is the random error between parallels from the same experiment (i=1,2,…, 5, j=1,2,…,4,and k=1,2,3). The experiment term is incorporated in order to correct for potential differences in maturity of isolated stromal–vascular cells.
In the following the estimated time effect is referred to as the estimated expression level.The fold change between two time points was calculated as the log2 of the ratio of the estimated expression levels for the two days, after transforming back to the original scale. For the proliferation phase the fold change was calculated between days 1 and 3, whereas for the differentiation phase fold changes were calculated between days 3 and 10. Genes were reported as significantly differentially expressed at different time points when the population marginal means were significantly different with level 0.05 according to the Tukey–Kramer test for multiple comparisons.

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1楼 2009-11-04 22:38:59

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lixiaod001

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canfeng2008(金币+2,VIP+0):在线翻的吧，很多不对，但还是谢谢 11-8 12:11

【自己整理一下，希望有帮助】
在此之前的统计分析数据正常化的看家基因甘油三磷酸脱氢酶（GAPDH），核糖体磷蛋白大亚基P0（RPLP0）和转铁蛋白受体（TFRC）。在正常化factorwas计算三个看家基因的几何平均数。此外，一项数据的第四根进行改造，以减少异方差。为了测试在不同的时间点差异表达的线性模型; yijk =μ+课表+βj+εijk，是为每一个基因的估计分别就正常化和变换后的数据。 Y染色体的基因表达在时间点，我在J和平行试验，光μ的是整体的意思，课表是时间点，我的影响，βj是实验效果J和εijk是随机误差之间的相似之处，从同样的实验（1 = 1,2，...，5，歼= 1,2 ，...，4，和k = 1,2,3）。这个词纳入为了纠正潜在的差异成熟孤立的基质血管细胞的实验。

在下面的估计时间效应被称为水平下估计表达倍两个时间点之间的变化是由于对两日估计的表达水平计算比率log2改造后回到原来的规模。对于扩散阶段的褶皱变化，计算天之间，而1和3的分化阶段多端的变化，有3至10天计算。据报道，基因差异显着不同时间点是在人口边际的手段与水平显着不同0.05根据图基，多重比较试验克莱默表示。