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canfeng2008银虫 (正式写手)
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Assessment of the metabolic activity of differentiating preadipocytes confirmed our previous gene expression and histocytochemical results (Samulin et al., 2008b), showing that preadipocytes almost exclusively stored FAs in the form of TAG. In fact, only 1.3% of the [14C] 16:0 taken up by the cells was utilised for energy through β- oxidation and the remaining 98.7% was stored in the cells. Due to the low total oxidation in the differentiating preadipocytes, analysis of ASPs by HPLC did not give conclusive results on oxidation end products. Moreover, 82.2% of the stored [14C] 16:0 was recovered in TAG, whereas the remaining 16.5% was recovered in PL. Only small immeasurable amounts of [14C] 16:0 could be found in the remaining lipid classes. In order to investigate the degree of FA modifications present during late adipogenesis the TAG and PL fractions were separated by silver ion TLC. Interestingly, 54% of the radioactive 16:0 had been desaturated and elongated into 18:1n−9 in both TAG and PL fractions . |
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前脂肪细胞分化证实了我们原先的基因表达和组织产物代谢活动的评估显示前脂肪细胞以附属物的形式几乎单独的贮藏脂肪。事实上，1.3%[14C] 16:0通过细胞β氧化消耗能量，剩下的98.7%储存在细胞中。由于在前脂肪细胞分化中低的氧化作用，通过高效液相色谱分析法不能给出最后的氧化产物的结果，而且82.2%[14C] 16:0的储藏被附着物回收，而剩下的16.5%仍然在原处，仅仅少量不可测量的[14C] 16:0仍然在类脂物中被发现，为了研究FA变更的程度，后期脂肪通过阴离子分别生成TAG和PL。有趣的是，54%的放射性16：0已经被稀释和拉长到18:1n−9在TAG和PL中。 因为找不到[14C] 16:0是什么意思，这点就没翻译。你参考下吧 |
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yzhang1986
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yzhang1986
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