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关于审稿人提出DMEM培养液的问题
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The freshly isolated chondrocytes were plated at a density of 2×104 cells/well, which had been immersed previously in plastic dishes filled with culture medium (2000 U/ml in Dulbecco’s Modified Eagle’s Medium, supplemented with 2 mM glutamine, 0.1 mM penicillin, 0.1 mM streptomycin and 10% fetal calf serum). （我原文整个一句话） Briefly, cells were isolated by type I collagenase digestion (2000 U/ml in Dulbecco’s modified Eagle’s medium (DMEM), supplemented with 2 mM glutamine, 0.1 mM penicillin and 0.1 mM streptomycin, for 18 h at 37 ºC) from the metacarphophalangeal joint of 18-36 month-old cattle obtained at abattoir slaughter. （已有的文献） p. 5 last 5 lines - please check this text as the DMEM is not present at 2000U/ml, and concentrations of pen/strep are typically given in Units. （审稿人的意见，我还是不明白审稿人的意见？） |
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