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]求乳杆菌培养基配方？ [ Last edited by amisking on 2009-10-15 at 11:42 ] |
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5楼2009-10-15 13:51:51
dingqian03
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1. Acetobacter Medium (醋酸菌培养基) Glucose (葡萄糖) 100g Yeasst extract (酵母膏) 10g CaCO3 20g Agar (琼脂) 15g Distilled water (蒸馏水) 1000ml Adjust (调) pH to 6.8 适用范围：恶臭醋酸杆菌混浊变种 2. Nutrient Agar (营养肉汁琼脂) Pepton (蛋白胨) 5g Beef extract (牛肉膏) 30g NaCl 5g Agar (琼脂) 15g Distilled water (蒸馏水) Adjust (调) pH to 7.0-7.2 [Note]：When cultivation of Bacillus，5mg of to MnSO4.H2O may be added . It is favorable to promote spore formation . 适用范围：产气气杆菌、粪产碱杆菌、蜡状芽孢杆菌、蜡状芽孢杆菌蕈状变种、地衣形芽孢杆菌、巨大芽孢杆菌、多粘芽孢杆菌、尘埃芽孢杆菌、短小芽孢杆菌、嗜热脂肪芽孢杆菌、枯草芽孢杆菌、枯草芽孢杆菌深黑变种、苏云金芽孢杆菌、苏云金芽孢杆菌蜡螟亚种（青虫菌）、苏云金芽孢杆菌戈尔斯德变种、苏云金芽孢杆菌猝倒亚种、产氨短杆菌、黄色短杆菌、谷氨酸棒状杆菌、北京棒杆菌、大肠埃希氏菌（大肠杆菌）、铜绿假单胞菌（绿脓杆菌）、凸形假单胞杆菌、荧光假单胞菌、弯曲假单胞菌、恶臭假单胞菌、假单胞杆菌、藤黄八叠球菌、亚黄八叠球菌、尿素八叠球菌、金黄色葡萄球菌、运动发酵单孢菌 4. Corn Meal Medium (玉米粉培养基) Maize flour (玉米粉) 5g Peptone (蛋白胨) 0.1g Glucose (葡萄糖) 1g Tap water (自来水) 1000ml [Note]:Boil the mixture in autoclave at 121℃ for 1 hr. distribute the medium into 18ⅹ18 mm tubes , each contains 10 ml of the liquid , then autoclave at 121℃ for 1 hr . again (15磅蒸煮1小时,分装入18ⅹ18毫米试管,每管深度达6厘米。15磅再次灭菌15小时。) 5. Lactic-bacteria Medium I (乳酸菌培养基 I ) Yeast extract (酵母膏) 7.5g Peptone (蛋白胨) 7.5g Glucose (葡萄糖) 10g KH2PO4 2g Tomato juice (西红柿汁) 100ml Tween (吐温) 80 0.5ml Distilled water (蒸馏水) 900ml pH 7.0 适用范围：植物乳杆菌（胚芽乳杆菌）、嗜热乳酸链球菌 6. Lactic-bacteria Midium Ⅱ (乳酸菌培养基 Ⅱ) Lacto-casein peptone (乳酪蛋白胨) 10g Beef extract (蛋白胨) 10g Yeast extract (酵母膏) 5g Glucose (葡萄糖) 5g Tween (吐温) 80 1g K2HPO4 2g Na-acetate (醋酸钠) 5g Diamine citrate (柠檬酸二胺) 2g MgSO4.7H2O 0.2g MnSO4.H2O 0.05g Distilled water (蒸馏水) 1000m pH 6.5-6.8 适用范围：植物乳杆菌（胚芽乳杆菌） 7. Peotone Glucose Yeast extract Medium PGY （蛋白胨、酵母膏、葡萄糖培养基） Peptone（蛋白胨） 10g Yeast extract （酵母膏） 5g Glucose （葡萄糖） 1g Distilled water （蒸馏水） 1L 8. Glycerol Agar （甘油琼脂） Peptone （蛋白胨） 5g Beef extract （酵母膏） 3g Glycerol （甘油） 20g Top water （自来水） 1000ml Agar （琼脂） 15g pH 7.0-7.2 9. Rhizobium medium （根瘤菌培养基）AS 9 Yeast eztract （酵母膏） 1g Soil eztract （土壤浸提液） 200ml Mannitol （甘露醇） 10g Agar （琼脂） 15g Distilled water （蒸馏水） 800ml pH 7.2 [Note]：Soil extract：Suspend 50g finely and dried gardon soil in 200ml of tap water. Autoclave at 121℃ for 1 hr. Decant through cotton-cloth, filler though paper, make up volume to 200ml. Resterilize for 20 minutes at 121℃ , then mixed with othringredients and distributed. (土壤浸提液的制法：取土壤50克，加水200毫升，15磅蒸煮1小时，经滤纸过滤后加水补足到200毫升。) 适用范围：大豆根瘤菌（慢生型）、豇豆慢生根瘤菌、花生根瘤菌、紫云英根瘤菌、 大豆根瘤菌（快生型）、大豆根瘤菌、豌豆根瘤菌、苜蓿根瘤菌、田菁根瘤菌 10. Mannitol Agar (甘露醇琼脂) Yeast extract (酵母膏) 5g Peptone (蛋白胨) 3g Mannitol (甘露醇) 25g Agar (琼脂) 15g Distilled water (蒸馏水) 1000ml 11. Glucose Asparagine （葡萄糖、天门冬素琼脂） Glucose （葡萄糖） 10g Asparagine （天门冬素） 0.5g K2HPO4 0.5g Water （水） 1000ml pH 7.2-7.4 适用范围：刺孢小单孢菌绛红变种、紫色小单孢菌（绛红小单孢菌） 12. Gause′s Synthetic Agar （高氏合成一号琼脂） KNO3 1g Soluble starch（可溶性淀粉） 20g K2HPO4 0.5g MgSO4.7H2O 0.5g NaCl 0.5g FeSO4 0.01g Agar （琼脂） 20g water （水） 1000ml pH 7.2-7.4 适用范围：刺孢小单孢菌绛红变种、紫色小单孢菌（绛红小单孢菌）、白黄链霉菌、白色链霉菌、抗生链霉菌、双重轮丝链霉菌、产色链霉菌、烬灰链霉菌、天蓝色链霉菌、灭蚊链霉菌、红霉素链霉菌、青色链霉菌、球孢链霉菌、浅灰链霉菌、灰色链霉菌、吸水链霉菌、淡紫灰链霉菌、黄色长孢链霉菌、藤黄色链霉菌、细黄链霉菌、黑化链霉菌、玫瑰色链霉菌、华美链霉菌、嗜热链霉菌、委内瑞拉链霉菌、紫色直丝链霉菌、紫色链霉菌、绿色链霉菌 13. Wort Agar （麦芽汁琼脂） Dilute the world (without hop) to 12 Brix. Add 15g agar into 1000ml of the diluted word..Melt the agar by heating, then distribute the medium into tubes. Autoclave at 110 for 30 minutes. (将发酵啤酒的原料（未加酒花），稀释至12柏林，加琼脂15克，溶化后分装。15磅灭菌30分钟。) 适用范围：克鲁斯假丝酵母、郎比可假丝酵母、解脂假丝酵母、马其顿假丝酵母、拟热带假丝酵母、粗壮假丝酵母、皱褶假丝酵母、热带假丝酵母、产朊假丝酵母、阿舒假囊酵母、白地霉、果香地霉、地霉属、异常汉逊酵母、异常汉逊酵母变种、阿拉伯糖醇汉逊酵母、施氏汉逊酵母、菅囊酵母、伯顿毕赤酵母、膜醭毕赤酵母、粘红酵母、小红酵母、胶红酵母、深红酵母、卡尔斯伯酵母、酿酒酵母、椰园酿酒酵母、发酵性酵母、洛格酵母、罗斯酵母、鲁氏酵母多形鲁氏酵母、威尔酵母、酵母、路德类酵母、栗酒裂殖酵母、掷抱酵母、贝雷丝孢酵母、皮状丝孢酵母、糙孢曲霉、无壳曲霉、鲜橙曲霉、红曲霉、紫红曲霉、红色红曲霉、红曲霉菌 14. Potato Dextrose Agar PDA (马铃薯、葡萄糖琼脂) Potato extract (马铃薯汁) 1000ml Dextrose(glucose) (葡萄糖) 20g Agar (琼脂) 20g [Note]: Potato extract: Slice potatoes thin. Add 1000ml of water, immediately to prevent Oxidtion of juice and boil until soft. (approximately 30min.). Filter through cotton-cloth. Autoclave at 115℃ for 20 minutes.（注：取去皮马铃薯200克，切成小块，加水1000毫升煮沸30分钟，滤去马铃薯块，将滤液补足至1000毫升，加葡萄糖20克，琼脂15克，溶化后分装，15磅灭菌30分钟。） 适用范围：酵米面假单胞菌（酵米面黄杆菌）、白色链霉菌、烬灰链霉菌、青色链霉 菌、球孢链霉菌、灰色链霉菌、龟裂链霉菌、伞枝梨头霉、雅致放射毛霉、棒曲霉、 米曲霉、出芽短梗霉、白僵霉、灰葡萄孢、顶头孢霉、巴西毛壳、长刺毛壳、橄榄包 毛壳、反曲毛壳、琥珀毛壳、圆酵毛壳、束状刺盘孢、新月弯孢霉、奇异翅孢壳、地 生翅孢壳、串珠镰孢、尖镰孢、盘长孢菌（鲁保一号）、银白杨盘长孢、玉蜀黍长蠕孢、深黄被孢霉、小被孢霉、多头被孢、拉曼被孢霉、葡萄色被孢霉、生香毛霉、卷枝毛霉、两型孢毛霉、直立毛霉、球孢毛霉、丝球毛霉、大毛霉、多型孢毛霉、总状毛霉、鲁氏毛霉、五通桥毛霉、黑球漆斑菌、露湿漆斑菌、玫烟色拟青霉、棉铃虫拟青霉、拟青霉、球形阜孢、白腐菌、少根根霉、华根霉、科恩根霉、戴尔根霉、日本根霉、爪哇根霉、米根霉、点头根霉、绿穂霉、簇孢匍柄霉、雅致枝霉、康宁木霉、绿色木霉、黄萎轮枝孢、大丽花轮枝孢 15. Czapek′s Agar（察氏琼脂） Sucrose （蔗糖） 30g NaNO3 3g MgSO4.7H2O 0.5g KCl 0.5g FeSO4.7H2O 0.01g K2HPO4 1g Agar (琼脂) 13g Distilled water (蒸馏水) 1000ml pH 7.2 适用范围：红霉素链霉菌、洋葱曲霉、金头曲霉、泡盛曲霉、泡盛曲霉烟色变种、亮白曲霉、炭黑曲霉、鹿皮色曲霉、棒曲霉、无花果曲霉、黄柄曲霉、黄曲霉、烟曲霉、构巢曲霉、黑曲霉、赭曲霉、米曲霉、寄生曲霉、酱油曲霉、土曲霉、宇佐美曲霉、焦曲霉、杂色曲霉、温特曲霉、阿达青霉、产黄青霉、黄绿青霉、桔青霉、顶青霉、园弧青霉、绳状青霉、灰黄青霉、岛青霉、淡紫青霉、特异青霉、赭绿青霉、产紫青霉、娄地青霉、小刺青霉、荨麻青霉 16. Concentrated Sucrose Czapek′s (浓糖察氏琼脂) Sucrose (蔗糖) 200g NaNO3 3g MgSO4.7H2O 0.5g KCl 0.5g FeSO4.4H2O 0.01g K2HPO4 1g Agar (琼脂) 13g Distilled water (蒸馏水) 1000ml 适用范围：谢瓦曲霉 17. Synthetic Potato Medium (综合马铃薯培养基) 20%Potato extract (马铃薯汁) 1L Glucose (葡萄糖) 20g KH2PO4 3g MgSO4.7H2O 1.5g Vitanib B1 (硫胺素) Trace (微量) Agar (琼脂) 15g pH 6 适用范围：米曲霉、酱油曲霉、栖土曲霉、宇佐美曲霉、出芽短梗霉、白僵霉、灰葡萄孢、顶头孢霉、巴西毛客、长刺毛壳、 球毛壳、橄榄包毛壳、反曲毛壳、琥珀毛壳、圆酵毛壳、多主枝孢、束状刺盘孢、葫芦科刺盘孢、新月弯孢霉、奇异翅孢壳、地生翅孢壳、串珠镰孢、蚀脉镰孢霉、盘长孢菌（鲁保一号）、银白杨盘长孢、瓶毛壳、金龟子绿僵菌、生香毛霉、卷枝毛霉、两型孢毛霉、直立毛霉、球孢毛霉、丝球毛霉、大毛霉、多型孢毛霉、总状毛霉、鲁氏毛霉、五通桥毛霉、黑球漆斑菌、露湿漆斑菌、玫烟色拟青霉、棉铃虫拟青霉、淡紫色拟青霉、拟青霉、白腐菌、少根根霉、根霉属的菌、华根霉、科恩根霉、戴尔根霉、台湾根霉、日本根霉、爪哇根霉、黑根霉、雪白根霉、少根根霉、米根霉、点头根霉、三宝垄根霉、根霉属的种、绿穂霉、康宁木霉、木素木霉、拟康氏木霉、木霉、粉红木霉、绿色木霉、黄萎轮枝孢、大丽花轮枝孢、双孢蘑菇、大肥菇、发光假蜜环菌、木耳、皱木耳、毛木耳、大秃马勃、长根菇、金针菇、棘托竹荪、短裙竹荪、短裙竹荪东北变种（暂定）、长裙竹荪、白鬼笔、无裙竹荪、宽鳞大孔菌、凸圆灵芝（白芝）、紫芝、灵芝（红芝，赤芝，日本灵芝，韩国灵芝）、灰树花、猴头菌、香菇、粗毛斗菇、洁丽香菇、豹皮菇、玉蕈、真姬菇、安络小皮伞菌、鬼毛针、黑柄皮伞菌、尖顶羊肚菌、圆锥羊肚菌、高羊肚菌、黑脉羊肚菌、羊肚菌、滑菇、光帽黄伞、尖鳞黄伞、金顶側耳、榆黄磨、金顶磨、白黄側耳、紫孢平菇、姬菇、裂皮側耳、拟囊体側耳（鲍鱼菇）、真线側耳短孢变种、真线側耳、扇形平菇、扁形平菇、佛罗里达无孢子側耳、佛罗里达側耳、蚝菇、糙皮側耳、平菇、贝形側耳、鹰翅菌、肺形側耳、柳树菌、树窝、扁柄側耳、美味側耳、紫孢側耳、粉褶側耳、粉红側耳、凤尾菇、漏斗状側耳、榆干側耳、大榆磨、側耳、茯苓、黑菇、烟色红菇、裂褶菌、鸡纵、金耳、黄木耳、银耳、白木耳、紫晶口磨、银丝菇、丝盖小包脚菇、草菇 18. Corn Meal Medium (玉米粉培养基) Mix corn meal with water [20:100(W/V)] well . Distribute the mixture into 100ml bottles , each bottle contains about 30ml of the mixture . Autoclave at 121℃ for 30minutes . (20%玉米粉装瓶，15磅灭菌30分钟。) 19. Filter Paper Medium （滤纸培养基） （NH4）2SO4 1g KH2PO4 1g MgSO4.7H2O 0.7g NaCl 0.5g Distilled water （蒸馏水） 1000ml A strip of filter paper （滤纸条） 6ⅹ1cm （厘米） 20. Soybean Cake Meal Agar (黄豆饼粉琼脂) 10% infusion of soybean cake meal (10%黄豆饼粉浸汁) 1000ml Glucose (葡萄糖) 10g NaCl 2.5g 摘自：http://hi.baidu.com/silencefen/b ... 1d040a7bec2cae.html |
2楼2009-10-15 13:09:58
dingqian03
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司马诸葛(金币+5,VIP+0):真的是很全啊 10-15 14:42
司马诸葛(金币+5,VIP+0):真的是很全啊 10-15 14:42
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21. Sawdust?? Wheat-bran Medium ???м????????????? Sawdust of broad leaf tree 75g Wheat bran 25g Preparation??Mix these two ingredients well . Add appropriate water and blend (the amount of water added may be becided by kneading with fingers ). Fill the mixture into broad mouth of bottles . Stick a hole on the filling, put the cotton pulg in bottle mouth . ?????????????????м 75?? ?????????25?? ????????????????????????????????????????????????????????????С??????????????20?????1С??? 22. Pine Block or Pine Sowdust Medium ????????????м???????? 1. Cut 1021 cm pine biocks and immerse them in 1-2% sucrose solution . Alow the biocks fully absorbing suger solution .Fill the biocks into broad month of bottle . Park the bottle with paper . Autoclave at 20 lbs for 1 hr . (??10??2??1????????????????1-2%????????С?????????????????????????????????20?????1С???) 2. Pine Sawdust Medium Pine Sawdust (???м) 75% Rice bran (???) 23-24% Socrose (????) 1-2% Water (appropriate)? ???????? Autoclave at 20 lbs for 1 hr.(20?????1С??? 23. Rice Straw. Dung Medium (???????????????) Dried horse dung (or cow dung) ???????????? 65% Died straw ???????? 35% Urea ??????? 0.5% Calcium superhosphate ??????????0.7% Gypsum powder??????? 1% Ammonium sulphate ??????泥? 0.3% [Note]: Add appropriate water, pile them up and cause them fermentation. ??????????ж??????? 24. Straw Rice Bran Medium ?????????????????? Dried straw ???????? 75-80% Rice bran ??????? 20-25% [Note]??Select no mouldy straw and cut them about 3 cm in length. Immerse them in fresh water for 12 hr. Allow the straw fully (the amount of water added may be decided by kneading with fingers. It is right when a drop of water appeared between fingers). ( [?]?????ù??????г???3?????С?Σ??????????12С???????????????????????????????????????????????????????) 25. Rice Medium (?????????) Fill 10g of rice into a 50ml of Erlenmeyer flask. Add 20-30ml water. Autoclave at 121?? for 30 minutes (or fill 2g of rice into a 15??150mm tube). Add 6 ml of water. Autoclave at 121?? for 30 minutes. (?????10?????50?????????У??20-30??????15?????30???????????????2??????15??150????????У????6????????15?????30?????) 26. Filter Paper Medium (???????????) (NH4)2SO4 1g KH2PO4 1g ???? MgSO4.7H2O 0.5g Yeast Extract (?????) 0.1g Distilled Water (?????) 1000ml Strip of filter paper 1??7 cm (????? 1??7 ????) [Note]??Siddolve the above ingredients. Distribute the liqid medium into tubes (each tube contains 1ml of the broth). Autoclave at 121?? for 30 minutes. Then put one or two sterilized strips of filter paper into a tube aseptically. (??????????????????????????????1??????15?????30????????????????????1????2???????????м?????á?) 27. Yeast Extract-Medium Extract Agar I (?????????????) Yeast extract (?????) 10g Mall extract (?????) 10g Glucose (??????) 4g Distilled water (?????) 1000ml Agar (???) 20g pH 7.0 ???÷?Χ????ù????ù?? 28. Yeast Extract Peptone ?????????????????? Yeast extract ??????? 1g Multi-peptone????????? 2g Beef extract ?????? 1g Glucose ????????? 10g Agar ??????? 20g Distilled water ????????? 1L pH 7.0 29. Glucose Yeast Extract Starch Agar ??????????????????????? Glucose ????????? 10g Yeast extract ??????? 10g Starch ??????? 10g NaCl 5g CaCO3 3g Agar ??????? 20g Distilled water ????????? 1L pH 7.0 30. Wheat Bran Medium ????????????? Wheat bran ??????? 36g ??NH4??2HPO4 10g K2HPO4 0.2g MgSO4??7H2O 0.1g Agar ??????? 20g Distilled water ????????? 1L pH 7.0 31. Yeast Sporulation Medium (???????????????????????) (1) Kleyn Medium KH2PO4 0.012% K2HPO4 0.02% Sodium acetate (??????) 0.5% Glucose (??????) 0.062% NaCl 0.062% Peptone (??????) 0.25% Biotin (??????) 2??g% Agar (???) 2% Trace element solution (???????) 1ml/100ml [Note]: Trace element solution ???????????????? MgSO4.7H2O 0.4%, NaCl 0.4%, CuSO4.5H2O 0.002%, MnSO4.4H2O 0.2%, FeSO4.4H2O 0.2% (2) Gorodkowa Medium Glucose (??????) 0.1% Peptone (??????) 1% NaCl 0.5% Agar (???) 2% (3) McClary Medium Glucose (??????) 0.1% KCl 0.18% Yeast juice (????) 0.25% Sodium actate (??????) 0.82% Agar (???) 1.5% 32. BPY Medium (BPY ??????) Beef extract (????) 5g Prptone (??????) 10g NaCl 5g Yeast extract (?????) 5g Glucose (??????) 5g Agar (???) 1-2% Distilled (?????) 1000ml pH 7.0 ???÷?Χ????????????????????????????? 33. LB Medium (LB ??????) Yeast extact (?????) 5g Peptone (??????) 10g NaCl 10g Agar (???) 1-2% Distilled water (?????) 1000ml pH 7.0 ???÷?Χ????????????????? 34. Penssay Broth (Penssay ????) Beef extract (????) 1.5g Yeast extract (?????) 1.5g Tryptone (???????) 5g NaCl 3.5g Glucose (??????) 1g K2HPO4.3H2O 4.8g Distilled water (?????) 1000ml pH 7.2 35. Blond Agar (??????????) Peptone (??????) 10g Glucose (??????) 2g NaCl 5g NaH2PO4 2.5g Sodium citrate (????????) 5g Brain infusion (????) 800ml Pig heart infusion (?????) 200ml Sleep Blood (???) 10% pH 7.2 [Note]??Preparation of pig brain and pig heart infusion?? ??1?? Smash up 100g fresh pig heart ??without fat and blood vessel??.Add distilled water up to 1000ml; ??2?? Cut 200g fresh pig brain (without blood vessel). Add distilled water up to 1000ml; ??3?? Keep the two infusions at 50?? for 1 hr , then boil for 15 minutes; ??4?? Cool and filtrate through gauze . [?]?????????????????1??????????????????????????100??????????1000????????2?????????????????200??????????1000????????3??50?汣??1С????????15???????4??????????????????á? 36. Cellulose??decomposing Bacteria Synthetic Medium (???????????????) NaCl 6g MgSO4.7H2O 0.1g KH2PO4 0.5g CaCl2 0.1g K2HPO4 2.0g Yeast extract (?????) 1.0g (NH4)2SO4 2.0g Distilled water (?????) 1000ml Filter paper strip: one strip (?????????) pH 7.0-7.4 [Note]: So;id medium for cellulose-decomposing bacteria: Carboxymethyl Cellulose 2% Agar 2%. ??б????????????????2%?????2%??????????? CMCNa ?? 37. Clostridium Pasreurianum Synthetic Medium ????????????????????????? Glucose ????????? 1% MnSO4 0.001% KH2PO4 0.05% FeSO4 0.001% K2HPO4 0.05% Yeast extract??????? 0.1% MgSO4.7H2O 0.02% Peptone (??????) 0.01% NaCl 0.001% CaCO3 0.5% Distille water (?????) 100% pH 7.0 38. Yeast Extract-Medium Extract Agar ?? (????????????? ??) Yeast extract ??????? 4g Mall extract ??????? 10g Glucose ????????? 4g Agar ??????? 17g Distilled water ?????? 1000ml pH 7.3 39. Oatemeal Agar ???????????? Oatemeal??infusion??[???????] 20g Agar ??????? 18% Trace element solution ?????????????1ml Distilled water up to ???????????????1000?????? 1000ml pH 7.2 [Note]??Trace element solution (?????????)?? FeSO4.7H2O 0.1g MnCl2.4H2O 0.1g ZnSO4.7H2O 0.1g Distilled water (?????) 100ml 40. Calcium Malate Agar (?????????) Calium malate (??????) 10g K2HPO4 0.5g NH4Cl 0.5g Glycerol (????) 10g Agar (???) 15g Distilled water (?????) 1000ml 41. Thiobacillus Ferrooxidans Medium (??????????????????) (NH4)2SO4 0.15g KH2PO40.05g KCl 0.05g MgSO4.7H2O 0.5g Ca(NO3)2.4H2O 0.01g FeSO4.7H2O 50g Distilled water (?????) 1000ml pH 2.0 42. Thiobacillus Thiooxidans Medium (????????????????) (NH4)2SO4 0.3g KH2PO4 3-4g CaCl2 (anhydrous) [???] 0.25g MgSO4.7H2O 0.5g FeSO4.7H2O 0.001g Distilled water (?????) 1000ml pH 3.5-4 43. Plasma Substitute Medium (???????????) Sucrose (????) 12.5-15% Peptone (??????) 0.5% KH2PO4 0.03% Na2HPO4.12H2O 0.14% pH 7.0-7.2 44. Skim Milk Medium (????????????) ATCC 183 ?????? 100g ?????? 1000ml ???÷?Χ????????????????????????????? 45. Pea Agar (?????) Glucose (??????) 1% Peptone (??????) 0.5% NaCl 0.5% Agar (???) 2.0% Pea infusion (counted by NH2) [?????(??NH2??)] 12-14 mg?M100 pH (before sterilization) [???] 7.2-7.5 [Note]: Preparetion of pea infusion (??????????) : Preparetion 900g of pea. Put them into a 5L bottle, and add 3L of tap water. Adjust pH to 4.3-4.5 by using of sulphuric acid. Add 30ml of chloroform for antisepsis. Plug with rubber stopper and bind up the bottle mouth with gauge in order to avoid that the stopper fall off due to gas pressure. Place the bottle at 37?? for 7-10 days. Filter the infusion, then boil the filtrate to escape the chloroform. Filter again Distribution and autoclave at 120?? for 30 minutes. Store at cool contition . (???????900???????5?????????У????????3?????????????pH??4.3-4.5????????30???????????????????????????????????????????37???????7-10?????????????????????20?????????????????????????????????????????????120??30????????????????á?) The standard quality of pea infusion as following (????????????)?? Aminonitrogen????????: 160-200 mg?M100ml Soluble phosphoruse???????: 400-600 ??g?Mml Color?????: auratus,transparent ??????????? ????http://hi.baidu.com/silencefen/b ... 1d040a7bec2cae.html |
3楼2009-10-15 13:11:13
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4楼2009-10-15 13:21:44
