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zlgong

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[交流] 求助关于Job's Plots的做法。

请教如何用紫外方法得到Job's Plot图。具体如何操作，比如纵坐标是代表甚么意思，横坐标是否都是浓度比值。我们是非专业学生，遇到这个不知道如何处理，请高手赐教。不胜感激

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1楼 2009-10-13 16:17:07

已阅回复此楼关注TA 给TA发消息送TA红花 TA的回帖

vjyong

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zlgong(金币+2,VIP+0):说的太笼统了，我只想知道纵，横坐标代表什么？怎么做就可以了。希望您能指点一下 10-13 18:10

A Job plot is used to determine the stoichiometry of a binding event. It is also known as the Method of Continuous Variation. In this method, the total molar concentration of the two binding partners (e.g. a protein and ligand) are held constant, but their mole fractions are varied. An observable that is proportional to complex formation (such as absorption signal or enzymatic activity) is plotted against the mole fractions of these two components. The maximum on the plot corresponds to the stoichiometry of the two species[1] if sufficiently high concentrations are used. This method is named after P. Job, who first introduced this methodology in 1928[2].
[edit] References

1. ^ Huang, C.Y. Determination of Binding Stoichiometry by the Continuous Variation Method: The Job Plot. Methods in Enzymology (1982) 87, 509-525.
2. ^ Job, P. Ann. Chim. (1928) 9, 113-203