24小时热门版块排行榜    

查看: 461  |  回复: 0

biolite123

铁虫 (小有名气)

[交流] 百萤 核酸检测的好帮手----DNA检测染料 PUR-1

一、百萤 DNA检测染料 PUR-1简介

在没有核酸的情况下,PUR-1基本不发荧光。在存在DNA或RNA的情况下,其荧光显着增强。在存在RNA的情况下,PUR-1在〜478 nm处具有荧光,荧光量子产率为〜40%。PUR-1染料在488 nm处可激发(在460 nm处有大激发),并且在存在核酸的情况下发出470至550 nm之间的荧光,在478 nm处具有大发射。该染料可与流式细胞仪结合用于分析血源性寄生虫感染。

二、百萤 DNA检测染料 PUR-1参数

分子量     437.30
溶   剂    DMSO
存储条件   在-15℃以下保存,避光防潮
保质期     12个月
激发波长(nm)  459
发射波长(nm)   478

三、百萤 DNA检测染料 PUR-1参考文献

Activation of Mitochondrial Uncoupling Protein 4 and ATP-Sensitive Potassium Channel Cumulatively Decreases Superoxide Production in Insect Mitochondria
Authors: Slocinska M, Rosinski G, Jarmuszkiewicz W.
Journal: Protein Pept Lett (2016): 63
 
Rasagiline prevents cyclosporine A-sensitive superoxide flashes induced by PK11195, the initial signal of mitochondrial membrane permeabilization and apoptosis
Authors: Wu Y, Shamoto-Nagai M, Maruyama W, Osawa T, Naoi M.
Journal: J Neural Transm (Vienna) (2016): 491
 
Mitochondrial disease-related mutations at the cytochrome b-iron-sulfur protein (ISP) interface: Molecular effects on the large-scale motion of ISP and superoxide generation studied in Rhodobacter capsulatus cytochrome bc1
Authors: Ekiert R, Borek A, Kuleta P, Czernek J, Osyczka A.
Journal: Biochim Biophys Acta (2016): 1102
 
Oleuropein Prevents Neuronal Death, Mitigates Mitochondrial Superoxide Production and Modulates Autophagy in a Dopaminergic Cellular Model
Authors: Achour I, Arel-Dubeau AM, Renaud J, Legr and M, Attard E, Germain M, Martinoli MG.
Journal: Int J Mol Sci (2016): 1293
 
Manganese-superoxide dismutase (Mn-SOD) overexpression is a common event in colorectal cancers with mitochondrial microsatellite instability
Authors: Govatati S, Malempati S, Saradamma B, Divyamaanasa D, Naidu BP, Bramhachari PV, Narayana N, Shivaji S, Bhanoori M, Tamanam RR, Rao PS, Nallanchakravarthula V.
Journal: Tumour Biol. (2016)
 
Production of superoxide/hydrogen peroxide by the mitochondrial 2-oxoadipate dehydrogenase complex
Authors: Goncalves RL, Bunik VI, Br and MD., undefined
Journal: Free Radic Biol Med (2016): 247
 
A Unifying Mechanism for Mitochondrial Superoxide Production during Ischemia-Reperfusion Injury
Authors: Chouchani ET, Pell VR, James AM, Work LM, Saeb-Parsy K, Frezza C, Krieg T, Murphy MP.
Journal: Cell Metab (2016): 254
 
Rotenone-stimulated superoxide release from mitochondrial complex I acutely augments L-type Ca2+ current in A7r5 aortic smooth muscle cells
Authors: Ochi R, Dhagia V, Lakhkar A, Patel D, Wolin MS, Gupte SA.
Journal: Am J Physiol Heart Circ Physiol (2016): H1118
 
Recombinant Mitochondrial Manganese Containing Superoxide Dismutase Protects Against Ochratoxin A-Induced Nephrotoxicity
Authors: Ciarcia R, Damiano S, Squillacioti C, Mirabella N, Pagnini U, Florio A, Severino L, Capasso G, Borrelli A, Mancini A, Boffo S, Romano G, Giordano A, Florio S.
Journal: J Cell Biochem (2016): 1352
 
Mitochondrial Calpain-1 Disrupts ATP Synthase and Induces Superoxide Generation in Type 1 Diabetic Hearts: A Novel Mechanism Contributing to Diabetic Cardiomyopathy
Authors: Ni R, Zheng D, Xiong S, Hill DJ, Sun T, Gardiner RB, Fan GC, Lu Y, Abel ED, Greer PA, Peng T.
Journal: Diabetes (2016): 255
回复此楼
百萤,色彩让科学更生动
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
相关版块跳转 我要订阅楼主 biolite123 的主题更新
普通表情 高级回复 (可上传附件)
最具人气热帖推荐 [查看全部] 作者 回/看 最后发表
[公派出国] 售SCI一区文章,我:8 O5 51O 54,科目齐全 +3 5lbyq5wrhb 2026-02-07 3/150 2026-02-08 03:05 by vs90ilomwc
[考博] 售SCI一区文章,我:8 O5 51O 54,科目齐全 +3 5lbyq5wrhb 2026-02-07 3/150 2026-02-08 02:52 by vs90ilomwc
[论文投稿] 售SCI一区文章,我:8 O5 51O 54,科目齐全 +3 3rkserf6qr 2026-02-07 4/200 2026-02-08 02:45 by vs90ilomwc
[硕博家园] 售SCI一区文章,我:8 O5 51O 54,科目齐全 +5 2h7du0nuhk 2026-02-07 5/250 2026-02-08 02:27 by vs90ilomwc
[考博] 售SCI一区文章,我:8 O5 51O 54,科目齐全 +5 2h7du0nuhk 2026-02-07 5/250 2026-02-08 02:25 by vs90ilomwc
[硕博家园] 售SCI一区文章,我:8 O5 51O 54,科目齐全 +4 2h7du0nuhk 2026-02-07 5/250 2026-02-08 02:12 by vs90ilomwc
[考博] 售SCI一区文章,我:8 O5 51O 54,科目齐全 +4 2h7du0nuhk 2026-02-07 6/300 2026-02-08 02:07 by vs90ilomwc
[教师之家] 售SCI一区文章,我:8 O5 51O 54,科目齐全 +4 2h7du0nuhk 2026-02-07 6/300 2026-02-08 02:05 by vs90ilomwc
[找工作] 售SCI一区文章,我:8 O5 51O 54,科目齐全 +4 2h7du0nuhk 2026-02-07 6/300 2026-02-08 01:46 by vs90ilomwc
[公派出国] 售SCI一区文章,我:8 O5 51O 54,科目齐全 +4 2h7du0nuhk 2026-02-07 7/350 2026-02-08 01:45 by vs90ilomwc
[考博] 售SCI一区文章,我:8 O5 51O 54,科目齐全 +4 2h7du0nuhk 2026-02-07 7/350 2026-02-08 01:32 by vs90ilomwc
[教师之家] 售SCI一区文章,我:8 O5 51O 54,科目齐全 +4 2h7du0nuhk 2026-02-07 7/350 2026-02-08 01:26 by vs90ilomwc
[硕博家园] 售SCI一区文章,我:8 O5 51O 54,科目齐全 +4 2h7du0nuhk 2026-02-07 7/350 2026-02-08 01:12 by vs90ilomwc
[教师之家] 有院领导为了换新车,用横向课题经费买了俩车 +7 瞬息宇宙 2026-02-04 7/350 2026-02-07 21:47 by tfang
[有机交流] 酰胺脱乙酰基 10+5 chibby 2026-02-03 12/600 2026-02-07 19:29 by 江东闲人
[基金申请] 同年申请2项不同项目,第1个项目里不写第2个项目的信息,可以吗 +4 hitsdu 2026-02-06 4/200 2026-02-07 13:07 by jurkat.1640
[基金申请] 有时候真觉得大城市人没有县城人甚至个体户幸福 +9 苏东坡二世 2026-02-04 10/500 2026-02-07 12:37 by 小毛球
[考博] 天津大学招2026.09的博士生,欢迎大家推荐交流(博导是本人) +4 a793625982 2026-02-05 5/250 2026-02-07 10:57 by a793625982
[基金申请] 面上项目申报 +3 Tide man 2026-02-01 3/150 2026-02-05 22:56 by god_tian
[硕博家园] 博士延得我,科研能力直往上蹿 +7 偏振片 2026-02-02 7/350 2026-02-04 17:36 by 陈氏帝国
信息提示
请填处理意见