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shuaili518至尊木虫 (著名写手)
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【求助】Dopamine Hydrochloride的USP31标准
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本人急需Dopamine Hydrochloride的USP31标准，坛内高手能否给予帮助？ [ Last edited by jw227 on 2009-10-9 at 18:08 ] |
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jinlin
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2楼2009-10-09 18:22:41
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Dopamine Hydrochloride C8H11NO2·HCl 189.64 1,2-Benzenediol, 4-(2-aminoethyl)-, hydrochloride. 4-(2-Aminoethyl)pyrocatechol hydrochloride [62-31-7]. » Dopamine Hydrochloride contains not less than 98.0 percent and not more than 102.0 percent of C8H11NO2·HCl, calculated on the dried basis. Packaging and storage— Preserve in tight containers. Store at room temperature. USP Reference standards 11— USP Dopamine Hydrochloride RS. Clarity and color of solution— A solution of 400 mg in 10 mL of sodium bisulfite solution (1 in 1000) is clear and colorless or practically colorless. Identification— A: Infrared Absorption 197K. B: Ultraviolet Absorption 197U— Solution: 40 µg per mL. Medium: sodium bisulfite in water (1 in 1000). C: It responds to the tests for Chloride 191. pH 791: between 3.0 and 5.5, in a solution (1 in 25). Loss on drying 731— Dry it at 105 for 2 hours: it loses not more than 0.5% of its weight. Residue on ignition 281: not more than 0.1%. Heavy metals, Method I 231— Dissolve 1 g in 25 mL of water: the limit is 0.002%. Sulfate 221— Dissolve 500 mg in 40 mL of water: any turbidity produced is not more than that produced in a solution containing 0.10 mL of 0.020 N sulfuric acid. Readily carbonizable substances 271— Dissolve 100 mg in 5 mL of sulfuric acid TS: the solution has no more color than Matching Fluid A. Chromatographic purity— Standard solution and Standard dilutions— Prepare a solution of USP Dopamine Hydrochloride RS in methanol to contain 30 mg per mL (Standard solution). Prepare a series of dilutions of the Standard solution in methanol to contain 0.6 mg, 0.3 mg, and 0.15 mg per mL (Standard dilutions), corresponding to 2.0%, 1.0%, and 0.5% of impurities, respectively. Test solution— Transfer 150 mg of Dopamine Hydrochloride to a 5-mL volumetric flask, dilute with methanol to volume, and mix. Procedure— In a suitable chromatographic chamber arranged for thin-layer chromatography (see Chromatography 621), place a solvent system consisting of 13 volumes of chloroform, 9 volumes of methanol, and 4 volumes of dilute glacial acetic acid (3 in 10). Line the chamber with filter paper, and allow to equilibrate. Apply 10-µL portions of the Standard solution, the Standard dilutions, and the Test solution to a 20- × 20-cm chromatographic plate coated with a 0.25-mm layer of chromatographic silica gel mixture. Develop the chromatogram until the solvent front has moved about 15 cm. Remove the plate from the developing chamber, allow to dry at room temperature for several minutes, and spray evenly with a freshly prepared mixture containing equal volumes of ferric chloride solution (1 in 10) and potassium ferricyanide solution (1 in 20). [Dopamine and its related impurities appear as blue spots under visible light.] The Test solution exhibits its principal spot at an RF value corresponding to that of the Standard solution and not more than three secondary spots. Estimate the concentration of any secondary spots exhibited by the Test solution by comparison with the Standard dilutions: the sum of the impurities is not greater than 1.0%. Assay— Dissolve about 75 mg of Dopamine Hydrochloride, accurately weighed, in 5 mL of formic acid, add 25 mL of acetic anhydride, and mix. Titrate with 0.1 N perchloric acid VS, determining the endpoint potentiometrically. Perform a blank determination, and make any necessary correction. Each mL of 0.1 N perchloric acid is equivalent to 18.96 mg of C8H11NO2·HCl. Auxiliary Information— Staff Liaison : Sujatha Ramakrishna, Ph.D., Scientist Expert Committee : (MDCV05) Monograph Development-Cardiovascular USP31–NF26 Page 2012 Pharmacopeial Forum : Volume No. 29(5) Page 1469 Phone Number : 1-301-816-8349 |
3楼2009-10-09 19:19:41
shuaili518
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4楼2009-10-09 22:20:38
