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Glycerol fatty acid Diethanolamide the determination has yet to see the relevant literature. Determination of the main reference of China GB / T 22328-2008 "plant and animal oils and fats 1 - monoglycerides and free glycerol content in" and "bio-diesel fuel glycerin content, the determination of total glycerol content." However, fatty acid amide composition and two ethanol bio-diesel plants and animals are different Single-monoglyceride, the above methods of glycerol fatty acid Diethanolamide content in the applicability has yet to be inspected. In this paper, using the above method of fatty acids of this company Diethanolamide the determination of the glycerine content of the experimental results show that the national standard method is more suitable for fatty acid Diethanolamide the determination of glycerol. National Standard Method in the process of discovery, with the volume fraction of 5% acetic acid extraction, the emulsion phenomenon more obvious; and 6501 because no determination of amide content. Therefore, this paper, based on national standard method to do some changes and simplifies the determination of the steps. As well as the determination of the modified method of recovery, precision and linear correlation to be looked at, reliable and accurate
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