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【强烈推荐美国生物实验手册--网络版】
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强烈推荐美国生物实验手册www.lab-manual.com网络版 美国, 网络版, 手册, 生物, 实验 美国生物实验手册网络新版-- www.lab-manual.com -- 共十五章 Lab-Manual.Com 版权，仅供交流。 链接为： http://www.lab-manual.com/ --------------------------------------------------------------------------- 这里面有生物实验室的各种操作的详细步骤和问题解答， 英语描述，图文并茂，适合一般实验室实验的各种需要！ 网络版的目录 Laboratory Manual for Biomedical Research New Edition Chapter 1. General Lab Techniques Lab security and basic techniques Advanced lab skills Advanced lab skills (2) Chapter 2. Molecular Biology -- Molecular Separation Molecular Separation DNA Agarose Gel Electrophoresis RNA Agarose Gel Electrophoresis SDS-Page - Polyacrylamide gel electrophoresis Ion Exchange Chromatography Gel Filtration Chromatography Affinity chromatography Chapter 3. Molecular Biology -- DNA and RNA Nucleic acid methods (1) Nucleic acid methods (2) DNA isolation related protocols DNA Purification (glass milk vs electroelution) DNA, RNA Sequencing RNA Isolation and Purification DNA mutation detection by SSCP Preparation of DNA and RNA probes Southern blot hybridization Northern blot hybridization Loss of Heterozygosity (LOH) Gene knockout protocol SiRNA gene knockout Plasmid and its usefulness DNA library construction Microarray protocols. Basic knowledge of microarray. Introduction to Microarray. Microarray Dababases Troubleshooting Other Microarray Protocols (1,2) Gene transfection Gene therapy for cancer Molecular cloning Conditional gene transfection(Tet on/off) Chapter 4. Genetics Epigenetics protocols Mutagenesis protocols Single nucleotide polymorphisms (SNPs) Chapter 5. Molecular Biology --PCR Serials PCR,RT-PCR,Real time PCR etc. PCR (General Procedure) Recommended Reagent Concentrations Recommended Reaction Conditions Initial Conditions Temperature Cycling "Hot Start" PCR Asymmetric PCR for ssDNA Production Detecting Products Labelling PCR Products with Digoxigenin Cleaning PCR Products Sequencing PCR Products Cloning PCR Products AND ALWAYS REMEMBER PCR Primer Design Tools RT-PCR Real time PCR More PCR Protocols Online Video and Animation of PCR Mouse Genotyping by PCR PCR Based Molecular Cloning PCR Troubleshootings Chapter 6. Molecular Biology – Protein Protein methods Protein labeling techniques Protein sequencing Subcellular fractionations Western blot hybridization Western Blot Hybridization Center Western Blot Full Procedure (from Pierce) Western Blotting Procedure with Video Show Protocol 1: Western Blot Sample protein preparation Protein concentration assay Electrophoresis and blotting Blocking non-specific antigen Incubation with primary antibody Incubation with secondary antibody Substrate (ECL) incubation Restore a western blot using pierce stripping buffer Western blot related solutions and buffers Western blot handbook Western blot troubleshooting Protocol 2: Western Blot 2. Protocol 3: Western Blot 3. More online Western blot protocols (1) (2) Protein chips Kinase assay Methods for detecting protein phosphorylation Chapter 7. DNA Protein Interactions EMSA ChIP assay Chapter 8. Immunohistochemistry/immunology Immunology/Histology Preparing silanized (+plus) slides Microscopy Techniques Confocal microscope technique Electron microscopy HE staining DAPI & PI nucleic acid stain Hybridization in situ FISH Special cell & cell fraction stains Antibody purification Antibody storage and handling Conjugation of monoclonal antibodies Antigen retrieval Immunoperoxidase staining techniques Immunofluorohistochemistry Immunoprecipitation Histotechnology--technical methods FRQs for histochemistry Laser Capture Microdissection Elisa Chapter 9. Cellular Biology General cell culture protocols Chromosome karyotype Proliferation assays (MTT, BrdU, 3H-Thymidine incoporation) Cell cycle assay Migration assay Stem cell & related protocols Introduction What are the unique properties of all stem cells? What are embryonic stem cells? What are adult stem cells? Culture of Human Embryonic Stem Cells (hESC) Establishment and characterization of human embryonic stem cell line Rat stem cell isolation and culture Mouse stem cell isolation and culture Induction of Stem Cell Differentiation Cancer stem cells Video Data on Stem Cell Research (click to show) Apoptosis and related protocols Soft Agar Assay for Colony Formation Aorta ring assay GFP transfection Blood cell fractionation (white blood cell isolation) Endothelial cell isolation and culture protocols Flow cytometry (FCM) Chapter 10. Metabolism, GC/MS, NMR and Proteomics GC/MS Background Glucose metabolism and its related protocols PAS staining Deoxyribose procedure Ribose metabolism analysis Polysaccharide sequencing Lactate cleanup and derivative Amino acids Fatty acids Lipid protocols Cholesterol Bile acids Urea procedure Choline Incorporation Assay Isotope Ratio Mass Spectrometer (IRMS) Liquid chromatography / mass spectrometer (LC/MS) Proteomics NMR protocols and tutorials Chapter 11. Animal Experiments Blood sampling from animals Basic skills for animal experiments Anatomy and physiology of animals Cancer xenograft animal models Transgenic animal procedures Transgenic cancer models Animal models for depression-like and anxiety-like behavior Chapter 12. Worm: C. Elegans C. Elegans Protocols Chapter 13. HPLC and TLC HPLC protocols TLC Chapter 14. Buffers and Solutions in Lab. Commonly used solutions (1)--- 1 ) 2 ) 3 ) 4 ) 5 ) 6 ) Commonly used solutions (2)--- 1 ) 2 ) 3 ) Solutions for molecular cloning---1 ) 2 ) 3 ) 4 ) 5 ) 6 ) 7 ) 8 ) 9 ) Chapter 15. Other Resources Free eBooks at Library Online Cinema Online, Free Movies --(1) (2) (3) Progresses in Life Science Free eBooks in life science (1) Free eBooks in life science (2,3) Most Updated Biomedical Books Pathway databases Pathway Search Biomedical Job Opportunities Tools for Statistics Biological Educational Resources Genetics Education Resources of Medical Biochemistry Textbooks and Lab Manuals ----------------------------------------------------------------------------- 先看一个内容的例子： ----------------------------------------------------------- PCR Serials and Its Related Protocols PCR Serial and It's Related Protocols PCR (General Procedure) PCR Primer Design Tools RT-PCR Real time PCR More PCR Protocols Online Video and Animation of PCR Variants of PCR Regular PCR Procedure PCR Protocol and Its Product Processes Recommended Reagent Concentrations Recommended Reaction Conditions Initial Conditions Temperature Cycling "Hot Start" PCR Asymmetric PCR for ssDNA Production Detecting Products Labelling PCR Products with Digoxigenin Cleaning PCR Products Sequencing PCR Products Cloning PCR Products AND ALWAYS REMEMBER: Protocol for PCR using Taq DNA Polymerase Protocol for PCR with Taq DNA Polymerase. Avoiding Contamination. PCR allows the production of more than 10 million copies of a target DNA sequence from ... www.fermentas.com/techinfo/pcr/dnaamplprotocol.htm - General PCR Protocol Detailed PCR protocol from the web site of the Department of Biology, University of Michigan, USA. www.mcdb.lsa.umich.edu/labs/madd ... l_pcr_protocol.html Standard PCR However, efficient sequencing of dsDNA generated by normal PCR is possible using the modification to the SequenaseTM protocol published by Bachmann et al. ... www.mcb.uct.ac.za/pcrcond.htm PCR PROTOCOL PCR PROTOCOL FOR cDNA ARRAYS ON MEMBRANES. Purpose: to amplify insert DNA from purified plasmid DNA derived from bacterial. plasmid libraries. ... www.daf.jhmi.edu/microarray/protocols/protocol6.pdf Basic PCR Protocol Basic PCR Protocol. CGLab, 7/2002. 1). Wipe down the bench area with bleach and a new paper towel. 2). Take the PCR components out of the freezer to thaw ... www.sfsu.edu/~biology/cgl/media/PCR%20Protocol-Basic.pdf Long PCR Protocol Protocol and guidelines for choice of conditions for PCR of long sequences (10 kb or larger). From Genetics Dept., Harvard Medical School,Boston, MA, USA. arep.med.harvard.edu/labgc/estep/longPCR_protocol.html 20-mer Polymerase Chain Reaction Procedure (for MJ Research ... MJ Research thermal Cycler: 10-mer PCR for amplification of random genomic DNA fragments ... Edit (or choose a program if it has been set up) PCR Program. ... wheat.pw.usda.gov/~lazo/methods/lazo/pcrproto.html Single tube confirmation PCR protocol For characterization colonies of transformed clones of Saccharaomyces, from the web site of the Stanford Genome Technology Center, Palo Alto, CA, USA. www-sequence.stanford.edu/group/yeast_deletion_project/single_tube_protocol.html Protocol for PCR with Hot Start Taq DNA Polymerase Protocol for PCR with Hot Start Taq DNA Polymerase. How to Avoid Contamination. During PCR, usually more than 10 million copies of a template DNA can be ... www.fermentas.com/profiles/modif ... rotocolhotstart.pdf A Basic Polymerase Chain Reaction Protocol Here, a basic, straight-forward PCR protocol is. presented. Where appropriate, some of the choices for modifying this standard reaction ... www.idtdna.com/support/technical ... ic_PCR_Protocol.pdf PCR Reamplification Protocol PCR Reamplification for Inadequate or Failed Amplifications. Change your standard PCR protocol for the locus as follows:. decrease the number of cycles by ... genome-lab.ucdavis.edu/Protocols/pcr_tips/pcr_reamplification.htm Inverse PCR and Sequencing Protocol Inverse PCR and Sequencing Protocol on 5 Fly Preps. For recovery of sequences flanking XP elements. This protocol is an adaptation of ... flystocks.bio.indiana.edu/pdfs/Exel_links/5__fly_iPCR_XP_pub.pdf Enter your search terms Submit search form Web www.lab-manual.com ------------------------------------------------------------------------------------------------- PCR Reamplification for Inadequate or Failed Amplifications Change your standard PCR protocol for the locus as follows: decrease the number of cycles by 10 raise the annealing temperature by 2°C lower the final primer concentration by 0.1 uM lower the final MgCl2 concentration by 0.5 mM (but not lower than 1.0 mM) add 1.0 ul of the previous PCR reaction (cannot contain loading dye) n addition, attempt reamplification of the negative control(s) from the previous reaction to be sure the reamplification product is not contaminated may want to repeat the reamplification, especially in the case of rare alleles, to confirm there were no polymerase errors be wary of excess of homozygotes in results--may be a consequence of allelic drop-out *NOTE: this is a general protocol and may need adjustment per locus  |
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