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Joy_sumu

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[交流] 想请教一下MDA-MB-231细胞的转染经验

我用lipo2000转染siRNA到MDA-MB-231细胞中,不知道是哪个环节出了问题,收回来的细胞一直不多,导致下游提RNA做qPCR结果时好时坏。我同时养的另一个细胞系MCF-7就没有出现过这种情况,想请教一下有没有人做过类似实验,具体的铺板密度、转染时机、消化方式有什么需要注意的,还是说MDA-MB-231有一些特殊之处。十分感谢!!
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