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[求助]
求助根据波长、摩尔消光系数、激发态寿命等参数怎样计算激发一半分子所需光强
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| 题目：Suppose you have a fluorophore with a molar extinction of 104,000 M–1 cm–1 at 600 nm, and a lifetime of 4 ns. Assume there is no intersystem crossing or blinking. Calculate the light intensity in watts/cm2 needed to result in half of the molecule in the excited state. If the illuminated is 1 µm2 what is the needed power? Assume the objective transmits 100% of the light. |
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