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Post-Doctoral Research Associates
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Post-Doctoral Research Associate Position Summary: Post-doctoral research associate positions are available under the direction of Prof. David R. Gang in the Laboratory for Cellular Metabolism and Engineering at the Institute of Biological Chemistry, Washington State University. These positions will be part of an interdisciplinary research team working to apply a comparative functional genomics approach to identify and characterize genes and enzymes that play important roles in defining the growth and function of plant rhizomes. Several model and non-model species from across the plant kingdom, including advanced dicots and monocots, as well as more primitive angiosperms and other vascular plants, are being investigated. The post-docs involved in this project will be involved in production and analysis of gene expression profiles, identification of rhizome-specific genes, and analysis of gene function using a number of tools including: next-generation sequencing technology, knock-out of gene expression by RNAi-mediated gene silencing in source species, heterologous over-expression in alternative species, and biochemical characterization of specific proteins. Duties and Responsibilities of these post-doctoral scholars will include the following: • utilize next-generation sequencing technologies to produce deep coverage EST libraries • perform global gene expression profiling using modern approaches (next-generation sequencing) • produce protein samples for proteomic analysis from small amounts of tissue, including meristematic regions of developing rhizomes, and assist in interpretation of proteomics results • analyze EST sequence data to identify genes of interest • characterize the function of genes using modern genomics- and biochemical-based approaches and genetics where applicable • adapt current and develop new methods for plant transformation of model and non-model plant species • produce specific knockouts or over-expression constructs for genes that appear to have important roles in rhizome growth, development, and biology, and analyze the resulting plants. Minimum Qualifications: Ph.D. in biochemistry, genomics, or modern plant sciences by date of hire. Excellent oral and written communication skills. Ability to work well as a member of an integrated research team. Preferred Qualifications: Documented expertise (as demonstrated by relevant publications) in two or more of the following three areas: 1. A strong background in use of next-generation sequencing technology (454/Roche sequencing, Solexa/Illumina sequencing, SOLiD sequencing). 2. A strong background in modern biochemical analysis of protein function, including expression of proteins in heterologous systems and purification of proteins from native and heterologous systems, analysis of protein activity using modern detection techniques, such as yeast-two and one-hybrid approaches, promoter binding analysis, and enzyme activity assays. 3. A strong background in the successful genetic transformation of grasses and/or other monocots. Individuals with experience in characterization of gene function in meristematic regions, or with experience in bioinformatics (database and/or tool development) are especially encouraged to apply if they have expertise in at least two of the other areas listed above. To apply, please submit a cover letter, CV and the names and contact information for three references to: David R. Gang Institute of Biological Chemistry Clark 299 P.O. Box 646340 Pullman, WA 99164-6340 520-621-7154 gang@ag.arizona.edu Review of application materials will begin immediately. Washington State University is an EEO/AA Employer and Educator Post-Doctoral Research Associate Position Summary: A post-doctoral research associate position is available under the direction of Prof. David R. Gang in the Laboratory for Cellular Metabolism and Engineering at the Institute of Biological Chemistry, Washington State University. This position will be part of an interdisciplinary research team working to combine enzymology and structural biology to investigate the evolution of polyketide synthase-like (PKS) genes involved in production of medicinally important compounds related to curcumin and the gingerols from turmeric and ginger. The post-doc involved in this project will be involved in production of recombinant proteins and crystallization and structural characterization, as well as biochemical characterization of specific proteins. This individual will also assist with analysis of gene expression profiles and heterologous over-expression in alternative species. Duties and Responsibilities of this post-doctoral associate will include the following: • Determine the biochemical function of each PKS in ginger and turmeric. • Determine the structural basis for differential activity among the different PKSs. • Assist with characterization of expression patterns of all known polyketide synthase-like enzymes in ginger and turmeric and comparison of these expression patterns to metabolite production profiles. • Assist with production of transgenic plants (such as potatoes or tomatoes) that produce either gingerol or curcumin as a means of validating the mechanism of production for these compounds. Minimum Qualifications: Ph.D. in biochemistry, chemistry, chemical biology, structural biology or related field by date of hire. Excellent oral and written communication skills. Ability to work well as a member of an integrated research team. Preferred Qualifications: Documented expertise (as demonstrated by relevant publications) in the following areas: 1. Crystallization and/or structural determination of proteins. 2. Ligand docking. 3. Protein design. To apply, please submit a cover letter, CV and the names and contact information for three references to: David R. Gang Institute of Biological Chemistry Clark 299 P.O. Box 646340 Pullman, WA 99164-6340 520-621-7154 gang@ag.arizona.edu Review of application materials will begin immediately. Washington State University is an EEO/AA Employer and Educator Post-Doctoral Research Associate Position Summary: Post-doctoral research associate positions are available under the direction of Prof. David R. Gang in the Laboratory for Cellular Metabolism and Engineering at the Institute of Biological Chemistry, Washington State University. These positions will be part of an interdisciplinary and multi-institutional research team in the Center for Enhanced Biofuel Systems (CABS), a DOE funded EFRC program. Post-doctoral associates in the Gang lab will identify enzymes that play important roles in regulating and controlling flux and partitioning through the metabolic networks of single cell types: (A) glandular trichomes from several Solanum species and (B) the single celled Chlamydomonas. These proteins will then be reengineered in target biofuel species in efforts to alter allocation to specific pathways and lead to high yielding lines. Duties and Responsibilities of these post-doctoral scholars will include the following: • Investigate the role of phosphorylation and transcriptional regulation in controlling enzyme activity in all major branches of metabolism within glandular trichomes of Solanum species. • Compare detailed phosphoproteomics analysis of proteins isolated from Solanum trichomes collected at different stages of plant and leaf development to metabolite profiles and gene expression profiles (using microarrays/mRNA-seq and/or qRT-PCR for specific genes) from the same biological samples. • Identify enzymes that serve as targets for control of metabolism by protein phosphorylation, as well as potentially by transcriptional regulation, and which thereby may regulate partitioning between pathways in specific cell types. • Identify specific sites of phosphorylation. • Work with CABS partners to develop a flux model for metabolism in these cell types. • Evaluate potential regulatory proteins/enzymes in the target tissues of the target organisms for bioengineering (Camelina and Chlamydomonas) • Reengineer specific proteins that sit at key regulatory nodes in the pathways to either oil or specialty metabolite production in efforts to alter their regulation (such as by elimination of phosphorylation sites, changes in transcriptional control of expression, etc.) and evaluate the effects on metabolism in the target biofuel crops. Minimum Qualifications: Ph.D. in biochemistry, genomics, or modern plant sciences by date of hire. Excellent oral and written communication skills. Ability to work well as a member of an integrated research team. Preferred Qualifications: Documented expertise (as demonstrated by relevant publications) in two or more of the following three areas: 1. Experience in transformation and engineering of Solanum species. 2. Experience in engineering Chlamydomonas. 3. Experience in engineering modification to phosphorylation in proteins. To apply, please submit a cover letter, CV and the names and contact information for three references to: David R. Gang Institute of Biological Chemistry Clark 299 P.O. Box 646340 Pullman, WA 99164-6340 520-621-7154 gang@ag.arizona.edu Review of application materials will begin immediately. Washington State University is an EEO/AA Employer and Educator Post-Doctoral Research Associate Position Summary: Two post-doctoral research associate positions are available under the direction of Prof. David R. Gang in the Laboratory for Cellular Metabolism and Engineering at the Institute of Biological Chemistry, Washington State University. These positions will be part of an interdisciplinary and multi-institutional research team centered in the IBC. Post-doctoral associates in the Gang lab will work with other project team members to develop a kinetic model of metabolism in glandular trichomes of sweet basil and peppermint. They will identify enzymes that play important roles in regulating and controlling flux and partitioning through the metabolic network in this cell type. These proteins will then be reengineered and/or their expression will be altered in efforts to test the validity of the kinetic model. Duties and Responsibilities of these post-doctoral scholars will include the following: • Measure metabolite levels from glandular trichomes at specific developmental stages • Characterize kinetic parameters for enzymes in the shikimate/phenylpropanoid and MEP/terpenoid pathway for peppermint and sweet basil. • Measure levels of gene expression in glandular trichome secretory cells. • Measure protein levels for metabolic enzymes in glandular trichomes from different developmental stages. • Measure levels of phosphorylation for network enzymes. • Engineer proteins with altered properties. • Use transgenic/reengineered plants to evaluate the roles of specific protein modifications and of changes in their expression on carbon partitioning and metabolic flux in real world scenarios. Minimum Qualifications: Ph.D. in biochemistry, genomics, or modern plant sciences by date of hire. Excellent oral and written communication skills. Ability to work well as a member of an integrated research team. Preferred Qualifications: Documented expertise (as demonstrated by relevant publications) in two or more of the following four areas: 1. A strong background in use of next-generation sequencing technology (454/Roche sequencing, Solexa/Illumina sequencing, SOLiD sequencing). 2. A strong background in modern biochemical analysis of protein function, including expression of proteins in heterologous systems and purification of proteins from native and heterologous systems, analysis of protein activity using modern detection techniques, and enzyme activity assays. 3. Protein design and/or engineering modification to phosphorylation in proteins. 4. Metabolomics and/or proteomics, especially phosphoproteomics. To apply, please submit a cover letter, CV and the names and contact information for three references to: David R. Gang Institute of Biological Chemistry Clark 299 P.O. Box 646340 Pullman, WA 99164-6340 520-621-7154 gang@ag.arizona.edu Review of application materials will begin immediately. Washington State University is an EEO/AA Employer and Educator |
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