| 查看: 330 | 回复: 0 | ||
lize886622银虫 (正式写手)
|
[求助]
英译汉
|
|
The development of an assay for human neutrophit lipocalin (HNL) - to be used as a specific marker of neutrophil activity in vivo and vitro Abstract Human neutrophil lipocalin (HNL) is a newly discovered protein from human neutrophil secretory granules. A double-antibody radioimmunoassay (RIA) was developed for the measurement of HNL in various body fluids and its high specificity was confirmed by the absence of cross-reaction with other granulocyte granule proteins. The RIA measures HNL within the range of 4-256/~g/1. The intra- and interassay coefficients of variation were less than 6% and 10%, respectively. When HNL was added to serum samples full recovery was obtained. Sera and plasma from 100 apparently healthy individuals revealed a mean level of 78.40/~g/1 (range 37.95-190.87 tzg/1) in serum and a mean level of 50.65 pg/l (range 30.51-105.8 /zg/l) in EDTA-plasma. The distribution of HNL after gel filtration indicated that HNL exists mainly in two major forms, dimer and monomer. This, in addition to the excellent recovery, suggests that these major forms of HNL do not bind to compounds in serum or plasma that would interfere with the assay. The high specificity, sensitivity, reproducibility and accuracy of the present assay should facilitate the measurement of HNL in blood and other body fluids. |
回复此楼» 猜你喜欢
航天502所 高瑛珂博士 婚内征婚 欺骗女性开房
已经有30人回复
-
祈祷自己至少3A2B上会啊！
已经有5人回复
-
山东省优青 青B通知了？？？？
已经有3人回复
-
地球科学部D01口青年基金，最低几A几B几C才能有几率中呀。
已经有5人回复
-
26/27申博
已经有4人回复
