| 查看: 463 | 回复: 4 | |||
| 当前主题已经存档。 | |||
bioinfuture铁虫 (小有名气)
|
[交流]
求助一篇SCI文章，必有酬谢
|
||
|
Codon usage limitation in the expression of HIV-1 envelope glycoprotein.Haas J, Park EC, Seed B. Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA. BACKGROUND. The expression of both the env and gag gene products of human immunodeficiency virus type 1 (HIV-1) is known to be limited by cis elements in the viral RNA that impede egress from the nucleus and reduce the efficiency of translation. Identifying these elements has proven difficult, as they appear to be disseminated throughout the viral genome. RESULTS. Here, we report that selective codon usage appears to account for a substantial fraction of the inefficiency of viral protein synthesis, independent of any effect on improved nuclear export. The codon usage effect is not specific to transcripts of HIV-1 origin. Re-engineering the coding sequence of a model protein (Thy-1) with the most prevalent HIV-1 codons significantly impairs Thy-1 expression, whereas altering the coding sequence of the jellyfish green fluorescent protein gene to conform to the favored codons of highly expressed human proteins results in a substantial increase in expression efficiency. CONCLUSIONS. Codon-usage effects are a major impediment to the efficient expression of HIV-1 genes. Although mammalian genes do not show as profound a bias as do Escherichia coli genes, other proteins that are poorly expressed in mammalian cells can benefit from codon re-engineering. |
回复此楼» 猜你喜欢
哪位老哥知道今年的国自然具体哪一天放榜？
已经有6人回复
-
售SCI一区T0P文章，我:8O.55.1.O.5.4,科目齐全,可+急
已经有6人回复
-
售SCI文章，我:8O5.5.1.O.54,科目齐全,可+急
已经有7人回复
-
售SCI-T0P文章，我:8O.5.5.1.O.54,科目齐全,可+急
已经有5人回复
-
欢迎发来filecode的Mz6后的代码验证其规律
已经有50人回复
-
filecode
已经有11人回复
-
售SCI文章，我:8O.5.5.1O.54,科目全,可十急
已经有5人回复
-
售SCI一区T0P文章，我:8.O55.1.O.54,科目全,可十急
已经有5人回复
-
售SCI一区T0P文章，我:8.O55.1.O.54,科目全,可十急
已经有4人回复
-
售SCI文章，我:8O5.5.1.O.54,科目齐全,可+急
已经有4人回复
2楼2009-08-14 16:21:08
★ ★
生命核心(金币+2,VIP+0):感谢交流！ 8-14 19:15
生命核心(金币+2,VIP+0):感谢交流！ 8-14 19:15
|
哦 想起来了 不能直接 重新传下 http://www.brsbox.com/filebox/do ... 55ee3d574617685c89e [ Last edited by 随游 on 2009-8-18 at 17:37 ] |
3楼2009-08-14 16:22:34
cauzhangtao
木虫 (正式写手)
- 应助: 0 (幼儿园)
- 金币: 4297.5
- 散金: 13
- 红花: 3
- 帖子: 699
- 在线: 130小时
- 虫号: 547332
- 注册: 2008-04-17
- 性别: GG
- 专业: 分子与进化生态学
4楼2009-08-14 19:09:33
5楼2009-08-21 14:48:07
