| 查看: 816 | 回复: 2 | ||
| 【悬赏金币】回答本帖问题，作者Mr ironic将赠送您 5 个金币 | ||
| 当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖 | ||
Mr ironic新虫 (初入文坛)
|
[求助]
sod pod cat 联测的方法 已有1人参与
|
|
|
sod pod cat 联测的方法 发自小木虫Android客户端 |
回复此楼» 猜你喜欢
售SCI一区T0P文章，我:8.O.55.1.O.5.4,科目全,可+急
已经有3人回复
-
售SCI一区T0P文章，我:8O.55.1.O.5.4,科目齐全,可+急
已经有3人回复
-
售SCI文章，我:8O.5.5.1O.54,科目全,可十急
已经有3人回复
-
售SCI文章，我:8O5.5.1.O.54,科目齐全,可+急
已经有3人回复
-
售SCI一区文章，我:8O5.5.1.O5.4,科目全,可伽急
已经有3人回复
-
售SCI-T0P文章，我:8O.5.5.1.O.54,科目齐全,可+急
已经有3人回复
-
售SCI一区T0P文章，我:8.O55.1.O.54,科目全,可十急
已经有4人回复
-
售SCI一区T0P文章，我:8.O.55.1.O54,科目全,可伽急
已经有4人回复
-
售SCI一区T0P文章，我:8.O55.1.O.54,科目全,可十急
已经有4人回复
-
售SCI一区T0P文章，我:8.O55.1.O.54,科目全,可十急
已经有4人回复
singchenyao
金虫 (正式写手)
- 应助: 13 (小学生)
- 金币: 3469.7
- 散金: 82
- 红花: 5
- 帖子: 477
- 在线: 287.3小时
- 虫号: 1159599
- 注册: 2010-12-01
- 性别: GG
- 专业: 农学基础
3楼2020-10-12 15:58:27
singchenyao
金虫 (正式写手)
- 应助: 13 (小学生)
- 金币: 3469.7
- 散金: 82
- 红花: 5
- 帖子: 477
- 在线: 287.3小时
- 虫号: 1159599
- 注册: 2010-12-01
- 性别: GG
- 专业: 农学基础
【答案】应助回帖
|
我这里用传统的方法测定。我前段时间测的。 Superoxide dismutase (SOD) activity was analyzed using the nitroblue-tetrazolium (NBT) photoreduction method at 560 nm. One unit of SOD activity was defined as the amount of the enzyme that inhibited NBT reduction by 50% and was expressed as units g−1 fresh weight. For the catalase (CAT) activity assay, the reaction mixture contained 2 ml of 50 mM potassium phosphate buffer (pH 7.0), 1 ml of 15 mM H2O2 and 150 µl of the enzyme extract. The reaction was initiated with the addition of hydrogen peroxide, and the decrease in absorbance was measured at 240 nm for 2 min. CAT activity was expressed as the change in absorbance per min per fresh weight (ΔOD240 min-1 g-1 FW). For the determination of peroxidase (POD) activity, 50 μl of crude extract was added to 3 ml of substrate mixture containing 0.1 M potassium phosphate buffer (pH 6), 15 mM guaiacol and 7.5 mM of 30% H2O2. The formation of the oxidized tetraguaiacol polymer was monitored at 470 nm for 3 min. |
2楼2020-10-12 15:40:31
20