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关于NO测定和计算问题
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因为蔬菜组织本身含有亚硝酸盐，无法使用griess试剂法，采用血红蛋白分析法测定蔬菜组织NO，方法如下： NO accumulation was assayed and calculated by following the conversion of oxyhemoglobin (HbO2) to methemoglobin (MetHb) spectrophotometrically at 401 nm and 421 nm, using an extinction coefficient of 77 mm−1 cm−1 (A401 HbO2, A421 MetHb). the samples were homogenized in a mortar with 100 mM K-phosphate buffer (pH 7.0) and 0.6% (w:v) insoluble polyvinylpolypyrrolidone. The extract was clarified by adding powdered activated carbon and centrifuged at 11 000 × g for 10 min at 4 ◦C. The supernatant was filtered through a PTFE Millipore membrane (0.45 m) and immediately assayed for NO. Five minutes before oxyhemoglobin addition, samples were pretreated with catalase (100 U) and superoxide dismutase (100 U) to remove ROS. 问题来了，这个怎么计算结果的？ |
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