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| 实时荧光定量PCR（ real-time quantitative PCR）是在定性PCR技术基础上发展起来的核酸定量技术。该技术不仅实现了PCR从定性到定量的飞跃,而且与常规PCR相比,它具有特异性更强,PCR污染少,自动化程度高等特点,目前成为分子生物学和其它领域的重要技术工具，并广泛应用于遗传病、病原体和肿瘤等方面的检测和诊断。本文就实时荧光定量PCR技术的主要原理、定量方法及目前在研究领域中主要的应用进行了概述。 |
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实时荧光定量PCR（ real-time quantitative PCR）是在定性PCR技术基础上发展起来的核酸定量技术。该技术不仅实现了PCR从定性到定量的飞跃,而且与常规PCR相比,它具有特异性更强,PCR污染少,自动化程度高等特点,目前成为分子生物学和其它领域的重要技术工具，并广泛应用于遗传病、病原体和肿瘤等方面的检测和诊断。本文就实时荧光定量PCR技术的主要原理、定量方法及目前在研究领域中主要的应用进行了概述。 Real-time quantitative PCR is a method to quantify nucleic acid developed from qualitative PCR technique. This technology was successful for not only achievement from qualitative to quantitative, but also specification, less pollution, and excellent automation compared with conventional PCR, so that it has become crucial technical tool among molecular biology and other fields, as well as widely used as detection and diagnosis ways in genetic diseases, pathogens and tumor areas. The main principles, quantitative methods and recent applications in research areas of real-time quantitative PCR are summarized in the paper. |
2楼2009-07-06 21:17:28
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| Real-time quantitative PCR is a qualitative PCR technique developed on the basis of the nucleic acid quantification. This technology achieves not only a great leap from qualitative to quantitative, but also specifics to be more specific, less polluted, and highly automated compared to conventional PCR. Real-time quantitative PCR has become an important tool in molecular biology and other fields. It is widely used to perform detection and diagnosis in genetic diseases, pathogens and tumors. This paper reviews the main principles, quantitative methods and current applications of real-time fluorescence quantitative PCR. |
3楼2009-07-06 21:18:59
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小改一下： Real-time quantitative PCR is a qualitative PCR technique developed on the basis of the nucleic acid quantification. This technology achieves not only a great leap from qualitative to quantitative, but also the qualities to be more specific, less polluted, and highly automated compared to conventional PCR. Real-time quantitative PCR has become an important tool in molecular biology and other fields. It is widely used to perform detection and diagnosis in genetic diseases, pathogens and tumors. This paper reviews main principles, quantitative methods and current applications of real-time fluorescence quantitative PCR. |
4楼2009-07-06 21:38:03