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求助 英文摘要翻译！（20金币奉上）
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标题：Formulation of bovine respiratory syncytial virus fusion protein with CpG oligodeoxynucleotide, cationic host defense peptide and polyphosphazene enhances humoral and cellular responses and induces a protective type 1 immune response in mice. 摘要：Respiratory syncytial virus (RSV) is the leading cause of serious respiratory tract disease in children and calves; however, RSV vaccine development has been slow due to early observations that formalin-inactivated vaccines induced Th2-type immune responses and led to disease enhancement upon subsequent exposure. Hence, there is a need for novel adjuvants that will promote a protective Th1-type immune response against RSV. CpG oligodeoxynucleotides (ODNs), indolicidin, and polyphosphazene were examined for their ability to enhance antigen-specific immune responses and influence the Th-bias when co-formulated with a recombinant truncated bovine RSV (BRSV) fusion protein (DeltaF). Mice immunized with DeltaF co-formulated with CpG ODN, indolicidin, and polyphosphazene (DeltaF/CpG/indol/PP) developed higher levels of DeltaF-specific serum IgG, IgG1, and IgG2a antibodies when compared to DeltaF alone, and displayed an increase in the frequency of IFN-gamma-secreting cells and decreased IL-5 production by in vitro re-stimulated splenocytes, characteristic of a Th1 immune response. These results were observed in both C57BL/6 and Balb/c strains of mice. When evaluated in a BRSV challenge model, mice immunized with DeltaF/CpG/indol/PP developed significantly higher levels of BRSV-neutralizing serum antibodies than mice immunized with the DeltaF protein alone, and displayed significantly less pulmonary IL-4, IL-5, IL-13, and eotaxin and reduced eosinophilia after challenge. These results suggest that co-formulation of DeltaF with CpG ODN, host defense peptide and polyphosphazene may result in a safe and effective vaccine for the prevention of BRSV and may have implications for the development of novel human RSV vaccines. |
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2楼2009-07-04 22:44:56
我金币快没了！！！体谅下 助人为乐！！！ 
3楼2009-07-05 06:56:31
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braynt24(金币+20,VIP+0):谢谢！ 7-5 23:13
braynt24(金币+20,VIP+0):谢谢！ 7-5 23:13
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牛的呼吸道合胞病毒融合蛋白与Cpg寡脱氧核苷酸，阳离子性宿主防御肽，聚磷腈的共同使用能增强体液和细胞应答，并诱导小鼠的防御性Type 1 应答 呼吸道合胞病毒（RSV）是引起儿童和牛犊呼吸道疾病的主要原因；然而，RSV疫苗的开发一直很缓慢，因为早期研究发现福尔马林失活疫苗能诱导Th2型免疫应答，并随后发现其将导致疾病增强（或恶化）。因此，需要一种新的佐药来增强对RSV的Th1型免疫应答。对CpG寡脱氧核苷酸（ODNs），indolicidin，和聚磷腈增强抗原特异性免疫应答和当与一个重组的截短的牛的RSV融合蛋白配合使用时影响Th偏爱性的能力进行了试验。用DeltaF与CpG ODN, indolicidin, 聚磷腈配合使用来免疫的小鼠与单独使用DeltaF相比，产生了更高水平的DeltaF特异性免疫血清 IgG, IgG1,和 IgG2a抗体，并表现出伽马干扰素分泌细胞频率的增加，以及通过体外重新激活的脾细胞降低的IL-5产量，这些都是Th1型免疫应答的性质。这些结果在C57BL/6和 Balb/c种类的小鼠中都能观察到。当在一个BRSV攻击模型中评估时，免疫了DeltaF/CpG/indol的小鼠与仅免疫了DeltaF蛋白的小鼠相比，产生了更高水平的 BRSV中和血清抗体，并表现出更少的肺部IL-4, IL-5, IL-13和趋化因子以及攻击后减少的嗜酸粒细胞增多。这些结果表明，DeltaF与Cpg寡脱氧核苷酸，阳离子性宿主防御肽和聚磷腈的共同使用可能导致一种抑制BRSV的安全，有效的疫苗，并有可能对人类 RSV 新疫苗的开发有暗示的作用 对免疫方面不是很懂，呵呵，参考一下 [ Last edited by yanlili on 2009-7-5 at 11:44 ] |
4楼2009-07-05 11:42:01
