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Beginning Molecular Biology Laboratory Manual---------
CHAPTER 1: General Laboratory Methods

Safety Procedures
Preparation of Solutions
Disposal of Buffers and Chemicals
Equipment
bullet Micropipets
bullet Using a pH meter
bullet Autoclave operating procedures
bullet Operating instructions for spectrophotometer
Working with DNA
Sterile Technique

CHAPTER 2: Instructions for Notebook Keeping

CHAPTER 3: Computer User's Guide


UNIX commands
File Transfer
The World Wide Web
The Wisconsin Package - gcg.

CHAPTER 4: Molecular Biology Methods

M.1: Preparation of genomic DNA from bacteria
M.2: PCR amplification of DNA
M.3: Restriction enzyme digestion of DNA
M.4: Phenol/chloroform extraction of DNA
M.5: Ethanol precipitation of DNA
M.6: Agarose gel electrophoresis
M.7: Transformation of E. coli by electroporation
M.8: Wizard PCR preps DNA purification system
M.9: Alternate method for purifying DNA from agarose gels
M.10: Transfection of mammalian cells using Lipofectamine (LTI)
M.11: Southern blotting
M.12: RT-PCR Protocol
M.13: Preparation of sequencing gels
M.14: Isolation of RNA from mammalian cells using RNAZOL (Teltest)

CHAPTER 5: Tissue Culture Methods

Types of cells grown in culture
Work area and equipment
Preservation and storage
Maintenance
Safety considerations
Tissue culture methods
Determining cell counts


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500: The Psychiatric Dimensions of Sleep Medicine
499: Hematopathology: Morphology, Immunophenotype, Cytogenetics, and Molecular Approaches
498: Cummings Otolaryngology: Head and Neck Surgery . 4-Volume Set
497: Radiology Review Manual
496: Sleisenger & Fordtran's Gastrointestinal & Liver Disease
495: Principles and Practice of Mechanical Ventilation, 2nd Edition
494: The Goodman and Gilman Manual of Pharmacology and Therapeutics 2007
493: Review of Medical Physiology 22 ed.
492: Hepatology 2009
491: Nature Immunology
490: Encyclopedia of Infectious Diseases: Modern Methodologies
489: Tropical Infectious Diseases: Principles, Pathogens & Practice
488: Modern Epidemiology
487: Research Methodology in the Medical and Biological Sciences
486: Microarrays: Preparation, Microfluidics, Detection Methods, and Biological Applications
485: Proteomics in Practice: A Guide to Successful Experimental Design 2nd ed.
484: Food lipids: chemistry, nutrition, and biotechnology
483: Sexual Dysfunction: The Brain-body Connection
482: Atlas of Urodynamics
481: Electroanatomical Mapping: An Atlas for Clinicians
480: Ventricular Arrhythmias and Sudden Cardiac Death: Mechanism, Ablation, and Defibrillation
479: Brain Hypoxia and Ischemia
478: Essentials of Medical Genomics
477: Swanton's Cardiology
476: Harrison's Manual of Oncology
475: Therapeutic Strategies in Cardiovascular Risk
474: Disorders of the Human Adrenal Cortex
473: Vascular Diseases of the Liver
472: Prion Disease: A Bibliography and Dictionary for Physicians, Patients, and Genome Researchers
471: Johnson's Practical Electromyography
470: Evolution in Health and Disease 2nd ed.
469: Heliobacter Pylori
468: Forensic Human Identification: An Introduction
467: Forensic Medicine
466: Forensic Science
465: Mitochondria
464: PCR (The Basics)
463: The Cancer Degradome
462: The Septins
461: Molecular Imaging in Oncology
460: Antimicrobial Resistance: Problem Pathogens and Clinical Countermeasures
459: Peptide Characterization and Application Protocols (Methods in Molecular Biology)
458: Tumor-Induced Immune Suppression Mechanisms and Therapeutic Reversal
457: Hormonal Balance: Understanding Hormones, Weight, and Your Metabolism
456: The Effects of Estrogen on Brain Function
455: Williams Textbook of Endocrinology
454: Handbook of Neurochemistry and Molecular Neurobiology
453: How the Immune System Recognizes Self and Nonself Immunoreceptors and Their Signaling
452: Proteomics in Nephrology - Towards Clinical Applications
451: Molecular Aspects of Innate and Adaptive Immunity
450: Dermatologic Immunity
449: Protein Purification Protocols (Methods in Molecular Biology)
448: B Cell Protocols (Methods in Molecular Biology)
447: Medicinal Protein Engineering
446: Aromatase Inhibitors (Milestones in Drug Therapy)
445: Laboratory Guide to the Methods in Biochemical Genetics
444: Progestins and the Mammary Gland: From Basic Science to Clinical Applications
443: Diagnosis and Management of Pituitary Disorders
442: Cardiovascular Hormone Systems: From Molecular Mechanisms to Novel Therapeutics
441: Van De Graaff Human Anatomy
440: Clinical Anatomy: Applied Anatomy for Students and Junior Doctors
439: Bioimpedance and Bioelectricity Basics
438: Patch-Clamp Methods and Protocols (Methods in Molecular Biology)
437: Respiratory Physiology: The Essentials Eighth Edition
436: Current Medical Diagnosis and Treatment 2009
435: Manual of Surgery, Volume 1: General Surgery
434: Dictionary of Parasitology
433: Clinical Neuroanatomy Made Ridiculously Simple 3rd ed,
432: Encyclopedia of Stress
431: Herbal Medicines, 3rd Edition
430: Gale Encyclopedia of Genetic Disorders 2nd ed.
429: Fundamentals of Cancer Prevention
428: Research Methods in Clinical Psychology: An Introduction for Students and Practitioners
427: Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems
426: Particle Toxicology
425: Visual Diagnosis in Emergency and Critical Care Medicine
424: Regional Nerve Blocks And Infiltration Therapy
423: Emergency Management of Infectious Diseases
422: Viruses and Human Disease
421: Genetics of Asthma and Chronic Obstructive Pulmonary Disease
420: Mayo Clinic Cardiology: Board Review Questions and Answers
419: Respiratory Notes Respiratory Therapist's Pocket Guide
418: Type 2 Diabetes: Principles and Practice, Second Edition
417: Heparin-Induced Thrombocytopenia, 4th Edition
416: Handbook of Chronic Total Occlusions
415: Breast Reconstruction
414: Therapist's Guide to Evidence-Based Relapse Prevention
413: Inhalants
412: Complications in Surgery and Trauma
411: Treatment Guidelines for Medicine and Primary Care
410: Harrison's Principles of Internal Medicine, 17th Edition
409: Braunwald's Heart Disease: A Textbook of Cardiovascular Medicine, 8th Edition
408: Nelson Textbook of Pediatrics, 18th e-edition
407: The Care of Wounds: A Guide for Nurses
406: Master Techniques in Orthopaedic Surgery: Soft Tissue Surgery
405: Desk Reference of Clinical Pharmacology
404: Atrial Fibrillation: From Bench to Bedside
403: Osteoporosis: Methods and Protocols
402: Grant's Atlas of Anatomy
401: Encyclopedia of Cancer
400: Clinical Trials in Rheumatoid Arthritis and Osteoarthritis
399: Current Essentials of Critical Care
398: Avoiding Common ICU Errors
397: Gastrointestinal Nursing
396: Drugs & Drugs: A Practical Guide To The Safe Use of Common Drugs in Adults 2nd ed.
395: Calculation Skills
394: Clinical Drug Therapy: Rationales for Nursing Practice
393: Lippincott Manual of Nursing Practice
392: Handbook of Preventive Interventions for Adults
391: Review of Medical Physiology
390: Gross Anatomy
389: Drug Hypersensitivity
388: Complementary Medicine For Dummies
387: Clinician's Pocket Drug Reference 2008
386: Environmental Factors in Skin Disease
385: Textbook of Cardiovascular Medicine 3rd ed.
384: Cardiovascular Medicine
383: Pharmaceutical Manufacturing Encyclopedia, 3rd Edition
382: Robbins Basic Pathology
381: Davidson's Principles and Practice of Medicine: with STUDENT CONSULT Access 20th ed.
380: Surgical Wound Healing and Management
379: Cultural Competence in Health Care
378: Navigation and MIS in Orthopedic Surgery
377: Medical Secrets, 4th Edition
376: Dermatology
375: Ophthalmic Drug Facts 2007
374: Current Medical Diagnosis & Treatment 2008
373: Pediatric Critical Care Review
372: Osteopetrosis - A Medical Dictionary, Bibliography and Annotated Research Guide to Web References
371: Emergency First Aid On Board
370: Dictionary of Medical Acronyms & Abbreviations 5th Edition
369: Advanced Manufacturing Technology for Medical Applications: Reverse Engineering, Software Conversion
368: Colorectal Cancer
367: Modern Hematology: Biology and Clinical Management 2nd Ed.
366: Atlas of Emergency Medicine
365: Rang & Dale's Pharmacology
364: Herbal Products: Toxicology and Clinical Pharmacology
363: Heart Failure: A Combined Medical and Surgical Approach
362: Developing Anaesthesia Textbook 2007
361: Making and Using Antibodies: A Practical Handbook
360: Handbook of Sleep Medicine, 2006
359: Differential Diagnosis in Primary Care 4th ed.
358: The 5-Minute Clinical Consult, 2008
357: E-Health Care Information Systems : An Introduction for Students and Professionals
356: Where There Is No Doctor
355: The Gale Encyclopedia of Medicine 3rd Edition
354: Polyomaviruses and Human Diseases
353: Resveratrol in Health and Disease
352: Microarray Technology and Cancer Gene Profiling
351: Robotic Radiosurgery.Treating Tumors that Move with Respiration
350: Glaucoma Medical Therapy: Principles and Management
349: Novel Anticancer Agents: Strategies for Discovery and Clinical Testing
348: Visualization in Medicine: Theory, Algorithms, and Applications
347: Complementary and Alternative Therapies for Epilepsy
346: Diagnostic Atlas of Common Eyelid Diseases
345: Advances in Functional and Reparative Neurosurgery
344: The Art and Science of Cardiac Physical Examination
343: Cardiovascular Biomarkers: Pathophysiology and Disease Management
342: Cardiovascular Molecular Imaging
341: The Handbook of Contraception: A Guide for Practical Management
340: Hypertension and Hormone Mechanisms
339: Diagnostic Criteria in Neurology
338: Nanotoxicology: Characterization, Dosing and Health Effects
337: Radiology
336: Mind from Body: Experience from Neural Structure
335: Cardiac Gene Expression: Methods and Protocols
334: PRINS and In Situ PCR Protocols
333: Professional Guide to Signs and Symptoms 5th Edition
332: Botulinum Toxin in Aesthetic Medicine
331: Principles and Practice of Surgery for the Colon, Rectum, and Anus Third Edition
330: Oral Surgery
329: Taylor's 10-Minute Diagnosis Manual: Symptoms and Signs in the Time-Limited Encounter
328: Handbook of Critical Care Drug Therapy
327: Hughes' Outline of Modern Psychiatry
326: Rapid Surgery
325: The Internal Medicine Casebook: Real Patients, Real Answers
324: Oxford Textbook of Philosophy and Psychiatry
323: Northwestern Handbook of Surgical Procedures
322: Current Diagnosis & Treatment of Pain
321: Epilepsy: Global Issues For The Practicing Neurologist : World Federation of Neurology, Seminars
320: Color Atlas of Histopathology of the Cervix Uteri
319: Human Embryonic Stem Cell Protocols
318: Comprehensive Gynecology
317: Ultrasonography in Vascular Diagnosis: A Therapy-Oriented Textbook and Atlas
316: Exercise-Induced Acute Renal Failure: Acute Renal Failure with Severe Loin Pain and Patchy Renal
315: Varney's Midwifery, Fourth Edition
314: The Thermodynamic Machinery of Life
313: Murray and Nadel's Textbook of Respiratory Medicine
312: Skin Aging
311: The Massachusetts General Hospital Handbook 3rd ed.
310: The Emerging Physics of Consciousness
309: Us Army Medical Course - Dental Anatomy And Physiology (2006 Edition)
308: The Clinician’s Guide to Gastrointestinal Oncology
307: Ferri’s Clinical Advisor 2007: Instant Diagnosis and Treatment, Textbook, Website & PocketConsult
306: Greenspan's Basic & Clinical Endocrinology
305: Handbook of Medicinal Herbs, Second Edition
304: Be Your Own Doctor with Foot Reflexology
303: An Illustrated Chinese Materia Medica
302: Clinician's Pocket Reference
301: The Skeleton
300: USP NF 2007 (United States Pharmacopeia/National Formulary)
299: Recent Developments in Alcoholism : Alcohol Problems in Adolescents and Young Adults
298: An Issue of Critical Care Nursing Clinics
297: Oxford Handbook of Critical Care 2nd ed.
296: Communicable Disease Control in Emergencies: A Field Manual
295: A Pocket Book of the European Resuscitation Council Guidelines for Resuscitation 2005
294: Pharmacotherapy, an Issue of Critical Care Clinics
293: Nanotechnology in Cancer Therapeutics
292: Brain Tumor Pathology
291: Gynecologic Cancer
290: Head and Neck Cancer Imaging
289: AJCC Cancer Staging Atlas
288: Bone Age Atlas
287: Environmental UV Radiation: Impact on Ecosystems and Human Health and Predictive Models
286: Gastroesophageal Reflux Disease
285: Just the Facts in Pediatrics
284: Atlas of Cardiometabolic Risk
283: Electronic Atlas of Hematology
282: Imaging of the Hip & Bony Pelvis
281: Bone and Cartilage Engineering
280: Operative Gynecology 9th Ed.
279: Handbook of Pharmaceutical Granulation Technology
278: Revision Total Knee Arthroplasty
277: Critical Care Focus 8: Blood and Blood Transfusion
276: Data Monitoring in Clinical Trials
275: Total Ankle Arthroplasty
274: Focal Liver Lesions: Detection, Characterization, Ablation
273: An Atlas of Headache
272: Growing Bone
271: Contrast Media Safety Issues and ESUR Guidelines
270: The Essential Handbook of Treatment and Prevention of Alcohol Problems
269: Diabetes Mellitus: Methods and Protocols (Methods in Molecular Medicine, 83)
268: Emergency Care for Children: Growing Pains
267: Kaplan & Sadock's Comprehensive Textbook of Psychiatry, 7th Edition
266: Field Guide to Bedside Diagnosis
265: ABC of Eyes
264: The Biology of Dendritic Cells and HIV Infection
263: Football Traumatology: Current Concepts: from Prevention to Treatment
262: Urology Secrets
261: Manual of Internal Medicine
260: Principles of Critical Care, Third Edition
259: USMLE Mnemonics and Topics
258: Neurology Study Guide: Oral Board Examination Review
257: Usmle Pretest - Behavioral Sciences
256: Pathology: PreTest Self-Assessment and Review
255: Pretest Physiology
254: Kaplan Notes 2002 Pharmacology
253: USMLE STEP 1 Kaplan Notes
252: ABC of Liver, Pancreas and Gall Bladder
251: Sabiston & Spencer Surgery of the Chest: 2-Volume Set
250: The Doctor’s Guide to Gastrointestinal Health: Preventing and Treating Acid Reflux, Ulcers
249: Fungal Infection: Diagnosis and Management
248: Dynamics Of The Vascular System
247: The Gale Encyclopedia of Neurological Disorders
246: AZ of Emergency Radiology
245: Essentials of Thoracic Imaging, An Issue of Radiologic Clinics
244: Radiology for Anaesthesia and Intensive Care
243: The WHO Manual on Diagnostic Imaging
242: Abnormalities In Puberty Scientific And Clinical Advances
241: Pharmaceutical Experimental Design and Interpretation, 2nd Edition
240: Pharmaceutical Engineering Change Control Second Edition
239: Pharmaceutical Biotechnology, Second Edition
238: Handbook of Near-Infrared Analysis, Second Edition
237: PARP as a Therapeutic Target
236: Parenteral Quality Control Sterility Pyrogen Particulate and Package Integrity Testing 3rd ed.
235: Impaction Bone Grafting in Revision Arthroplasty
234: The Encyclopedia of Endocrine Diseases and Disorders
233: Review of Surgery: Basic Science and Clinical Topics for ABSITE
232: Free Medical Information
231: Models Of Viral Hepatitis (Monographs in Virology)
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