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【讨论】请教大家一个关于荧光的问题，谢谢
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荧光共振能量转移（FRET）给体与供体之间的能量传递方式是非辐射能量传递。请教各位大虾，非辐射能量传递是什么含义？老板说是供体发出荧光给 给体，使得给体被激发，这一说法我很怀疑 |
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2楼2009-06-20 16:28:48
3楼2009-06-20 21:37:06
jhn183
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| FRET occurs between a donor (D) molecule in the excited state and an acceptor (A) molecule in the ground state. The donor molecules typically emit at shorter wavelengths that overlap with the absorption spectrum of the acceptor. Energy transfer occurs without the appearance of a photon and is the result of longrange dipole–dipole interactions between the donor and acceptor. The term resonance energy transfer (RET) is preferred because the process does not involve the appearance of a photon. The rate of energy transfer depends upon the extent of spectral overlap of the emission spectrum of the donor with the absorption spectrum of the acceptor, the quantum yield of the donor, the relative orientation of the donor and acceptor transition dipoles, and the distance between the donor and acceptor molecules. The distance dependence of RET allows measurement of the distances between donors and acceptors. |
4楼2009-06-20 23:53:22
5楼2009-06-21 11:19:06