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:pÓÐûÓÐÈË×ö¹ýÀàËÆµÄ°¡£¬ÎÒ¿´Ò½Ñ§ÉÏÒ²ÓÐÀàËÆµÄÈÜÒºÅä±È£¬ÇóÖúÄÚÈݸÅÀ¨ÊÇ3.6%µÄaÓë0.2%µÄb»ìºÏ£¬ÆäÖк¬ÓÐ10%µÄcºË0.1%µÄd£¬²¢²¹³äeºÍf£¨ef¾ùÊǸøÈÜҺŨ¶È£©£¬ÓÐûÓдóÉñ×ö¹ý£¬¸ø¸öÈÜҺȡÁ¿£¬»òÕßÒ»¸öÈ¡Á¿·¶Î§£¬ÎÒ×Ô¼º³¢ÊÔÒ²¿ÉÒÔ:p ÔÎÄÈçÏ£º for light microscopy and immunofluorescence microscopy,samples were fixed overnight at room temperature, in a mixture of 3.6% paraformaldehyde and 0.2% glutaraldehyde that contained 10% dimethylsulfoxide and 0.1% nonidet p-40 in 50 mm piperazine-1,4-bis(2-ethanesulfonic acid) buffer (ph 7.0), supplemented with 5 mm ethylene glycol-bis(¦Â-aminoethyl ether)-n,n,n' ,n' - tetraacetic acid and 5mm mgso 4 .radial sections of approximately 40¦Ìm thickness were cut on a freezing stage of a sliding microtome (ma-101; komatsu electronics, tokyo, japan) and washed with phosphate-buffered saline (pbs; 137 mm nacl, 2.7 mm kcl, 1.5 mm kh 2 po 4 , 8.0 mm na 2 hpo 4 ,adjusted to ph 7.3). Óùȸè·ÒëÈçÏ£º ¶ÔÓÚ¹âѧÏÔ΢¾µºÍÃâÒßÓ«¹âÏÔ΢¾µ£¬½«ÑùÆ·ÔÚÊÒÎÂϹ̶¨¹ýÒ¹£¬ÔÚ3.6£¥¶à¾Û¼×È©ºÍ0.2£¥Îì¶þÈ©µÄ»ìºÏÎïÖУ¬Æäº¬ÓÐ10£¥¶þ¼×»ùÑÇí¿ºÍ0.1£¥nonidet p-40µÄ50mmßßàº-1,4-¶þ£¨2- ÒÒ»ÇËᣨethanesulfonic acid£©»º³åÒº£¨ph7.0£©£¬²¹³ä5mmÒÒ¶þ´¼ - Ë«£¨¦Â-°±»ùÒÒ»ùÃÑ£©-n£¬n£¬n'£¬n' - ËÄÒÒËáºÍ5mm mgso 4¡£ÇÐÏÂÔ¼40¦ÌmºñµÄ·ÅÉäÐÔÇÐÆ¬ »¬¶¯ÇÐÆ¬»úµÄÀä¶³½×¶Î£¬²¢ÓÃÁ×ËáÑλº³åÑÎË®£¨pbs; 137mm nacl£¬2.7mm kcl£¬1.5mm kh 2 po 4,8.0mm na 2 hpo 4£¬µ÷½ÚÖÁph 7.3£©Ï´µÓ¡£ [ Last edited by ÁúÁèÒôa on 2019-10-1 at 21:40 ] |
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