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for light microscopy and immunofluorescence microscopy,samples were fixed overnight at room temperature, in a mixture of 3.6% paraformaldehyde and 0.2% glutaraldehyde that contained 10% dimethylsulfoxide and 0.1% nonidet p-40 in 50 mm piperazine-1,4-bis(2-ethanesulfonic acid) buffer (ph 7.0), supplemented with 5 mm ethylene glycol-bis(¦Â-aminoethyl ether)-n,n,n' ,n'  - tetraacetic acid and 5mm mgso 4 .radial sections of approximately 40¦Ìm thickness were cut on a freezing stage of a sliding microtome (ma-101; komatsu electronics, tokyo, japan) and washed with phosphate-buffered saline (pbs; 137 mm nacl, 2.7 mm kcl, 1.5 mm kh 2 po 4 , 8.0 mm na 2 hpo 4 ,adjusted to ph 7.3).
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[ Last edited by ÁúÁèÒôa on 2019-10-1 at 21:40 ]
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