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[交流] 【分享】一本值得收藏pcr英文书籍【搜索无重复】

本着资源共享的原则，现拿出来大家一起分享，希望大家捧个场，你的支持就是我不竭的动力。。。。。。。。
PCR Cloning protocols
纳米盘;
http://www.namipan.com/d/5b51e16 ... 7f41c97613180841e00
http://www.namipan.com/d/0188c9a ... 786a3d9732c06ee0200

内容简介
PART I. PERFORMING AND OPTIMIZING PCR
1 Polymerase Chain Reaction: Basic Principles and Routine Practice
Lori A. Kolmodin and David E. Birch .................................................. 3
2 Computer Programs for PCR Primer Design and Analysis
Bing-Yuan Chen, Harry W. Janes, and Steve Chen ........................ 19
3 Single-Step PCR Optimization
Using Touchdown and Stepdown PCR Programming
Kenneth H. Roux .................................................................................. 31
4 XL PCR Amplification of Long Targets from Genomic DNA
Lori A. Kolmodin .................................................................................. 37
5 Coupled One-Step Reverse Transcription and Polymerase Chain
Reaction Procedure for Cloning Large cDNA Fragments
Jyrki T. Aatsinki ................................................................................... 53
6 Long Distance Reverse-Transcription PCR
Volker Thiel, Jens Herold, and Stuart G. Siddell ............................. 59
7 Increasing PCR Sensitivity for Amplification
from Paraffin-Embedded Tissues
Abebe Akalu and Juergen K. V. Reichardt ....................................... 67
8 GC-Rich Template Amplification by Inverse PCR:
DNA Polymerase and Solvent Effects
Alain Moreau, Da Shen Wang, Steve Forget, Colette Duez,
and Jean Dusart............................................................................... 75
9 PCR Procedure for the Isolation of Trinucleotide Repeats
Teruaki Tozaki ...................................................................................... 81
10 Methylation-Specific PCR
Haruhiko Ohashi .................................................................................. 91
23 A Fast Polymerase Chain Reaction-Mediated Strategy for Introducing
Repeat Expansions into CAG-Repeat Containing Genes
Franco Laccone ................................................................................. 217
24 PCR Screening in Signature-Tagged Mutagenesis of Essential Genes
Dario E. Lehoux and Roger C. Levesque ....................................... 225
25 Staggered Extension Process (StEP) In Vitro Recombination
Anna Marie Aguinaldo and Frances Arnold ................................... 235
26 Random Mutagenesis by Whole-Plasmid PCR Amplification
Donghak Kim and F. Peter Guengerich .......................................... 241
PART IV. CLONING UNKNOWN NEIGHBORING DNA
27 PCR-Based Strategies to Clone Unknown DNA Regions
from Known Foreign Integrants: An Overview
Eric Ka-Wai Hui, Po-Ching Wang, and Szecheng J. Lo ................ 249
28 Long Distance Vectorette PCR (LDV PCR)
James A. L. Fenton, Guy Pratt, and Gareth J. Morgan ................. 275
29 Nonspecific, Nested Suppression PCR Method
for Isolation of Unknown Flanking DNA (“Cold-Start Method”)
Michael Lardelli .................................................................................. 285
30 Inverse PCR: cDNA Cloning
Sheng-He Huang ................................................................................ 293
31 Inverse PCR: Genomic DNA Cloning
Ambrose Y. Jong, Anna T’ang, De-Pei Liu,
and Sheng-He Huang .................................................................... 301
32 Gene Cloning and Expression Profiling by Rapid Amplification
of Gene Inserts with Universal Vector Primers
Sheng-He Huang, Hua-Yang Wu, and Ambrose Y. Jong .............. 309
33 The Isolation of DNA Sequences Flanking Tn5 Transposon Insertions
by Inverse PCR
Vincent J. J. Martin and William W. Mohn ...................................... 315
34 Rapid Amplification of Genomic DNA Sequences Tagged
by Insertional Mutagenesis
Martina Celerin and Kristin T. Chun ................................................ 325
35 Isolation of Large Terminal Sequences of BAC Inserts Based
on Double-Restriction-Enzyme Digestion Followed
by Anchored PCR
Zhong-Nan Yang and T. Erik Mirkov ............................................... 337
36 A “Step Down” PCR-Based Technique for Walking
Into and the Subsequent Direct Sequence Analysis
of Flanking Genomic DNA
Ziguo Zhang and Sarah Jane Gurr .................................................. 343
PART V. LIBRARY CONSTRUCTION AND SCREENING
37 Use of PCR in Library Screening: An Overview
Jinbao Zhu .......................................................................................... 353
38 Cloning of Homologous Genes by Gene-Capture PCR
Renato Mastrangeli and Silvia Donini ............................................. 359
39 Rapid and Nonradioactive Screening of Recombinant Libraries by PCR
Michael W. King ................................................................................. 377
40 Rapid cDNA Cloning by PCR Screening (RC-PCR)
Toru Takumi ....................................................................................... 385
41 Generation and PCR Screening of Bacteriophage λ Sublibraries
Enriched for Rare Clones (the “Sublibrary Method”)
Michael Lardelli .................................................................................. 391
42 PCR-Based Screening for Bacterial Artificial Chromosome Libraries
Yuji Yasukochi ................................................................................... 401
43 A 384-Well Microtiter-Plate-Based Template Preparation
and Sequencing Method
Lei He and Kai Wang ......................................................................... 411
44 A Microtiter-Plate-Based High Throughput PCR Product
Purification Method
Ryan Smith and Kai Wang ................................................................ 417
值的收藏的一本书

[ Last edited by c3024034 on 2009-6-11 at 21:07 ]

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