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Scanometric DNA array detection with nanoparticle probes SCIENCE 卷: 289 期: 5485 页: 1757-1760 出版年: SEP 8 2000 Taton TA, Mirkin CA, Letsinger RL |
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2楼2009-05-31 14:06:00
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Bibliographic Information Scanometric DNA array detection with nanoparticle probes. Taton T A; Mirkin C A; Letsinger R L Department of Chemistry, Center for Nanofabrication and Molecular Self-Assembly, Northwestern University, Evanston, IL 60208, USA Science (New York, N.Y.) (2000), 289(5485), 1757-60. Journal code: 0404511. ISSN:0036-8075. United States. (COMPARATIVE STUDY); Journal; Article; (JOURNAL ARTICLE); (RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.); (RESEARCH SUPPORT, U.S. GOV'T, P.H.S.) written in English. PubMed ID 10976070 AN 2000440734 MEDLINE Abstract A method for analyzing combinatorial DNA arrays using oligonucleotide-modified gold nanoparticle probes and a conventional flatbed scanner is described here. Labeling oligonucleotide targets with nanoparticle rather than fluorophore probes substantially alters the melting profiles of the targets from an array substrate. This difference permits the discrimination of an oligonucleotide sequence from targets with single nucleotide mismatches with a selectivity that is over three times that observed for fluorophore-labeled targets. In addition, when coupled with a signal amplification method based on nanoparticle-promoted reduction of silver(I), the sensitivity of this scanometric array detection system exceeds that of the analogous fluorophore system by two orders of magnitude. Controlled Terms Base Pair Mismatch Base Pairing Carbocyanines Fluorescent Dyes Gold Microscopy, Confocal Microscopy, Fluorescence Nucleic Acid Hybridization Oligonucleotide Array Sequence Analysis: IS, instrumentation *Oligonucleotide Array Sequence Analysis: MT, methods *Oligonucleotide Probes Sensitivity and Specificity Temperature Registry Numbers 7440-57-5 (Gold) Chemical Names 0 (Carbocyanines) 0 (Fluorescent Dyes) 0 (Oligonucleotide Probes) 0 (cyanine dye 3) |
4楼2009-05-31 15:40:11
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Bibliographic Information Scanometric DNA array detection with nanoparticle probes. Taton T A; Mirkin C A; Letsinger R L Department of Chemistry, Center for Nanofabrication and Molecular Self-Assembly, Northwestern University, Evanston, IL 60208, USA Science (New York, N.Y.) (2000), 289(5485), 1757-60. Journal code: 0404511. ISSN:0036-8075. United States. (COMPARATIVE STUDY); Journal; Article; (JOURNAL ARTICLE); (RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.); (RESEARCH SUPPORT, U.S. GOV'T, P.H.S.) written in English. PubMed ID 10976070 AN 2000440734 MEDLINE Abstract A method for analyzing combinatorial DNA arrays using oligonucleotide-modified gold nanoparticle probes and a conventional flatbed scanner is described here. Labeling oligonucleotide targets with nanoparticle rather than fluorophore probes substantially alters the melting profiles of the targets from an array substrate. This difference permits the discrimination of an oligonucleotide sequence from targets with single nucleotide mismatches with a selectivity that is over three times that observed for fluorophore-labeled targets. In addition, when coupled with a signal amplification method based on nanoparticle-promoted reduction of silver(I), the sensitivity of this scanometric array detection system exceeds that of the analogous fluorophore system by two orders of magnitude. Controlled Terms Base Pair Mismatch Base Pairing Carbocyanines Fluorescent Dyes Gold Microscopy, Confocal Microscopy, Fluorescence Nucleic Acid Hybridization Oligonucleotide Array Sequence Analysis: IS, instrumentation *Oligonucleotide Array Sequence Analysis: MT, methods *Oligonucleotide Probes Sensitivity and Specificity Temperature Registry Numbers 7440-57-5 (Gold) Chemical Names 0 (Carbocyanines) 0 (Fluorescent Dyes) 0 (Oligonucleotide Probes) 0 (cyanine dye 3) |
5楼2009-05-31 15:42:32