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[求助]
跪求pMG36e载体上红霉素抗性基因序列，往各路大神帮帮忙已有1人参与
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| 如题，因为要用红霉素基因，但是不知道其序列。跪求pMG36e载体上红霉素抗性基因序列，倾其所有金币，往各路大神帮帮忙。 |
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snoopyzxx
木虫 (著名写手)
- MolEPI: 4
- 应助: 49 (小学生)
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- 专业: 全球变化生态学
【答案】应助回帖
感谢参与，应助指数 +1
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1463——2197就是你要的红霉素抗性基因。 pMG36e Plasmid Information Promoter: P32 Replicator: pWV01 ori Terminator: T Plasmid classification: Lactococcus lactis constitutively expressed plasmid Plasmid size: 3610bp Resistance: Erythromycin Tag: Erythromycin Clone strain: DH5 alpha Culture conditions: 37 DEG C, aerobic, LB Host: Lactococcus lactis Induction mode: no need to induce Primers for 5'sequencing: Based on sequence design Primers for 3'sequencing: Based on sequence design Expression: Bacterial Use:Lactic Acid Bacteria Plasmids pMG36e Plasmid Sequence LOCUS Exported 3610 bp ds-DNA circular SYN 14-JUL-2016 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pMG36e SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3610) AUTHORS . TITLE Direct Submission JOURNAL Exported Thursday, July 14, 2016 from SnapGene Viewer 3.1.4 FEATURES Location/Qualifiers source 1..3610 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 227..925 /codon_start=1 /note="repA" /translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMDE KKDKDTWNSSDVIRNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEILD YIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDRYITLDESQKRELKNLLLDIV DDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRASYAK VLDAETGEIK" CDS complement(1463..2197) /codon_start=1 /note="Emr" /translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHFT LELVKRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIYGNIPY NISTDIIRKIVFDSIANEIYLIVEYGFAKRLLNTKRSLALLLMAEVDISILSMVPREYF HPKPKVNSSLIRLSRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKHAGID DLNNISFEQFLSLFNSYKLFNK" misc_feature 2474..2652 /note="P32" CDS 2653..2892 /codon_start=1 /note="ORF" /translation="MAIVSAEKFVIRARPGIDPLESTCRHASLQSLKTKVAAAVEAAKT VGKGDGTTGTSDKGGGQGTPALRYLELRFKVKWY" CDS complement(3215..3226) /codon_start=1 /product="Factor Xa recognition and cleavage site" /note="Factor Xa site" /translation="IEGR" ORIGIN 1 ggtgatttca gaatcgaaaa aaagagttat gatttctctg acaaaagagc aagataaaaa 61 attaacagat atggcgaaac aaaaaggttt ttcaaaatct gcggttgcgg cgttagctat 121 agaagaatat gcaagaaagg aatcagaaca aaaaaaataa gcgaaagctc gcgtttttag 181 aaggatacga gttttcgcta cttgtttttg ataaggtaat tatatcatgg ctattaaaaa 241 tactaaagct agaaattttg gatttttatt atatcctgac tcaattccta atgattggaa 301 agaaaaatta gagagtttgg gcgtatctat ggctgtcagt cctttacacg atatggacga 361 aaaaaaagat aaagatacat ggaatagtag tgatgttata cgaaatggaa agcactataa 421 aaaaccacac tatcacgtta tatatattgc acgaaatcct gtaacaatag aaagcgttag 481 gaacaagatt aagcgaaaat tggggaatag ttcagttgct catgttgaga tacttgatta 541 tatcaaaggt tcatatgaat atttgactca tgaatcaaag gacgctattg ctaagaataa 601 acatatatac gacaaaaaag atattttgaa cattaatgat tttgatattg accgctatat 661 aacacttgat gaaagccaaa aaagagaatt gaagaattta cttttagata tagtggatga 721 ctataatttg gtaaatacaa aagatttaat ggcttttatt cgccttaggg gagcggagtt 781 tggaatttta aatacgaatg atgtaaaaga tattgtttca acaaactcta gcgcctttag 841 attatggttt gagggcaatt atcagtgtgg atatagagca agttatgcaa aggttcttga 901 tgctgaaacg ggggaaataa aatgacaaac aaagaaaaag agttatttgc tgaaaatgag 961 gaattaaaaa aagaaattaa ggacttaaaa gagcgtattg aaagatacag agaaatggaa 1021 gttgaattaa gtacaacaat agatttattg agaggaggga ttattgaata aataaaagcc 1081 ccctgacgaa agtcgaaggg ggtttttatt ttggtttgat gttgcgatta atagcaatac 1141 aattgcaata aacaaaatga tcgacctcgg gacccctatc tagcgaactt ttagaaaaga 1201 tataaaacat cagagtatgg acagttgcgg atgtacttca gaaaagatta gatgtctaaa 1261 aagctagctt tttagacatc taaatctagg tactaaaaca attcatccag taaaatataa 1321 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac 1381 tgttcttccc cgatcgaccc gattcacaaa aaataggcac acgaaaaaca agttaaggga 1441 tgcagtttat gcatccctta acttacttat taaataattt atagctattg aaaagagata 1501 agaattgttc aaagctaata ttgtttaaat cgtcaattcc tgcatgtttt aaggaattgt 1561 taaattgatt ttttgtaaat attttcttgt attctttgtt aacccatttc ataacgaaat 1621 aattatactt ttgtttatct ttgtgtgata ttcttgattt ttttctactt aatctgataa 1681 gtgagctatt cactttaggt ttaggatgaa aatattctct tggaaccata cttaatatag 1741 aaatatcaac ttctgccatt aaaagtaatg ccaatgagcg ttttgtattt aataatcttt 1801 tagcaaaccc gtattccacg attaaataaa tctcattagc tatactatca aaaacaattt 1861 tgcgtattat atccgtactt atgttataag gtatattacc atatatttta taggattggt 1921 ttttaggaaa tttaaactgc aatatatcct tgtttaaaac ttggaaatta tcgtgatcaa 1981 caagtttatt ttctgtagtt ttgcataatt tatggtctat ttcaatggca gttacgaaat 2041 tacacctctt tactaattca agggtaaaat ggccttttcc tgagccgatt tcaaagatat 2101 tatcatgttc atttaatctt atatttgtca ttattttatc tatattatgt tttgaagtaa 2161 taaagttttg actgtgtttt atatttttct cgttcattat aaccctcttt aatttggtta 2221 tatgaatttt gcttattaac gattcattat aaccacttat tttttgtttg gttgataatg 2281 aactgtgctg attacaaaaa tactaaaaat gcccatattt tttcctcctt ataaaattag 2341 tataattata gcacggtcga tcttctatat aaaagatata ttatcttatc agtattgtca 2401 atatattcaa ggcaatctgc ctcctcatcc tcttcatcct cttcgtcttg gtagcttttt 2461 aaatatgggt cgatcgaatt cggtcctcgg gatatgataa gattaatagt tttagctatt 2521 aatctttttt tatttttatt taagaatggc ttaataaagc ggttactttg gatttttgtg 2581 agcttggact agaaaaaaac ttcacaaaat gctatactag gtaggtaaaa aaatattcgg 2641 aggaattttg aaatggcaat cgtttcagca gaaaaattcg taattcgagc tcgcccgggg 2701 atcgatcctc tagagtcgac ctgcaggcat gcaagcttgc aaagtctgaa aacgaaggtg 2761 gcagctgccg ttgaagcggc caagacagtt ggtaaaggcg acggtacaac cggtactagc 2821 gacaaaggcg gcggtcaagg taccccggcg ctacgatatt tggagttgag gttcaaagtc 2881 aaatggtact gatgaccggt aaaatttaat attttgaacc ttgcttaggc agctgacttc 2941 acattgttga gatcagctgc cttttgctta tagttcattg agtagaaacg gttctgttgc 3001 gaagtttgaa aatcaaacgc aagctcgatt ttttattaaa acgtctcaaa atcgtttctg 3061 agacgtttta gcgtttattt cgtttagtta tcggcataat cgttaaaaca ggcgttatcg 3121 tagcgtaaaa gcccttgagc gtagcgtggc tttgcagcga agatgttgtc tgttagatta 3181 tgaaagccga tgactgaatg aaataataag cgcagcgccc ttctatttcg gttggaggag 3241 gctcaaggga gtatgaggga atgaaattcc ctcatgggtt tgattttaaa aattgcttgc 3301 aattttgccg agcggtagcg ctggaaaatt tttgaaaaaa atttggaatt tggaaaaaaa 3361 tggggggaaa ggaagcgaat tttgcttccg tactacgacc ccccattaag tgccgagtgc 3421 caatttttgt gccaaaaacg ctctatccca actggctcaa gggtttaagg ggtttttcaa 3481 tcgccaacga atcgccaacg ttttcgccaa cgttttttat aaatctatat ttaagtagct 3541 ttattgttgt ttttatgatt acaaagtgat acactaactt tataaaatta tttgattgga 3601 gttttttaat // |
2楼2019-07-26 08:10:05
snoopyzxx
木虫 (著名写手)
- MolEPI: 4
- 应助: 49 (小学生)
- 金币: 3232.6
- 散金: 1266
- 红花: 43
- 帖子: 2256
- 在线: 276.6小时
- 虫号: 548497
- 注册: 2008-04-19
- 性别: MM
- 专业: 全球变化生态学
3楼2019-07-26 08:10:41
4楼2019-07-26 12:22:27