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Abstract Fermentation extracts from 1,075 endophytic fungi were screened by the Markwardt method. The endophytic strain CPCC 480097 had the strongest antithrombotic activity and was identified as Fusarium sp. based on morphologic tests and internal transcriptional spacer sequence analysis. The target of the antithrombotic agent from the endophytic strainCPCC480097 was identified by analysis of the fibrinogen clotting time, amidolytic activity, and fibrinolytic assay. The results showed that this antithrombotic agent is a 28-kDa single-chain fibrinolytic enzyme. Theidentification of this fibrinolytic enzyme was performed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). The two internal sequences of this enzyme were obtained, and these showed no homology with those of other known fibrinolytic enzymes. |
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3楼2009-05-27 16:02:08
★ ★ ★ ★ ★ ★ ★ ★ ★ ★
fjtcm8772(金币+5,VIP+0): 5-27 18:43
fjtcm8772(金币+5,VIP+0): 5-29 08:30
fjtcm8772(金币+5,VIP+0): 5-27 18:43
fjtcm8772(金币+5,VIP+0): 5-29 08:30
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Abstract Fermentation extracts from 1,075 endophytic fungi were screened by the Markwardt method. The endophytic strain CPCC 480097 had the strongest antithrombotic activity and was identified as Fusarium sp. based on morphologic tests and internal transcriptional spacer sequence analysis. 摘要 采用Markwardt法对1075种菌类植物发酵物进行提取。基于形态测试和内部基因组合分析，菌株植物CPCC 480097由于具有最好的抗血栓性能，因此被用作镰刀霉。 |
2楼2009-05-27 15:19:33
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