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西山小子

新虫 (小有名气)

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虫号: 4206282
注册: 2015-11-09
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专业: 食品科学基础

[求助] 1、4 vol of 0.1 M NaCl什么意思2、含0.6 M NaCl的磷酸盐缓冲液 (50mM, pH6.0)配置

题目：Then, the myofibrillar pellets were washed three more times with 4 volof 0.1 M NaCl under the same conditions as mentioned above except that in the last wash，the myofibrillar suspension was filtered through a 40 mesh sifter to remove connective tissue and its pH was adjusted to 6.25 (simulating the pH conditions in processed meats) with 0.1 M HCl prior to centrifugation.
然后,用4 vol of 0.1 M NaCl对肌原纤维微颗粒进行三次洗涤，除了最后那次洗涤外，,用40目筛子过滤肌纤维悬浮液去除结缔组织，离心前用0.1 M HCl将其pH值调整为6.25（模拟加工肉类的pH值）。
问题：里面的4 vol of 0.1 M NaCl是什么意思？
题目：Finally, the pellets were suspended in PBS (50 mM[], pH6.0) containing 0.6 M NaCl and the concentration of protein was determined by Biuret with BSA as the standard.
最后，将肌原纤维颗粒悬浮于含0.6 M NaCl的磷酸盐缓冲液 (50mM, pH6.0)中，以BSA为标准，用双缩脲法测定蛋白浓度。
问题：含0.6 M NaCl的磷酸盐缓冲液 (50mM, pH6.0)，配置是不是用0.6mol/l的NaCl作为溶剂配置磷酸盐缓冲液，里面的ph值，是用NaOH调的吗？

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