| 查看: 223 | 回复: 2 | |||
| 当前主题已经存档。 | |||
xiaoyue1199木虫 (著名写手)
第三军团情报局长
|
[交流]
【分享】钱永健小组合成出红外线荧光蛋白
|
||
|
Mammalian Expression of Infrared Fluorescent Proteins Engineered from a Bacterial Phytochrome Xiaokun Shu,1,2 Antoine Royant,3 Michael Z. Lin,2 Todd A. Aguilera,2 Varda Lev-Ram,2 Paul A. Steinbach,1,2 Roger Y. Tsien1,2,4,*  新红外线蛋白质的结构，小图是活体小鼠发出的红外线。 ABSTRACT:Visibly fluorescent proteins (FPs) from jellyfish and corals have revolutionized many areas of molecular and cell biology, but the use of FPs in intact animals, such as mice, has been handicapped by poor penetration of excitation light. We now show that a bacteriophytochrome from Deinococcus radiodurans, incorporating biliverdin as the chromophore, can be engineered into monomeric, infrared-fluorescent proteins (IFPs), with excitation and emission maxima of 684 and 708 nm, respectively; extinction coefficient >90,000 M–1 cm–1; and quantum yield of 0.07. IFPs express well in mammalian cells and mice and spontaneously incorporate biliverdin, which is ubiquitous as the initial intermediate in heme catabolism but has negligible fluorescence by itself. Because their wavelengths penetrate tissue well, IFPs are suitable for whole-body imaging. The IFPs developed here provide a scaffold for further engineering. 原文摘要部分报道： http://www.nature.com/nature/jou ... ll/nature07981.html |
回复此楼» 猜你喜欢
308求调剂
已经有3人回复
-
求b区院校调剂
已经有5人回复
-
0854AI CV方向招收调剂
已经有4人回复
-
296求调剂
已经有7人回复
-
302求调剂
已经有4人回复
-
086000生物与医药292求调剂
已经有5人回复
-
北科281学硕材料求调剂
已经有14人回复
-
材料与化工 322求调剂
已经有5人回复
-
333求调剂
已经有11人回复
-
材料专硕 335 分求调剂
已经有3人回复
xiaoyue1199
木虫 (著名写手)
第三军团情报局长
- 应助: 1 (幼儿园)
- 金币: 3674.4
- 散金: 2164
- 红花: 2
- 帖子: 1348
- 在线: 630小时
- 虫号: 443065
- 注册: 2007-10-27
- 专业: 应用高分子化学与物理
2楼2009-05-14 17:36:49
hongkaichen
金虫 (小有名气)
- 应助: 0 (幼儿园)
- 金币: 801.7
- 帖子: 136
- 在线: 22.2小时
- 虫号: 168376
- 注册: 2006-01-16
- 性别: GG
- 专业: 生物大分子结构与功能
3楼2009-05-14 20:38:29
6