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Model low-complexity microarray hybridization with biotin human elongation factor (HEF)-DNA detected with avidin-Cy5 and laser scanning (right panel) and subsequent detection with biotinpolyethylene glycol (Bio-PEG) UC phosphor (left panel). Concentrations of HEF probe DNA solutions used for spotting are given next to the spots (500–0.25 ng ml1). Concentrations along the left side refer to the first and third columns, whereas those on the right refer to the second and fourth columns. Arrows indicate detection sensitivity. (Reprinted with permission from ref. 49. |
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