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7楼2009-04-29 16:36:52
springimu
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3楼2009-04-27 19:21:31
4楼2009-04-27 20:25:29
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Titre du document / Document title Determination of cell-wall porosity by microscopy: walls of cultured cells and pollen tubes Auteur(s) / Author(s) O'DRISCOLL D. (1) ; READ S. M. ; STEER M. W. (1) ; Affiliation(s) du ou des auteurs / Author(s) Affiliation(s) (1) Univ. coll. Dublin, dep. botany, Dublin 4, IRLANDE Résumé / Abstract In previous studies the porosity of the plant cell wall has been estimated to lie between 3.5 and 6 nm, depending on the cell type investigated and the method used. Morinda citrifolia cells from cultures at various stages of growth were incubated for 60 min with a colloidal gold sol (CGS) of size range 1.9-7.9 nm. Penetration of the cell wall by these gold particles was limited to the log phase of the culture growth cycle, with maximum uptake occurring in early log phase. The size of particles observed within the early log phase cells ranged from 2.5 to 5.7 nm. Since the cell wall was not, as in other methods, disrupted or altered, we conclude that the wall pores are up to 5.7 nm mean diameter at this early stage, but decrease rapidly as the culture ages Revue / Journal Title Acta botanica neerlandica ISSN 0044-5983 CODEN ABNRAN Source / Source Congrès Cell wall meeting No6, Nijmegen , PAYS-BAS (25/08/1992) 1993, vol. 42, no2, pp. 237-244 (16 ref.) Langue / Language Anglais Editeur / Publisher Royal Botanical Society of the Netherlands, Amsterdam, PAYS-BAS (1952-1998) (Revue) Mots-clés anglais / English Keywords Pollen tube ; Cell wall ; Cell permeability ; Ultrastructure ; Cell culture ; Microscopy ; Physical properties ; Porosity ; Nicotiana ; Fluorescent labelling ; Stimulant plant ; Solanaceae ; Dicotyledones ; Angiospermae ; Spermatophyta ; Commelinaceae ; Monocotyledones ; Rubiaceae ; Mots-clés français / French Keywords Tube pollinique ; Paroi cellulaire ; Perméabilité cellulaire ; Ultrastructure ; Culture cellulaire ; Microscopie ; Propriété physique ; Porosité ; Nicotiana ; Marquage fluorescent ; Tradescantia ; Morinda citrifolia ; Plante stimulante ; Solanaceae ; Dicotyledones ; Angiospermae ; Spermatophyta ; Commelinaceae ; Monocotyledones ; Rubiaceae ; Mots-clés espagnols / Spanish Keywords Tubo polínico ; Pared celular ; Permeabilidad celular ; Ultraestructura ; Cultivo celular ; Microscopía ; Propiedad física ; Porosidad ; Nicotiana ; Marcación fluorescente ; Planta estimulante ; Solanaceae ; Dicotyledones ; Angiospermae ; Spermatophyta ; Commelinaceae ; Monocotyledones ; Rubiaceae ; Localisation / Location INIST-CNRS, Cote INIST : 7138, 35400003492833.0140 |
5楼2009-04-29 16:34:11













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