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柳中袁铁杆木虫 (小有名气)
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植物细胞细胞壁允许通过的离子直径范围
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DYNAMICS OF LIMITING CELL WALL POROSITY IN PLANT SUSPENSION CULTURES Changes in the limiting porosity of cell walls, i.e. the size limit for permeation of neutral molecules through the wall, were studied in several higher-plant cell-suspension cultures. For this purpose, samples of biomass fixed at different cultivation times were investigated using a method based on size-exclusion chromatography of polydisperse dextrans before and after equilibration with the extracted cell clusters. In suspension cultures of Chenopodium album L., Dioscorea deltoidea Wall. and Medicago sativa L., the mean size limit (MSL: critical Stokes' radius for exclusion of neutral polymers from half of the intracellular space) was found to vary between 2.4 and 3.8 nm. It decreased significantly during transition from the growth phaseto the stationary phase. In the case of the C. album culture this change was found to be irrespective of whether sucrose in the medium was completely depleted at the end of the growth phase or not. The MSL was kept constant for long periods of the stationary phase if cell viability was maintained by repeated sucrose supplement. In a suspension strain of Triticum aestivum L., the MSL of cell wall permeation was comparatively small (1.75 nm) and remained constant during all cultivation phases. Relations between limiting porosity and cell wall growth, loss of pectic compounds to the medium, crosslinking activities and cell wall stiffening are discussed. [References: 32] |
6楼2009-04-29 16:34:38
springimu
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3楼2009-04-27 19:21:31
4楼2009-04-27 20:25:29
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Titre du document / Document title Determination of cell-wall porosity by microscopy: walls of cultured cells and pollen tubes Auteur(s) / Author(s) O'DRISCOLL D. (1) ; READ S. M. ; STEER M. W. (1) ; Affiliation(s) du ou des auteurs / Author(s) Affiliation(s) (1) Univ. coll. Dublin, dep. botany, Dublin 4, IRLANDE Résumé / Abstract In previous studies the porosity of the plant cell wall has been estimated to lie between 3.5 and 6 nm, depending on the cell type investigated and the method used. Morinda citrifolia cells from cultures at various stages of growth were incubated for 60 min with a colloidal gold sol (CGS) of size range 1.9-7.9 nm. Penetration of the cell wall by these gold particles was limited to the log phase of the culture growth cycle, with maximum uptake occurring in early log phase. The size of particles observed within the early log phase cells ranged from 2.5 to 5.7 nm. Since the cell wall was not, as in other methods, disrupted or altered, we conclude that the wall pores are up to 5.7 nm mean diameter at this early stage, but decrease rapidly as the culture ages Revue / Journal Title Acta botanica neerlandica ISSN 0044-5983 CODEN ABNRAN Source / Source Congrès Cell wall meeting No6, Nijmegen , PAYS-BAS (25/08/1992) 1993, vol. 42, no2, pp. 237-244 (16 ref.) Langue / Language Anglais Editeur / Publisher Royal Botanical Society of the Netherlands, Amsterdam, PAYS-BAS (1952-1998) (Revue) Mots-clés anglais / English Keywords Pollen tube ; Cell wall ; Cell permeability ; Ultrastructure ; Cell culture ; Microscopy ; Physical properties ; Porosity ; Nicotiana ; Fluorescent labelling ; Stimulant plant ; Solanaceae ; Dicotyledones ; Angiospermae ; Spermatophyta ; Commelinaceae ; Monocotyledones ; Rubiaceae ; Mots-clés français / French Keywords Tube pollinique ; Paroi cellulaire ; Perméabilité cellulaire ; Ultrastructure ; Culture cellulaire ; Microscopie ; Propriété physique ; Porosité ; Nicotiana ; Marquage fluorescent ; Tradescantia ; Morinda citrifolia ; Plante stimulante ; Solanaceae ; Dicotyledones ; Angiospermae ; Spermatophyta ; Commelinaceae ; Monocotyledones ; Rubiaceae ; Mots-clés espagnols / Spanish Keywords Tubo polínico ; Pared celular ; Permeabilidad celular ; Ultraestructura ; Cultivo celular ; Microscopía ; Propiedad física ; Porosidad ; Nicotiana ; Marcación fluorescente ; Planta estimulante ; Solanaceae ; Dicotyledones ; Angiospermae ; Spermatophyta ; Commelinaceae ; Monocotyledones ; Rubiaceae ; Localisation / Location INIST-CNRS, Cote INIST : 7138, 35400003492833.0140 |
5楼2009-04-29 16:34:11
