±±¾©Ê¯ÓÍ»¯¹¤Ñ§Ôº2026ÄêÑо¿ÉúÕÐÉú½ÓÊÕµ÷¼Á¹«¸æ
²é¿´: 5950  |  »Ø¸´: 2

medicilon

Í­³æ (СÓÐÃûÆø)


[½»Á÷] dzÎöRNA¸ÉÈż¼ÊõÔÚ»ùÒòÇóýÉϵÄÓ¦ÓÃ

»ùÒòÇóýÊÇÒ»ÏîÖØÒªµÄ·Ö×ÓÉúÎïѧ¼¼Êõ£¬Ð¼¼ÊõÈçCRISPR/Cas9»ùÒò±à¼­¼¼ÊõµÄÔËÓÿÉÏÔÖø½µµÍ»ùÒòÇóý¼Û¸ñºÍÌá¸ß³É¹¦ÂÊ¡£»ùÒòÇóý¿ÉÀûÓÃ΢ÉúÎïÌåÄÚµÄÍ¬Ô´ÖØ×éϵͳ£¬ÔÚÒ»¶¨Ñ¡ÔñѹÁ¦ÏÂʹÌåÍâ¸ÄÔìµÄij¹¦ÄÜ»ùÒòÓëÊÜÌåϸ°ûȾɫÌåÉϵŦÄÜ»ùÒòÖ®¼ä·¢ÉúÍ¬Ô´ÖØ×飬´Ó¶ø¸Ä±äϸ°ûµÄÒÅ´«ÌØÐÔ¡£RNA¸ÉÈÅ£¨RNA interference,RNAi£©£¬ÓÖ³Æ×ªÂ¼ºó»ùÒò³ÁĬ£¬ÊÇÖ¸½«ÌØÒìÐÔͬԴ˫Á´RNAµ¼È뵽ϸ°ûÄÚ£¬Ê¹Ä¿µÄ»ùÒòµÄ²»±í´ï»ò±í´ïˮƽϽµ¡£Ó봫ͳµÄ»ùÒòÇóý¼¼ÊõÏà±È£¬RNAi¼¼Êõ¾ßÓÐͶÈëÉÙ¡¢ÖÜÆÚ¶Ì¡¢²Ù×÷¼òµ¥µÈÓÅÊÆ¡£
Ò»°ãÀ´Ëµ»ùÒòÇóý¼Û¸ñ½Ï¸ß£¬¸ù¾ÝÊг¡±ê¼Û£¬ÊµÑéС°×Êóÿֻ1.5Ôª£¬´ôÃȵÄС²ÖÊó9.9Ôª°üÓÊ£¬Äö³ÝÄ¿µÄÇ×ÆÝ¡¢Ô²¹ö¹öµÄ¿É°®ÁúèҲ²»³¬¹ýǧԪ¡£µ«ÊÇ£¬»ùÒòÇóýСÊó´òÁËÆßÕÛºó»¹Òª½«½ü¼¸ÍòÔª¡£
ÀûÓûùÒòÇóý¼¼Êõ¿ÉÒÔ×è¶Ïϸ°ûµÄ´úлÅÔ·£¬»òͨ¹ýÒýÈëÍ»±äλµã¸Ä±äÄ¿µÄ²úÎïµÄ²úÁ¿»òÖÊÁ¿£¬´Ó¶ø´ïµ½Î¢ÉúÎïÓýÖÖµÄÄ¿µÄ¡£ÔÚÖйúcro¹«Ë¾ÅÅÃûÖп¿Ç°µÄÃÀµÏÎ÷ÔÚ·Ö×ÓÉúÎïѧ¡¢Ï¸°ûÉúÎïѧ¡¢ÌåÍâÉúÎïѧ¡¢½á¹¹ÉúÎïѧµÈÉúÎïѧÁìÓò¾ßÓзḻ¹ã·ºµÄ¾­Ñ飬ͨ¹ýøˮƽ¡¢Ï¸°ûˮƽ²â¶¨µÈ¼¼Êõƽ̨°ïÖú¿Í»§½øÐл¯ºÏÎïɸѡ¼°»¯ºÏÎïÌØÐÔ¡¢×÷ÓûúÖÆ¼°ÉúÎï±ê¼ÇÎï·ÖÎöµÈÑо¿£¬±£Ö¤Á˿ͻ§ÏîÄ¿µÄÓÐÁ¦ÊµÊ©£¬°ïÖú¿Í»§ÍƽøÒ©ÎïÑз¢µÄ½ø³Ì¡£
1¡¢»ùÒòÇóýÑо¿µÄ×÷ÓÃ
ÉúÃüÌåÓÉÎÞÊý¿éµ°°×ÖÊÆ´Í¼×é³É£¬ÎªÁËÈ·ÈÏijÖÖµ°°×ÖÊÔÚÉúÃüÏÖÏóÖз¢»ÓµÄ×÷Óã¬×îÖ±½ÓµÄ°ì·¨¾ÍÊÇÏñ²ðµçÊÓÒ»Ñù£¬°ÑÆäÖÐÒ»¸öÁã¼þ²ðÏÂÀ´£¬¿´¿´µçÊÓÓÐʲô·´Ó¦¡£Ëæ×Å¿ÆÑ§½ø²½£¬Ñо¿ÈËÔ±¿ª·¢³öÁËÈËÎªÖÆÔì±äÒìÌåµÄ·½·¨£¬ÈËÎªÆÆ»µ»ùÒò²¢·ÖÎöÆäºó¹û£¬Õâ¾ÍÊÇËùνµÄ¡°»ùÒòÇóýʵÑ顱¡£ÒÔ·Ö×ÓÉúÎïѧµÄÉúÃü¹Û¿´À´£¬ÉúÃüÌåÊÇÓÉÎÞÊý΢СµÄÁ㲿¼þ×é×°³ÉµÄ¾«ÃÜÄ£ÐÍ£¬ÊÇÒ»²¿¡°·Ö×Ó»úÆ÷¡±£¬¡°»ùÒòÇóýʵÑ顱ÊÇÈ÷Ö×Ó»úÆ÷ij¸öÁã¼þÍ£Ö¹¹¤×÷£¬×Ðϸ¹Û²ìÉúÃüÌåÔÚÕâÖÖ״̬Ï»á²úÉúÔõÑùµÄÒì³££¬½ø¶ø·ÖÎö¸ÃÁã¼þÓкÎÖÖ×÷Óá£
ÔÚºó»ùÒò×éʱ´ú£¬´óÁ¿Î´Öª»ùÒòµÄ¹¦ÄܵÄÑо¿ÏÔµÃÓÈÆäÆÈÇУ¬ÓÉÓÚ»ùÒò×é×÷ΪÉúÃüÌåµÄ»ù±¾À¶Í¼£¬ËüµÄÍêÕû¹¦ÄÜÖ»ÓÐÔÚÍêÕûµÄÉúÎï¸öÌå¼°ÆäÕû¸öÉúÃü¹ý³ÌÖвÅÄܵõ½×îÍêÕûÈ·ÇеÄÌåÏÖ£¬ÓÈÆäÔڸߵÈÉúÎïÖУ¬Ï¸°û¼äµÄÏ໥×÷ÓÃÒÔ¼°Éñ¾­¡¢ÄÚ·ÖÃÚ¡¢ÃâÒßµ÷¿ØÍøÂçµÈ¶Ô»ùÒòµÄÕý³£ÉúÎïѧ¹¦Äܶ¼¾ßÓÐÖØÒªµÄÓ°Ï죬Òò´Ë£¬ÔÚÉúÎïÌåÄÚÑо¿»ùÒò×éµÄ¹¦Äܽ«ÊÇÒ»¸ö±ØÈ»µÄÑ¡Ôñ¡£ÓÃÓÚСÊóÄ£Ð͹¹½¨µÄת»ùÒò¼¼ÊõºÍ»ùÒòÇóý¼¼ÊõÒѳÉΪ»ùÒòÑо¿µÄÖØÒªÊֶΣ¬ÓÈÆäÊÇÌõ¼þÐÔ»ùÒòÇóý¼¼Êõ¿ÉÒÔÔÚʱÎʺͿռäÉϵ÷½Ú»ùÒòµÄ±í´ï£¬´Ó¶ø·Ç³£ÓÐЧµÄ¶Ô»ùÒò¹¦ÄܽøÐÐÑо¿¡£
µ«ÊÇÕâÏî¼¼Êõ´æÔÚ×ŲÙ×÷¸´ÔÓ¡¢·ÑʱºÍ°º¹óµÈȱµã¡£RNA¸ÉÈż¼ÊõµÄ³öÏÖΪ½â¾öÕâ¸öÎÊÌâÖ¸Ã÷ÁË·½Ïò¡£ÏÖÔÚÆÕ±éÈÏΪת¼ºó»ùÒò³ÁĬÊÇÉúÎï±£³Ö»ùÒòµÄÍêÕûÐÔ£¬µÖÓùÍâÀ´ºËËáÈëÇÖ£¬ÒÖÖÆ×ª×ùÒò×ÓÓÕµ¼DNAÍ»±äµÄÊֶΣ¬¹ã·º´æÔÚÓÚÕæ¾ú¡¢Ö²Îï¡¢Ï߳桢¼¹×µ¶¯ÎïºÍ´ó³¦¸Ë¾úÖС£
2¡¢Ê²Ã´ÊÇRNA¸ÉÈż¼Êõ  
RNA¸ÉÈÅÊÇÒ»ÖÖÆÕ±éµÄת¼ºó»ùÒò³ÁĬµÄ»úÖÆ£¬ÓÉsiRNA(smallintereferenceRNA)ÒýÆð£¬siRNAÊÇÍâÔ´»ùÒò²úÉúµÄdsRNAÔÚDicerøµÄ×÷ÓÃÏÂËù²úÉúµÄ³¤¶ÈΪ21-22ntµÄһϵÁÐÆ¬¶ÎµÄ×ܳƣ¬¾ßÓкÜÇ¿µÄ¹Ø±Õ»ùÒòµÄ¹¦ÄÜ,Äܹ»½éµ¼RNA¸ÉÉæÏÖÏó£¬ÓÕµ¼³öÒ»ÖÖÓÉsiR2NA·Ö×Ó¡¢ºËËáøÒÔ¼°ÂÝÐýøµÈ½áºÏÔÚÒ»ÆðµÄRNAÓÕµ¼³ÁĬ¸´ºÏÎï(RNA-inducedsilencingcomplex,RISC)¡£RISCÄܹ»½â¿ªsiRNA²¢¾«È·µÄÖ¸µ¼ÌØÒìµÄmRNA½µ½â£¬Ê¹Ï¸°ûµÖ¿¹ÍâÀ´»ùÒòÇÖÈë¼°²¡¶¾¸ÐȾ¡£Òò´Ë£¬Í¨¹ý½«dsRNA·Ö×Óµ¼Èëϸ°ûÄÚ£¬ÌØÒìÐԵؽµ½âϸ°ûÄÚÓëÆäͬԴµÄmRNA£¬·â±ÕÄÚÔ´ÐÔ»ùÒòµÄ±í´ïÀ´Ê§»î¸Ã»ùÒòͬÑù¿ÉÒÔʵÏÖ»ùÒòµÄÇóý¡£
1998Äê,RNAi¼¼ÊõÊ×ÏÈÔÚÏß³æÖн¨Á¢,ĿǰÒÑÔÚÏ߳桢¹ûÓ¬¡¢Õæ¾ú¼°ÄâÄϽæµÈÉúÎïÖзֱð½¨Á¢,²¢ÓÃÓÚÑо¿Ìض¨»ùÒò»òÒÑÖª»ùÒòÔÚÌØ¶¨·¢ÓýʱÆÚµÄ¹¦ÄÜ¡£
3¡¢RNA¸ÉÈż¼ÊõµÄ×÷ÓÃÌØµã  
RNAiµÄ×÷ÓÃÌØµãÓÐ:
(1)¾ßÓи߶ȵÄÌØÒìÐÔ£¬Õë¶ÔͬԴ»ùÒò¹²ÓÐÐòÁеÄRNAiÔòÖ»Äܵ¼ÖÂͬԴ»ùÒòʧ»î£¬²»Ó°ÏìÆäËûÄÚÔ´ÐÔmRNAµÄ±í´ï£»
(2)ЧÂʸߣ¬ÉÙÁ¿µÄdsRNA·Ö×ӾͿÉÒÔÍêÈ«ÒÖÖÆÏàÓ¦»ùÒòµÄ±í´ï£¬ÄÜÔÚµÍÓÚ·´ÒåºËËἸ¸öÊýÁ¿¼¶µÄŨ¶ÈÏÂÑо¿Ä¿µÄ»ùÒò£»
(3)´©Í¸ÐÔÇ¿£¬RNAiÒÖÖÆ»ùÒò±í´ïµÄЧӦ¿ÉÒÔ´©¹ýϸ°û½çÏÞ,ÔÚ²»Í¬Ï¸°û¼ä³¤¾àÀë´«µÝºÍά³Ö¡£
RNAi¼¼Êõ²Ù×÷Ïà¶Ô¼ò±ã£¬½á¹û¿ì½Ý£¬ÓÈÆä¶ÔÓÚÄÇЩ²»ÈÝÒ×»ñµÃÍ»±äÌåµÄ»ùÒò»òÉúÎïÌåÀ´Ëµ£¬RNAi¼¼ÊõÎÞÒÉÌṩÁËÒ»ÖÖ¿ìËÙÓÐЧµÄÑо¿»ùÒò¹¦Äܵķ½·¨¡£ÓÉÓÚdsRNAÒýÈëÉúÎïÌåÄڵļÁÁ¿ºÍʱ¼ä¿ÉÒÔÈËΪ¿ØÖÆ£¬RNAi¼¼Êõ¿ÉÒÔÓÃÓÚÑо¿Ä³¸öÌØ¶¨»ùÒòÔÚÌØ¶¨·¢Óý½×¶ÎµÄ¹¦ÄÜ¡£°éËæ×Ź¦ÄÜ»ùÒò×éѧÑо¿µÄÉîÈ룬RNAi¼¼ÊõÔÚÑо¿ÐźŴ«µ¼Í¾¾¶¡¢ÅßÌ¥·¢ÓýÏà¹ØÒò×ÓÒÔ¼°²ÎÓë»ù±¾ÉúÎï·´Ó¦¹ý³ÌµÄлùÒò¹¦ÄÜ·½ÃæÓо޴ó·¢Õ¹Ç±Á¦£¬Ëü½«´øÀ´ÉúÎïѧÑо¿µÄ¸ïÃü¡£
4¡¢RNA¸ÉÈż¼ÊõµÄȱµã
ËäÈ»RNAi¼¼ÊõÓÐÈç´ËÃ÷ÏÔµÄרһÐÔÓŵ㣬µ«RNAi²»ÄÜ×÷ÓÃÓÚËùÓлùÒòºÍijЩϸ°ûÀàÐÍ(ÈçÉñ¾­Ôª)£¬ºÜ¶àÉè¼ÆµÄdsRNA²»ÄܲúÉúÒÖÖÆ°Ð»ùÒòת¼ºó³ÁĬµÄЧ¹û¡£ÓÉÓÚRNAiʵ¼ÊÉÏÖ»ÊÇÔÚת¼ºóˮƽÉϽµµÍ»ùÒòµÄ±í´ï£¬²¢²»Ïó»ùÒòÇóýÄÇÑùÍêÈ«°Ñ»ùÒò´Ó»ùÒò×éÖÐÌÞ³ýµô£¬ÓÐʱҲ»áÒò±³¾°µÄ²»¸É¾»µ¼Ö²úÉúµÄ±íÐÍÄÑÒÔ·ÖÎö¡£Òò´Ë,RNAi²»ÄÜÍêȫȡ´ú´«Í³µÄ»ùÒòÇóý¼¼Êõ¡£
Ëæ×Ź¦ÄÜ»ùÒò×éѧµÄÐËÆðºÍ·¢Õ¹£¬»ùÒòÇóý¼¼Êõ±»ÈËÃǹ㷺µÄÓÃÓÚ̽ÌÖ»ùÒò¹¦ÄܵÄÑо¿£¬Í¨¹ý»ùÒòÇóý¼¼ÊõÖð½¥Ã÷È·ÁË´óÁ¿Ä£Ê½ÉúÎïºÍÖØÒª¹¦ÄÜ΢ÉúÎïµÄÈ«»ùÒò×飬²¢ÄÜ׼ȷ¶¨Î»¹¦ÄÜ»ùÒò£¬´Ó»ùÒòˮƽÉϸü¼ÓÇå³þµÄÈÏʶ΢ÉúÎïµÄ´úл¹æÂÉ¡£Í¬Ê±£¬ÇóýÔØÌå¼°Ïà¹Ø¼¼ÊõµÄÍêÉÆ¿É½øÒ»²½Íƶ¯Î¢ÉúÎï´úл¹¤³ÌµÄ¹ã·ºÓ¦Óã¬Í¨¹ý»ùÒòÇóýÊֶθıä΢ÉúÎïϸ°ûµÄ´úлÁ÷Ïò£¬×è¶ÏÓк¦´úл²úÎï»ýÀÛ£¬¿ÉÍû½µµÍ³É±¾£¬´ó·ù¶ÈÌá¸ßÉú²úˮƽ¼°²úÆ·´¿¶È¡£
»Ø¸´´ËÂ¥

» ²ÂÄãϲ»¶

» ±¾Ö÷ÌâÏà¹ØÉ̼ÒÍÆ¼ö: (ÎÒÒ²ÒªÔÚÕâÀïÍÆ¹ã)

ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
2Â¥2019-01-24 08:25:45
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

С³æ²Ý¾ú

гæ (³õÈëÎÄ̳)

ÄÚÈÝÒÑɾ³ý
3Â¥2019-02-22 15:13:41
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
Ïà¹Ø°æ¿éÌø×ª ÎÒÒª¶©ÔÄÂ¥Ö÷ medicilon µÄÖ÷Ìâ¸üÐÂ
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼ÑÐ] 304Çóµ÷¼Á +4 ËØÄê¼ÀÓï 2026-03-31 4/200 2026-03-31 12:40 by -ÃÔÁË·°¡Â·
[¿¼ÑÐ] 288Çóµ÷¼Á Ò»Ö¾Ô¸¹þ¹¤´ó ²ÄÁÏÓ뻯¹¤ +7 ÂåÉñ¸ç¸ç 2026-03-31 7/350 2026-03-31 12:29 by ̽123
[¿¼ÑÐ] 070300»¯Ñ§Çóµ÷¼Á +8 С»ÆÑ¼±¦ 2026-03-30 8/400 2026-03-31 12:24 by ÌÆãå¶ù
[¿¼ÑÐ] 294·Ö080500²ÄÁÏ¿ÆÑ§Ó빤³ÌÇóµ÷¼Á +9 ÁøÏª±ß 2026-03-26 9/450 2026-03-31 12:16 by oooqiao
[¿¼ÑÐ] Ò»Ö¾Ô¸Î÷µç085401ÊýÒ»Ó¢Ò»299Çóµ÷¼Á Áù¼¶521 +4 °®³Ô´óѼÀæ 2026-03-31 4/200 2026-03-31 11:51 by ²«»÷518
[¿¼ÑÐ] ÍÁľ304Çóµ÷¼Á +4 ÍÃͻͻͻ£¬ 2026-03-31 5/250 2026-03-31 11:29 by ±±·çÖ®Éñ.
[¿¼ÑÐ] 282Çóµ÷¼Á ²»Ìôרҵ ÇóÊÕÁô +3 Yam. 2026-03-30 4/200 2026-03-31 10:04 by cal0306
[¿¼ÑÐ] 08¹¤¿Æ£¬295£¬½ÓÊÜ¿çרҵµ÷¼Á +6 lmnlzy 2026-03-30 6/300 2026-03-31 10:04 by cal0306
[¿¼ÑÐ] 0856µ÷¼Á +6 ÇúÌýóÞ 2026-03-30 6/300 2026-03-31 09:44 by Jaylen.
[¿¼ÑÐ] 0710ÉúÎïѧÇóµ÷¼Á£¡ +3 ÐðÊöÎÄ 2026-03-31 3/150 2026-03-31 09:35 by longlotian
[¿¼ÑÐ] Ò»Ö¾Ô¸Öк£Ñó320»¯Ñ§¹¤³ÌÓë¼¼Êõѧ˶Çóµ÷¼Á +8 ÅûÐÇºÓ 2026-03-30 8/400 2026-03-31 08:53 by lbsjt
[¿¼ÑÐ] 303Çóµ÷¼Á +7 DLkz1314. 2026-03-30 7/350 2026-03-30 21:07 by peike
[¿¼ÑÐ] Ò»Ö¾Ô¸ÏÃÃÅ´óѧ²ÄÁϹ¤³Ìר˶354ÕÒµ÷¼Á£¡£¡£¡ +5 ±´ß±µ±µ 2026-03-30 5/250 2026-03-30 18:16 by Î޼ʵIJÝÔ­
[¿¼ÑÐ] ÉúÎï¼¼ÊõÓ빤³Ì +7 1294608413 2026-03-25 8/400 2026-03-30 11:36 by ÌÆãå¶ù
[¿¼ÑÐ] 349Çóµ÷¼Á +6 Àîľ×Ó°¡¹þ¹þ 2026-03-25 6/300 2026-03-29 12:47 by Î޼ʵIJÝÔ­
[˶²©¼ÒÔ°] ÕÐÊÕÉúÎïѧ/ϸ°ûÉúÎïѧµ÷¼Á +4 IceGuo 2026-03-26 5/250 2026-03-29 01:25 by griffith2014
[¿¼ÑÐ] 315·ÖÇóµ÷¼Á +7 26¿¼ÑÐÉϰ¶°æ26 2026-03-26 7/350 2026-03-28 04:05 by fmesaito
[¿¼ÑÐ] 0856µ÷¼Á +5 ÇóÇóÈÃÎÒÓÐÊé¶Á° 2026-03-26 6/300 2026-03-27 15:12 by caszguilin
[ÂÛÎÄͶ¸å] Journal of Mechanical Science and Technology +3 Russ_ss 2026-03-25 5/250 2026-03-27 10:49 by ½С¹û»­´ó±ý
[¿¼ÑÐ] ²ÄÁÏר˶ 335 ·ÖÇóµ÷¼Á +4 ¾Ü¾øÀ䱩Á¦ 2026-03-25 4/200 2026-03-25 18:45 by haxia
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û