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LIYUANDONG

木虫 (著名写手)

应助: 11 (小学生)
金币: 4464.9
散金: 59
红花: 8
帖子: 1313
在线: 458.4小时
虫号: 599187
注册: 2008-09-11
专业: 色谱分析

[交流] 求助：关于过柱子！！！

Silica Gel Fractionation. Final extracts were separated on 10 g of
silica gel (60F254 from Merck) placed in a 10 mm diameter buret
plugged with deactivated glass wool. Elutions were first carried out
with 20 mL of pentane and then successively with 10 mL of various
n-pentane/dichloromethane mixtures (80/20, 60/40, 40/60, 20/80) and
finally 20 mL of pure dichloromethane. Sixteen fractions (labeled in
order of elution from 1A to 16A) each containing 5 mL were
successively recovered and then reduced to 200 íL using a Kuderna-
Danish column. Each fraction was analyzed by GC-MS

上面的一段话，是我在文献上面看见的，是柱分离化合物，但是现在我关键是不懂，这里用的调料是硅胶还是中性氧化铝？
就是第一句话不是很懂？？？？？？？
60F254是中性氧化铝的板子
用中性氧化铝的板子跑板子，那么装柱子的时候一定要用中性氧化铝填料吗？
希望大侠指点一下

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