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yidoudewunv

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Temperature and humidity alter prolactin receptor expression in the skin
of toad (Bufo bankorensis and Bufo melanostictus)
温度和湿度改变催乳素受体在蟾蜍皮肤中的表达（盘古蟾蜍和黑眶蟾蜍）

Bufo bankorensis and Bufo melanostictus, the only two species of Bufonidae genus in Taiwan, live in habitats that differ in altitude and
humidity. This study tested the hypothesis that prolactin receptor (PRLR) expression responds to environmental change. Western blot analysis
showed that the PRLR protein was widely distributed in brain, lung, liver, kidney, dorsal skin and ventral skin of toads. The level PRLR protein
was elevated in the dorsal skin of the two toad species treated with dry or wet conditions for 14 days. The increase in PRLR of dorsal skin in B.
bankorensis was higher than that in B. melanostictus. This experimental result suggests that B. bankorensis secretes more mucus to reduce water
evaporation from its thinner cuticle than B. melanostictus. The expression of PRLR protein was increased in the lung of B. bankorensis and
decreased in the lung of B. melanostictus. Moreover, PRLR protein levels were increased in the kidneys in the two species toad, likely due to
reduction in water lost through lung and urine. The two toad species were subjected to varying temperatures (25 °C, 15 °C and 10 °C) for 14 days.
The lowest PRLR protein expression was observed at 10 °C. Comparison of the decreasing trend in PRLR protein levels demonstrated that the
variation in B. bankorensis was significantly higher than that in B. melanostictus. Comparisons of variation in PRLR protein expression in the two
species under different environments suggest that B. bankorensis is more adaptable to different environments than B. melanostictus.
© 2006 Published by Elsevier Inc.

摘要：盘古蟾蜍和黑眶蟾蜍是台湾仅剩的两个蟾蜍品种，它们栖息在不同的海拔高度和湿度的地方。这项研究设立的假设是催乳素受体（PRLR）的表达响应环境的变化。Western blot分析表明， PRLR蛋白是广泛分布于蟾蜍的脑，肺，肝，肾，背部和腹部的皮肤。经过在干燥或潮湿的条件下14天的处理，两个蟾蜍品种背部皮肤的PRLR蛋白量均升高。盘古蟾蜍背部皮肤中的PRLR增加量高于黑眶蟾蜍。这一实验结果表明：盘古蟾蜍比黑眶蟾蜍分泌更多的粘液以减少水分从它们的薄角质层蒸发。盘古蟾蜍的肺中的PRLR蛋白表达增加，而黑眶蟾蜍肺中的PRLR蛋白表达减少。另外，两个品种的蟾蜍肾脏的PRLR蛋白量都增加，很可能是为了肺中水分的减少和尿液的减少。将这两个品种蟾蜍放在不同的温度（25 °C, 15 °C和10 °C）下14天，观察到10 °C下PRLR蛋白的表达最低。比较PRLR蛋白量下降的趋势，证明盘古蟾蜍变异明显高于黑眶蟾蜍。比较这两个品种的蟾蜍在不同的环境下PRLR蛋白的表达的变化，表明盘古蟾蜍比黑眶蟾蜍更能适应不同的环境。

以上是自己翻的摘要翻译，总感觉有些不对，希望哪位高手帮忙翻译全文
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1楼 2009-04-16 16:55:37

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yidoudewunv

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Originally posted by yidoudewunv at 2009-4-16 16:55:
Temperature and humidity alter prolactin receptor expression in the skin
of toad (Bufo bankorensis and Bufo melanostictus)
温度和湿度改变催乳素受体在蟾蜍皮肤中的表达（盘古蟾蜍和黑眶蟾蜍）

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4楼2009-04-16 17:38:50

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mayong11

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Temperature and humidity alter prolactin receptor expression in the skin
of toad (Bufo bankorensis and Bufo melanostictus)
温度和湿度能够改变催乳素受体在蟾蜍（盘古蟾蜍和黑眶蟾蜍）
皮肤中的表达

摘要：盘古蟾蜍和黑眶蟾蜍是台湾仅有的两个蟾蜍物种，它们分别栖息在海拔高度和湿度不同的地方. 这项研究调查了催乳素受体（PRLR）的表达对于环境的变化响应。蛋白印迹分析表明，催乳素受体蛋白广泛分布于蟾蜍的脑，肺，肝，肾，皮肤和背腹部的皮肤。这一实验结果表明盘古蟾蜍的薄角质层比黑眶蟾蜍的的薄角质层分泌更多的粘液以减少水分蒸发。因此催乳素蛋白在盘古蟾蜍的肺中表达增加，而在黑眶蟾蜍肺中的表达减少。另外，研究结果还表明催乳素蛋白在两个品种的蟾蜍肾脏中的表达都增加，很可能是由于肺和肾中水分损失的减少引起的。这两个蟾蜍物种在14天里受到不同温度（ 25 ℃ ，15 ℃和10 ℃ ）的影响，其中观察到10 ° C时，催乳素蛋白的表达量最低。比较催乳素蛋白在两种蟾蜍的皮肤中表达水平下降的变化趋势，证明盘古蟾蜍明显高于黑眶蟾蜍。比较不同的环境下，催乳素蛋白在两种蟾蜍的皮肤中表达的变化，表明盘古蟾蜍比黑眶蟾蜍更能适应不同的环境。

[ Last edited by mayong11 on 2009-4-16 at 18:08 ]