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| Calibration-Starting with the lowest concentration standard,analyze each calibration standard 3 times according to the conditions listed above.(please note,it important to analyze the standards in order of increasing concentration.This is done to prevent any bias that might occur due to carryover from the high concentration standard).After calibrating,inject H2O repeatedly until a clean chromatogram is obtained(i.e.area for EG and DEG is <20% of the areas seen from standard H).set up the data acquisition system to perform a linear calibration based upon analysis of these standards |
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