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求高人帮我翻译下这段话
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Silver nanoparticles (1, see Scheme 1). were preparedusing a modified Lee–Meisel method with an average size of 50 nm and a final silver concentration of 1 mM. The suspending mediumwas a 1 mM sodium citrate solution. To prepare the silica-coated silver nanoparticles (2), the silver nanoparticles (1) were concentrated by centrifugation and added to a solution of 2.5 mM TEOS in ethanol for a final silver concentration of 6.25 mM. After mixing, a small volume of ammonia was added to a final concentration of 0.7% by weight to catalyze the reaction and the reaction vessel was placed on an orbital shaker for 1 h to ensure continuous mixing. The suspension was then diluted 4 times with 1 mM sodium citrate and the suspending medium removed after centrifugation. The silica-coated silver nanoparticles (2) were resuspended in ethanol and stored in darkness at roomtemperature. To prepare the positively charged silica-coated silver nanoparticles (3), the silica-coated nanoparticles (2) were added to a LoBind Eppendorf tube containing 1.5 mM TMA in ethanol. The reaction was allowed to proceed at room temperature for 10 min before the addition of a dilute solution of ammonia (0.035% w: v) as a catalyst. After a further 10 min, the nanoparticles were centrifuged at 4℃ and then resuspended in 1 mM HCl. To prepare silica particles, 100 or 200 μl of 10% TEOS in ethanol was added to a mixture of 4.4 ml water and 15 ml ethanol. Then 0.5 ml 28% ammonia was added to catalyze the reaction. The solution was agitated for 60 min at room temperature. The suspension was diluted 4 times with 1 mM sodium citrate. The silica particles were collected by centrifugation and resuspended in ethanol. Observation under TEM showed that the silica particles have a size range from 50 to 100 nm. To prepare positively charged silica particles, the same procedure as that for silica-coated silver nanoparticles was used. |
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xiaoshuo6006(金币+5,VIP+0):还可以 4-29 17:46
qingfeng877680(金币+10,VIP+0):MM翻译辛苦了！ 7-21 13:58
xiaoshuo6006(金币+5,VIP+0):还可以 4-29 17:46
qingfeng877680(金币+10,VIP+0):MM翻译辛苦了！ 7-21 13:58
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银纳米颗粒（1，见图1）通过改进的Lee–Meisel方法合成，平均粒径为50nm，最终的银浓度为1 mM。悬浊溶液为1 mM的柠檬酸钠溶液。为了制备硅-涂覆的银纳米颗粒（2），将纳米颗粒（1）通过离心浓缩，加入到2.5mM的TEOS的乙醇溶液中，使得最终银的浓度为6.25 mM。混合后，加入少量的氨水（最终的溶液中氨水的重量百分比为0.7%）催化反应，反应容器置于搅拌器中1个小时，以保证持续混合。然后用1 mM的柠檬酸钠溶液对上述悬浊液进行稀释至4倍，离心后将悬浊液除去。得到的硅-涂覆的银纳米颗粒（2）分散于乙醇中形成悬浊液，于室温和避光条件下储存。 为了制备正电荷硅-涂覆银纳米颗粒（3），将硅-涂覆的银纳米颗粒（2）加入到含有1.5 mM的TMA乙醇溶液的LoBind Eppendorf 试管中。室温下，反应进行10分钟后，加入稀释的氨水溶液（0.035%）催化反应。再过十分钟后，纳米颗粒在4℃条件下离心分离，然后重新分散于 1 mM HCl溶液中。 为了合成硅颗粒，100或者200uLTEOS的乙醇溶液（TEOS含量10%）加入到4.4ml水和15ml乙醇的混合溶液中。然后，加入0.5 ml28%的氨水催化反应进行。室温下溶液搅拌60分钟。然后，用1 mM的柠檬酸钠对悬浊液稀释4倍。硅颗粒经过离心过滤得到并重新分散至乙醇中。TEM观察显示，硅颗粒大小为50-100nm。采用与制备硅涂覆银纳米颗粒的相同的方法来合成正电荷硅颗粒（？） 好辛苦，币币不多呀。最后一句话不太对吧？楼主自己斟酌 |
2楼2009-04-08 15:00:52