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卖糖葫芦: 金币+500, ★★★★★最佳答案, 就是我要的 2018-09-06 11:12:36
卖糖葫芦: 金币+500, ★★★★★最佳答案, 就是我要的 2018-09-06 11:12:36
| Haematococcus accumulates up to 5% (by dry weight) of fatty acid-esterified astaxanthin and is considered as an important source of natural astaxanthin production. Precise quantification of astaxanthin is needed to evaluate the quality of microalgal sources for astaxanthin extraction and to monitor the Haematococcus cultivation process. In the present study, an efficient approach of astaxanthin measurement has been developed. Dimethyl sulfoxide was used as an extraction solvent to improve the membrane permeability of Haematococcus. Cholesterol esterase was used to hydrolyze astaxanthin esters via incubation at 36 °C for 30 min. The detection of astaxanthin after hydrolysis was achieved by HPLC within 10 min using a C18 column. The astaxanthin content measurements after enzymatic pretreatment were 1.5-1.7 times higher than that measured after saponified pretreatment. DMSO solvent extraction combined with lipase-catalyzed hydrolysis followed by HPLC detection is proposed for the accurate quantification of astaxanthin in Haematococcus. |
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