| 查看: 683 | 回复: 2 | ||
卖糖葫芦木虫 (著名写手)
|
[求助]
翻译文献
|
|
求翻译，私信聊 发自小木虫Android客户端 |
回复此楼» 猜你喜欢
E0414, 我的本子有没有希望？
已经有7人回复
-
有谁可曾问过你过的还好吗？
已经有17人回复
-
一篇论文同时出现在两个期刊，一模一样，这算不算学术不端，请各位老师斧正。
已经有12人回复
-
希望面上有个好结果
已经有7人回复
-
今年也是没消息就是没中么
已经有16人回复
-
三区计算机方向期刊推荐
已经有5人回复
-
sci论文二审求助
已经有5人回复
-
函评
已经有7人回复
-
买卖文章的刷屏了！
已经有3人回复
【答案】应助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ...
卖糖葫芦: 金币+500, ★★★★★最佳答案, 就是我要的 2018-09-06 11:12:36
卖糖葫芦: 金币+500, ★★★★★最佳答案, 就是我要的 2018-09-06 11:12:36
| Haematococcus accumulates up to 5% (by dry weight) of fatty acid-esterified astaxanthin and is considered as an important source of natural astaxanthin production. Precise quantification of astaxanthin is needed to evaluate the quality of microalgal sources for astaxanthin extraction and to monitor the Haematococcus cultivation process. In the present study, an efficient approach of astaxanthin measurement has been developed. Dimethyl sulfoxide was used as an extraction solvent to improve the membrane permeability of Haematococcus. Cholesterol esterase was used to hydrolyze astaxanthin esters via incubation at 36 °C for 30 min. The detection of astaxanthin after hydrolysis was achieved by HPLC within 10 min using a C18 column. The astaxanthin content measurements after enzymatic pretreatment were 1.5-1.7 times higher than that measured after saponified pretreatment. DMSO solvent extraction combined with lipase-catalyzed hydrolysis followed by HPLC detection is proposed for the accurate quantification of astaxanthin in Haematococcus. |
» 本帖已获得的红花（最新10朵）
卖糖葫芦2楼2018-09-06 10:06:38
3楼2018-09-06 10:10:08