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toney1984

铁虫 (初入文坛)

[交流] 35S启动子

花椰菜花叶病毒的35S启动子以及35SRNA中的35S表示的是什么意思,查了一些资料并没有太详细的解释,35S在这里仅是指沉降系数吗,有哪位高人能指点下,谢谢!
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小猫三两只

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2楼2009-04-04 16:47:46
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sutao1979

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toney1984(金币+1,VIP+0): 5-20 16:02
At the beginning of the 1980s, Chua and collaborators at the Rockefeller University isolated the promoter responsible for the transcription of the whole genome of a Cauliflower mosaic virus (CaMV) infecting turnips. The promoter was named CaMV 35S promoter ("35S promoter" because the coefficient of sedimentation of the viral transcript whose expression is naturally driven by this promoter is 35S. It is one of the most widely used, general-purpose constitutive promoters.

The 35S promoter is a very strong constitutive promoter, causing high levels of gene expression in dicot plants. However, it is less effective in monocots, especially in cereals. The differences in behavior are probably due to differences in quality and/or quantity of regulatory factors.
coefficient of sedimentation 沉降系数

[ Last edited by sutao1979 on 2009-4-4 at 17:50 ]
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3楼2009-04-04 17:49:29
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